Platelet-rich fibrin as an autologous biomaterial for bone regeneration: mechanisms, applications, optimization

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Апрель 16, 2024

Platelet-rich fibrin, a classical autologous-derived bioactive material, consists of fibrin scaffold and its internal loading growth factors, platelets, leukocytes, with the gradual degradation slow release physiological doses factors. PRF promotes vascular regeneration, proliferation migration osteoblast-related cells such as mesenchymal cells, osteoblasts, osteoclasts while having certain immunomodulatory anti-bacterial effects. has excellent osteogenic potential been widely used in field bone tissue engineering dentistry. However, there are still some limitations PRF, improvement biological properties is one most important issues to be solved. Therefore, it often combined scaffolds enhance mechanical delay degradation. In this paper, we present systematic review development platelet-rich derivatives, structure mechanisms, applications, optimization broaden their clinical applications provide guidance for translation.

Язык: Английский

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Clinical translation of personalized bioengineered implant scaffolds

Nature Reviews Bioengineering, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

Язык: Английский

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Efficacy of biologics for the treatment of periodontal infrabony defects: An American Academy of Periodontology best evidence systematic review and network meta‐analysis

Journal of Periodontology, Год журнала: 2022, Номер 93(12), С. 1803 - 1826

Опубликована: Окт. 24, 2022

Abstract Background A large variety of biomaterials, biologics and membranes have been utilized in the past 40 years for regenerative treatment periodontal infrabony defects. Biologic agents progressively gained popularity among clinicians are routinely used regeneration. In alignment with goals American Academy Periodontology (AAP) Best Evidence Consensus (BEC) on use biologic mediators contemporary clinical practice, aim this sytematic review was to evaluate effect agents, specifically autogenous blood‐dervied products (ABPs), enamel matrix derivative (EMD) recombinant human platelet‐derived growth factor‐BB (rhPDGF‐BB), outcomes Methods detailed systematic search conducted identify eligible randomized control trials (RCTs) reporting therapy using frequentist mixed‐modeling approach network meta‐analysis (NMA), characterized by assessment three individual components an defect (the bone graft material [BG], agent, application a barrier membrane) performed compare relative efficacy different components, therapeutic modalities Results total 153 RCTs were included, 150 studies contributing NMA. The quantitative analysis showed that addition significantly improves radiographic outcomes, as compared BG flap procedures alone. Barrier enhanced but did not provide further benefits combination biologics. type (autogenous, allogeneic, xenogeneic or alloplastic) agent (EMD, platelet‐rich fibrin [PRF], plasma [PRP] rhPDGF‐BB) played significant role final Allogeneic BGs exhibited statistically superior gain than synthetic (p < 0.05 all comparisons), while rhPDGF‐BB PRF demonstrated higher stability gingival margin 0.01) fill/gain 0.05), together greater, although significant, attachment level pocket depth reduction, EMD PRP. Overall, largest size most parameters, including gain, less recession linear gain. Considering relatively high number presenting unclear risk bias, strength recommendation supporting PRP judged weak, EMD, deemed favor. Conclusions Biologics enhance therapy. Combination therapies involving + membrane be monotherapies. choice seems impact

Язык: Английский

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Periodontal Wound Healing and Regeneration: Insights for Engineering New Therapeutic Approaches

Frontiers in Dental Medicine, Год журнала: 2022, Номер 3

Опубликована: Март 2, 2022

Periodontitis is a widespread inflammatory disease that leads to loss of the tooth supporting periodontal tissues. The few therapies available regenerate tissues have high costs and inherent limitations, inspiring development new approaches. Studies shown an capacity for regeneration, driven by multipotent cells residing in ligament (PDL). purpose this review describe current understanding mechanisms driving wound healing regeneration can inform treatment biologic basis underlying established such as guided tissue (GTR) growth factor delivery are reviewed, along with examples biomaterials been engineered improve effectiveness these Emerging those targeting Wnt signaling, cell or recruitment, scaffolds described context healing, using key vivo studies illustrate impact approaches on formation cementum, alveolar bone, PDL. Finally, design principles engineering suggested which build knowledge regeneration.

Язык: Английский

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Do autologous platelet concentrates (APCs) have a role in intra‐oral bone regeneration? A critical review of clinical guidelines on decision‐making process

Periodontology 2000, Год журнала: 2023, Номер 93(1), С. 254 - 269

Опубликована: Окт. 1, 2023

Abstract In the past decades, personalized regenerative medicine has gained increased attention. Autologous platelet concentrates (APCs) such as PRP, PRGF, and L‐PRF, all serving a source of large variety cells growth factors that participate in hard soft tissue healing regeneration, could play significant role periodontal procedures. This narrative review evaluated relative impact APCs alveolar ridge preservation, sinus floor augmentation, regeneration bony craters around teeth, both single substitute or combination with xenograft. L‐PRF beneficial effect on preservation (<alveolar bone resorption, >bone quality). The data for PRGF are less convincing, PRP is controversial. can successfully be used during transcrestal (≥3.5 mm gain) well 1‐stage lateral window elevation (>5 gain). For especially very scarce. treatment open flap debridement, showed adjunctive benefits (e.g., >PPD reduction, >CAL gain, >crater depth reduction). were non‐conclusive. Adding to xenograft OFD resulted additional improvements (>PPD fill), no found. demonstrated enhance most convincing. also advantage greater simplicity production, its 100% autologous character.

Язык: Английский

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Nanomaterials for Periodontal Tissue Regeneration: Progress, Challenges and Future Perspectives

Journal of Functional Biomaterials, Год журнала: 2023, Номер 14(6), С. 290 - 290

Опубликована: Май 24, 2023

Bioactive nanomaterials are increasingly being applied in oral health research. Specifically, they have shown great potential for periodontal tissue regeneration and substantially improved translational clinical applications. However, their limitations side effects still need to be explored elucidated. This article aims review the recent advancements discuss future research directions this field, especially focusing on using improve health. The biomimetic physiochemical properties of such as metals polymer composites described detail, including alveolar bone, ligament, cementum gingiva. Finally, biomedical safety issues application regenerative materials updated, with a discussion about complications perspectives. Although applications bioactive cavity at an initial stage, pose numerous challenges, suggests that promising alternative regeneration.

Язык: Английский

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Regeneration of periodontal intrabony defects using platelet-rich fibrin (PRF): a systematic review and network meta-analysis

Odontology, Год журнала: 2024, Номер 112(4), С. 1047 - 1068

Опубликована: Май 21, 2024

Abstract One of the most promising approaches to correct periodontal bone defects and achieve regeneration is platelet-rich fibrin (PRF). This systematic review meta-analysis aimed evaluate using PRF compared other regenerative treatments. The data search retrieval process followed PRISMA guidelines. An electronic MEDLINE, Cochrane, PubMed databases was performed, selecting exclusively randomized clinical trials where following were measured: probing depth reduction (PD), attachment level gain (CAL), radiographic fill (RBF). Out 284 selected articles, 32 chosen based on inclusion criteria. use (PRF) + open flap debridement (OFD), metformin, plasma (PRP), OFD/bone graft (BG) significantly reduced PD improved CAL RBF. However, combination BG, STATINS CAL. intervention combined with different treatments such as OFD, PRP, has a significant impact improving

Язык: Английский

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The next generation of regenerative dentistry: From tooth development biology to periodontal tissue, dental pulp, and whole tooth reconstruction in the clinical setting

Regenerative Therapy, Год журнала: 2025, Номер 28, С. 333 - 344

Опубликована: Янв. 13, 2025

Язык: Английский

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Low‐Speed Platelet‐Rich Fibrin Membrane in Conjunction With Demineralized Freeze‐Dried Bone Allograft (DFDBA) Compared to Collagen Membrane With DFDBA in Noncontained Intraosseous Defects of Stage III Periodontitis: A Randomized Controlled Clinical Trial

International Journal of Dentistry, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Aim: Noncontained (1‐ or combined 1‐ to 2‐wall) periodontal intraosseous defects represent challenging clinical situations with unpredictable surgical therapeutic outcomes. This randomized trial assessed demineralized freeze‐dried bone allograft (DFDBA) low speed‐platelet‐rich fibrin (PRF) membrane compared DFDBA collagen (CM) in the therapy of noncontained stage III periodontitis patients. Methodology: Twenty‐two patients measuring ≥3 mm and attachment loss ≥5 were randomly allocated into two groups: test group (low‐speed PRF + DFDBA) control (CM DFDBA), 11 participants per group. Clinical radiographic assessments conducted at baseline, 3, 6, 9, 12 months for level (CAL; primary outcome), gingival recession depth (GRD), probing (PD), full mouth bleeding score (FMBS) plaque (FMPS), fill linear defect (RLDD; all secondary outcomes). Results: The mean (±SD) CAL‐gain was 2.45 (±1.51), 2.91 (±1.70), (±1.87), 2.82 (±1.83) mm, while (±1.25), 3.27 (±1.27), 3.00 (±1.41), 2.64 (±1.50) months, respectively, no significant intergroup differences ( p > 0.05). Despite absence differences, both groups demonstrated intragroup improvement CAL‐ PD‐gain, RLDD‐reduction as well RLDD < Conclusion: membranes, conjunction DFDBA, show parameters, comparable CMs DFDBA. Trial Registration: ClinicalTrials.gov identifier: NCT03922503

Язык: Английский

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Injectable platelet-rich fibrin with demineralized freeze-dried bone allograft compared to demineralized freeze-dried bone allograft in intrabony defects of patients with stage-III periodontitis: a randomized controlled clinical trial

Clinical Oral Investigations, Год журнала: 2023, Номер 27(7), С. 3457 - 3467

Опубликована: Март 31, 2023

Abstract Aim The current randomized controlled clinical trial assessed the effect of injectable platelet-rich fibrin (I-PRF) combined with demineralized freeze-dried bone allograft (DFDBA) compared to DFDBA alone in management intrabony defects stage-III periodontitis patients. Methodology Following sample size calculation, twenty patients ≥ 5 mm attachment level (CAL)-loss and 3 were into test (I-PRF + DFDBA; n = 10) control (DFDBA; groups. CAL (primary outcome), periodontal probing depth (PPD), gingival recession (GRD), full-mouth plaque scores (FMPS), bleeding (FMBS), radiographic linear defect (RLDD), fill (secondary outcomes) examined at baseline, 3, 6, 9 months post-surgically. Results I-PRF independently demonstrated significant intragroup CAL-gain, PPD-, RLDD-reduction ( p < 0.05), no intergroup differences observed > 0.05). CAL-gain (mean ± SD) 2.40 0.70 2.50 0.85 PPD-reduction 3.50 1.18 2.80 0.42 for respectively. Both groups showed RLDD improvement, a 3.58 0.66 3.89 1.57 Stepwise regression analysis revealed that baseline predictors Conclusion Within present study’s limitations, or without resulted improvement parameters surgical treatment Addition does not appear significantly enhance DFDBA’s reparative/regenerative outcomes. Clinical relevance routinely adding cannot be recommended improve outcomes defects.

Язык: Английский

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