
Journal of Dairy Science, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
The nutrient consumption patterns of Streptococcus thermophilus F7 under acid stress were investigated in this study. strain was incubated at 42°C for 16 h a chemically defined medium with different pH values (6.5, 5.8, and 5.5), variety nutrients including amino acids, vitamins, purine/pyrimidine bases, ions observed. results showed that Leu the most consumed all levels, 2.5, 3.4, 4.5 μmol/cfu × 10-5 6.5, 5.5, respectively. Consumption biotin highest among ranging from 48.3 nmol/cfu 6.5 to 126.7 5.5. At adenine purine (78.3 10-5), whereas uracil became base more stress, 122.5 122.2 5.8 Regarding ion consumption, Na+ predominant (35.4 10-5) (41.0 K+ when dropping 5.5 (61.9 10-5). Certain highly demanded individually added same incubation conditions, revealed optical density 600 nm S. supplemented 1.5 times or 2 isoleucine increased by 1.13 folds compared initial medium. Similarly, supplementation 3 proline resulted 1.15-fold 1.12-fold increase nm, This study significant differences various culturing F7, which had requirement followed ions, pyrimidine bases. Furthermore, individual addition acids bases improvement biomass production strain. findings present provide basis on requirements contribute design media high-cell-density cultivation.
Язык: Английский