Chemoselective Proteomics, Zinc Fingers, and a Zinc(II) Model for H₂S Mediated Persulfidation

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(27)

Опубликована: Май 29, 2024

The gasotransmitter hydrogen sulfide (H

Язык: Английский

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The Function of Zinc in Animal, Plant, and Human Nutrition

Journal for Research in Applied Sciences and Biotechnology, Год журнала: 2023, Номер 2(2), С. 35 - 43

Опубликована: Апрель 8, 2023

Zinc is a crucial micronutrient found in food that essential for the development of all living organisms, it plays vital role gene expression and various physical processes, its deficiency can lead to disease onset reduce crop yield, restrict plant development, lower quality produced goods, zinc also used fertilizers handling metals protect them from oxidation. Additionally, important enhancing human immune system, result hair loss, memory skin disorders, muscular weakness.

Язык: Английский

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Strategies to increase the robustness of microbial cell factories

Advanced Biotechnology, Год журнала: 2024, Номер 2(1)

Опубликована: Март 1, 2024

Engineering microbial cell factories have achieved much progress in producing fuels, natural products and bulk chemicals. However, industrial fermentation, cells often face various predictable stochastic disturbances resulting from intermediate metabolites or end product toxicity, metabolic burden harsh environment. These perturbances can potentially decrease productivity titer. Therefore, strain robustness is essential to ensure reliable sustainable production efficiency. In this review, the current strategies improve host were summarized, including knowledge-based engineering approaches, such as transcription factors, membrane/transporters stress proteins, traditional adaptive laboratory evolution based on selection. Computation-assisted (e.g. GEMs, deep learning machine learning) design of robust hosts was also introduced. Furthermore, challenges future perspectives are proposed promote development green, efficient biomanufacturers.

Язык: Английский

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Peptides and metal ions: A successful marriage for developing artificial metalloproteins

Journal of Peptide Science, Год журнала: 2024, Номер unknown

Опубликована: Май 8, 2024

The mutual relationship between peptides and metal ions enables metalloproteins to have crucial roles in biological systems, including structural, sensing, electron transport, catalytic functions. effort reproduce or/and enhance these roles, or even create unprecedented functions, is the focus of protein design, first step toward comprehension complex machinery nature. Nowadays, design allows building sophisticated scaffolds, with novel functions exceptional stability. Recent progress metalloprotein has led peptides/proteins capable orchestrating desired different cofactors. structural diversity proper selection first- second-shell ligands, as well long-range electrostatic hydrophobic interactions, which represent precious tools for tuning properties. scope this review discuss construction sites de novo designed miniaturized scaffolds. Selected examples mono-, di-, multi-nuclear binding sites, from last 20 years will be described an highlight key artificial models electron-transfer metalloproteins. authors' goal make readers feel like guests at marriage while offering sources inspiration future architects innovative,

Язык: Английский

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An artificial transcription factor that activates potent interferon-γ expression in human Jurkat T Cells

Frontiers in Molecular Medicine, Год журнала: 2025, Номер 4

Опубликована: Янв. 8, 2025

Interferon (IFN)-γ is a central regulator of cell-mediated immunity in human health and disease, but reduced expression the target receptors impairs signaling activity leads to immunotherapy resistance. Although intracellular IFN-γ restores downstream functions, we lack tools activate IFNG gene instead cell surface receptors. This paper introduces design characterization an artificial transcription factor (ATF) protein that recognizes with six zinc finger domains, which are dovetailed VP64 domain promotes translation. Biological studies Jurkat T cells reveal ATF amplifies translation, also stimulates for multiple class I II HLA alleles interferon-stimulated genes (ISGs). Biophysical showed recombinant nanomolar affinity (KD = 5.27 ± 0.3 nM), adopts secondary structure associated ββα-fold resistant thermal denaturation. These demonstrate transcriptional targeting cytokine genes, rather than receptors, activates shows significant potential directing immune function.

Язык: Английский

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Bacterial DNA methylases as novel molecular and synthetic biology tools: recent developments

Applied Microbiology and Biotechnology, Год журнала: 2025, Номер 109(1)

Опубликована: Март 6, 2025

Bacterial DNA methylases are a diverse group of enzymes which have been pivotal in the development technologies with applications including genetic engineering, bacteriology, biotechnology and agriculture. This review describes bacterial methylase types, main for targeted methylation or demethylation recent roles these molecular synthetic biology. can be exocyclic endocyclic exist as orphan part restriction-modifications (R-M) systems. As group, they display rich diversity sequence-specificity. Additional targeting involve using fusion proteins combining DNA-binding protein (DNBP) such zinc-finger (ZF), transcription activator-like effector (TALE) CRISPR/dCas9. contributed significantly to creation novel assembly techniques, improvement transformation crop plant engineering. Future studies define characteristics more potential identify new tools value biology widespread applications. KEY POINTS: • used direct specific sequences target has applied improve increase efficiency Site-selective alter gene expression phenotype.

Язык: Английский

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Genome Editing to Accelerate Biotic Stress Resistance in Crop Plants

CABI eBooks, Год журнала: 2025, Номер unknown, С. 174 - 192

Опубликована: Март 21, 2025

Язык: Английский

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Molecular Functions and Potential Utilization of Zinc Finger Proteins in Protozoan Parasites

Опубликована: Апрель 10, 2025

Zinc finger proteins (ZFP) are metalloproteins whose zinc atom interacts with side chains of specific histidines and cysteines the motif, generating functional three dimensional structures. A good number these from different species similar while others divergent in structure function. Existing literature on structural features, functions their potential to be targeted for drug development or used as molecular tools parasitic protozoa was explored. Evidence reviewed articles revealed that, just like other eukaryotes, function various parasite cellular processes such as: transcription, RNA editing, mRNA processing & export and, turn-over among others. Some them had same features homologues eukaryotes including hosts. However, there were which studies aiming at replacing reported positive results some parasites Giardia lamblia, Trichomonas vaginalis Leishmania donovani though this is promising, off- target challenge that can cause effects, must solved before wide application achieved. Successful engineering use genome editing also Plasmodium falciparum. Since nucleases a powerful research medical tool, repurposing ZFPs useful therapy discovery. For succeed, more knowledge how regulated needed.

Язык: Английский

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Engineering transcriptional regulation for cell-based therapies

SLAS TECHNOLOGY, Год журнала: 2024, Номер 29(2), С. 100121 - 100121

Опубликована: Фев. 8, 2024

A major aim in the field of synthetic biology is developing tools capable responding to user-defined inputs by activating therapeutically relevant cellular functions. Gene transcription and regulation response external stimuli are some most powerful versatile these functions being explored. Motivated success chimeric antigen receptor (CAR) T-cell therapies, transmembrane receptor-based platforms have been embraced for their ability sense extracellular ligands subsequently activate intracellular signal transduction. The integration receptors with transcriptional activation has not yet achieved its full potential. Transient expression plasmid DNA often used explore gene vitro. However, applications targeting endogenous or stably integrated genes more clinically relevant. may allow engineered cells traffic into tissues interest secrete functional proteins space differentiate cells. Transmembrane that regulate potential revolutionize cell therapies a myriad applications, including cancer treatment regenerative medicine. In this review, we will examine current engineering approaches control mammalian an emphasis on systems can be selectively activated signals. We also speculate therapeutic technologies promising expand capabilities tighten therapies.

Язык: Английский

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Mining proteomes for zinc finger persulfidation

RSC Chemical Biology, Год журнала: 2024, Номер 5(6), С. 572 - 585

Опубликована: Янв. 1, 2024

A meta-analysis of 22 persulfide-specific proteomics datasets reveals widespread persulfidation zinc finger proteins across various species, highlighting the role as an important post-translational modification.

Язык: Английский

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