Highly Sensitive and Diversified Electrochemiluminescence DNA Methylation Biosensing Platform Based on Self-Assembly of Nanotags DOI
Xu Cao, Yuan Chen, Qian Yu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

This work proposes a highly sensitive, simple, and reliable electrochemiluminescence (ECL) DNA methylation biosensing platform by employing DNA-functionalized magnetic beads (DNA-MBs) for target capture nanotag self-assembly aggregation signal amplification. The methylated was first captured on DNA-MBs through base pairing recognition, then its sites were recognized antibody-5mC (Ab-5mC). Afterward, pair of antibodies functionalized [Ru(byp)3]2+-doped silica nanoparticles (Ab2-Ru@SiO2 Ab3-Ru@SiO2) layer-by-layer assembled Ab-5mC amplified transduction. sensing could be transferred to screen-printed carbon electrodes (SPCEs) ECL curve detection via photomultiplier tube or gold-coated indium tin oxide (Au/ITO) arrays high-throughput imaging detection. As the assembly layers increased from 1 3, sensitivities SPCE-based Au/ITO-based enhanced 7-fold 3-fold, achieving limits down 0.8 pM 0.9 fM, respectively. nanotags showed good stability, with storage times 300 days Ru@SiO2 60 Ab-Ru@SiO2, method is universal applied detect different DNAs using their corresponding DNA-MBs. proposed possessed advantages high sensitivity, diversity, practicality, showing potential in clinical diagnosis.

Language: Английский

DNAzyme@MOF breaking pH limitation for the detection of dopamine in the interstitial fluid DOI

Jiuhong Zhao,

Jun‐Wu Zhao, Xiaoyu Zhang

et al.

Biosensors and Bioelectronics, Journal Year: 2025, Volume and Issue: unknown, P. 117367 - 117367

Published: March 1, 2025

Language: Английский

Citations

1
Multilayered MXene electrodes in deep eutectic solvent ionic liquid electrolyte for supercapacitor applications DOI
Derya Kapusuz, Abdulcabbar Yavuz

Journal of Alloys and Compounds, Journal Year: 2025, Volume and Issue: unknown, P. 179442 - 179442

Published: Feb. 1, 2025

Citations

0
Closed-loop cascade nanozyme strategy for mutually reinforced catalytic and mild-temperature photothermal therapeutic effects DOI Creative Commons
Fan Yang,

Chunyu Yan,

Nannan Li

et al.

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 4, 2025

Nanocatalysis coupled with photothermal therapy is a potent anti-cancer approach, yet its clinical utility limited by low concentration of tumor substrate, redox interference, and risks overheating normal tissues. Herein, we propose an innovative closed-loop nanozyme approach that leverages the synergistic effects catalytic mild (mPTT) to address aforementioned challenges. The strategy features folic acid-functionalized iron single-atom catalyst (FeNC-FA), designed exhibit exceptional multienzymatic capabilities optimal response. In system, engineered FeNC-FA capable inducing reactive oxygen species (ROS) storm depleting glutathione (GSH) in specific microenvironment (TME) initiate ferroptosis. Concurrently, accumulation ROS effectively cleaves heat shock proteins (HSPs), thereby enhancing mPTT. An intriguing aspect increased temperature within TME further facilitates conversion H2O2 O2, alleviating hypoxia providing positive feedback circuit boost therapy. Additionally, advanced photoacoustic (PA) imaging allow for self-monitoring their at sites, guiding mPTT process. Taken together, it provides PA image-guided, mutually reinforced both vitro vivo. This targeted holds great promise personalized medicine applications.

Language: Английский

Citations

0
Recent progress and challenges in ECL biosensors for the identification of small extracellular vesicle and clinical diagnosis DOI
Shijie Li,

Peilin Wang,

Qiang Ma

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 118253 - 118253

Published: April 1, 2025

Language: Английский

Citations

0
Porphyrinic metal-organic framework PCN-224 supported Prussian blue enables selective detection of casein and phytic acid DOI
Zhiqiang Zhou, Xiao Zhang, Wenting Chen

et al.

Talanta, Journal Year: 2025, Volume and Issue: unknown, P. 128121 - 128121

Published: April 1, 2025

Language: Английский

Citations

0
MOF-engineered Cu2O nanozymes with boosted peroxidase-like activity for colorimetric-fluorescent dual-mode detection of deoxynivalenol DOI
Xiao‐Dong Zhu,

Yangchun He,

Xinhua Xie

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(5)

Published: April 24, 2025

Language: Английский

Citations

0
Highly Sensitive and Diversified Electrochemiluminescence DNA Methylation Biosensing Platform Based on Self-Assembly of Nanotags DOI
Xu Cao, Yuan Chen, Qian Yu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

This work proposes a highly sensitive, simple, and reliable electrochemiluminescence (ECL) DNA methylation biosensing platform by employing DNA-functionalized magnetic beads (DNA-MBs) for target capture nanotag self-assembly aggregation signal amplification. The methylated was first captured on DNA-MBs through base pairing recognition, then its sites were recognized antibody-5mC (Ab-5mC). Afterward, pair of antibodies functionalized [Ru(byp)3]2+-doped silica nanoparticles (Ab2-Ru@SiO2 Ab3-Ru@SiO2) layer-by-layer assembled Ab-5mC amplified transduction. sensing could be transferred to screen-printed carbon electrodes (SPCEs) ECL curve detection via photomultiplier tube or gold-coated indium tin oxide (Au/ITO) arrays high-throughput imaging detection. As the assembly layers increased from 1 3, sensitivities SPCE-based Au/ITO-based enhanced 7-fold 3-fold, achieving limits down 0.8 pM 0.9 fM, respectively. nanotags showed good stability, with storage times 300 days Ru@SiO2 60 Ab-Ru@SiO2, method is universal applied detect different DNAs using their corresponding DNA-MBs. proposed possessed advantages high sensitivity, diversity, practicality, showing potential in clinical diagnosis.

Language: Английский

Citations

0