Nutrient consumption patterns of Streptococcus thermophilus F7 under acid stress and their application in enhancing biomass production

Journal of Dairy Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

The nutrient consumption patterns of Streptococcus thermophilus F7 under acid stress were investigated in this study. strain was incubated at 42°C for 16 h a chemically defined medium with different pH values (6.5, 5.8, and 5.5), variety nutrients including amino acids, vitamins, purine/pyrimidine bases, ions observed. results showed that Leu the most consumed all levels, 2.5, 3.4, 4.5 μmol/cfu × 10-5 6.5, 5.5, respectively. Consumption biotin highest among ranging from 48.3 nmol/cfu 6.5 to 126.7 5.5. At adenine purine (78.3 10-5), whereas uracil became base more stress, 122.5 122.2 5.8 Regarding ion consumption, Na+ predominant (35.4 10-5) (41.0 K+ when dropping 5.5 (61.9 10-5). Certain highly demanded individually added same incubation conditions, revealed optical density 600 nm S. supplemented 1.5 times or 2 isoleucine increased by 1.13 folds compared initial medium. Similarly, supplementation 3 proline resulted 1.15-fold 1.12-fold increase nm, This study significant differences various culturing F7, which had requirement followed ions, pyrimidine bases. Furthermore, individual addition acids bases improvement biomass production strain. findings present provide basis on requirements contribute design media high-cell-density cultivation.

Language: Английский

Aminic Organoselenium Compounds as Glutathione Peroxidase Mimics and Inhibitors of Ferroptosis

ChemBioChem, Journal Year: 2024, Volume and Issue: 25(4)

Published: Jan. 31, 2024

The synthesis of diarylamine-based organoselenium compounds via the nucleophilic substitution reactions has been described. Symmetrical monoselenides and diselenides were conveniently synthesized by reduction their corresponding selenocyanates using sodium borohydride. Selenocyanates obtained from 2-chloro acetamides displacement with potassium selenocyanate. Selenides treating in situ generated butyl selenolate as nucleophile. Further, newly evaluated for glutathione peroxidase (GPx)-like activity thiophenol assay. This study revealed that methoxy-substituted showed significant effect on GPx-like activity. catalytic parameters most efficient catalysts also determined. anti-ferroptotic all GPx-mimics a 4-OH-tamoxifen (TAM) inducible GPx4 knockout cell line liproxstatin standard.

Language: Английский