Platelet-rich fibrin as an autologous biomaterial for bone regeneration: mechanisms, applications, optimization

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: April 16, 2024

Platelet-rich fibrin, a classical autologous-derived bioactive material, consists of fibrin scaffold and its internal loading growth factors, platelets, leukocytes, with the gradual degradation slow release physiological doses factors. PRF promotes vascular regeneration, proliferation migration osteoblast-related cells such as mesenchymal cells, osteoblasts, osteoclasts while having certain immunomodulatory anti-bacterial effects. has excellent osteogenic potential been widely used in field bone tissue engineering dentistry. However, there are still some limitations PRF, improvement biological properties is one most important issues to be solved. Therefore, it often combined scaffolds enhance mechanical delay degradation. In this paper, we present systematic review development platelet-rich derivatives, structure mechanisms, applications, optimization broaden their clinical applications provide guidance for translation.

Language: Английский

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The next generation of regenerative dentistry: From tooth development biology to periodontal tissue, dental pulp, and whole tooth reconstruction in the clinical setting

Regenerative Therapy, Journal Year: 2025, Volume and Issue: 28, P. 333 - 344

Published: Jan. 13, 2025

Language: Английский

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Clinical translation of personalized bioengineered implant scaffolds

Nature Reviews Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Language: Английский

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Efficacy of biologics for the treatment of periodontal infrabony defects: An American Academy of Periodontology best evidence systematic review and network meta‐analysis

Journal of Periodontology, Journal Year: 2022, Volume and Issue: 93(12), P. 1803 - 1826

Published: Oct. 24, 2022

Abstract Background A large variety of biomaterials, biologics and membranes have been utilized in the past 40 years for regenerative treatment periodontal infrabony defects. Biologic agents progressively gained popularity among clinicians are routinely used regeneration. In alignment with goals American Academy Periodontology (AAP) Best Evidence Consensus (BEC) on use biologic mediators contemporary clinical practice, aim this sytematic review was to evaluate effect agents, specifically autogenous blood‐dervied products (ABPs), enamel matrix derivative (EMD) recombinant human platelet‐derived growth factor‐BB (rhPDGF‐BB), outcomes Methods detailed systematic search conducted identify eligible randomized control trials (RCTs) reporting therapy using frequentist mixed‐modeling approach network meta‐analysis (NMA), characterized by assessment three individual components an defect (the bone graft material [BG], agent, application a barrier membrane) performed compare relative efficacy different components, therapeutic modalities Results total 153 RCTs were included, 150 studies contributing NMA. The quantitative analysis showed that addition significantly improves radiographic outcomes, as compared BG flap procedures alone. Barrier enhanced but did not provide further benefits combination biologics. type (autogenous, allogeneic, xenogeneic or alloplastic) agent (EMD, platelet‐rich fibrin [PRF], plasma [PRP] rhPDGF‐BB) played significant role final Allogeneic BGs exhibited statistically superior gain than synthetic (p < 0.05 all comparisons), while rhPDGF‐BB PRF demonstrated higher stability gingival margin 0.01) fill/gain 0.05), together greater, although significant, attachment level pocket depth reduction, EMD PRP. Overall, largest size most parameters, including gain, less recession linear gain. Considering relatively high number presenting unclear risk bias, strength recommendation supporting PRP judged weak, EMD, deemed favor. Conclusions Biologics enhance therapy. Combination therapies involving + membrane be monotherapies. choice seems impact

Language: Английский

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Periodontal Wound Healing and Regeneration: Insights for Engineering New Therapeutic Approaches

Frontiers in Dental Medicine, Journal Year: 2022, Volume and Issue: 3

Published: March 2, 2022

Periodontitis is a widespread inflammatory disease that leads to loss of the tooth supporting periodontal tissues. The few therapies available regenerate tissues have high costs and inherent limitations, inspiring development new approaches. Studies shown an capacity for regeneration, driven by multipotent cells residing in ligament (PDL). purpose this review describe current understanding mechanisms driving wound healing regeneration can inform treatment biologic basis underlying established such as guided tissue (GTR) growth factor delivery are reviewed, along with examples biomaterials been engineered improve effectiveness these Emerging those targeting Wnt signaling, cell or recruitment, scaffolds described context healing, using key vivo studies illustrate impact approaches on formation cementum, alveolar bone, PDL. Finally, design principles engineering suggested which build knowledge regeneration.

Language: Английский

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Injectable platelet-rich fibrin with demineralized freeze-dried bone allograft compared to demineralized freeze-dried bone allograft in intrabony defects of patients with stage-III periodontitis: a randomized controlled clinical trial

Clinical Oral Investigations, Journal Year: 2023, Volume and Issue: 27(7), P. 3457 - 3467

Published: March 31, 2023

Abstract Aim The current randomized controlled clinical trial assessed the effect of injectable platelet-rich fibrin (I-PRF) combined with demineralized freeze-dried bone allograft (DFDBA) compared to DFDBA alone in management intrabony defects stage-III periodontitis patients. Methodology Following sample size calculation, twenty patients ≥ 5 mm attachment level (CAL)-loss and 3 were into test (I-PRF + DFDBA; n = 10) control (DFDBA; groups. CAL (primary outcome), periodontal probing depth (PPD), gingival recession (GRD), full-mouth plaque scores (FMPS), bleeding (FMBS), radiographic linear defect (RLDD), fill (secondary outcomes) examined at baseline, 3, 6, 9 months post-surgically. Results I-PRF independently demonstrated significant intragroup CAL-gain, PPD-, RLDD-reduction ( p < 0.05), no intergroup differences observed > 0.05). CAL-gain (mean ± SD) 2.40 0.70 2.50 0.85 PPD-reduction 3.50 1.18 2.80 0.42 for respectively. Both groups showed RLDD improvement, a 3.58 0.66 3.89 1.57 Stepwise regression analysis revealed that baseline predictors Conclusion Within present study’s limitations, or without resulted improvement parameters surgical treatment Addition does not appear significantly enhance DFDBA’s reparative/regenerative outcomes. Clinical relevance routinely adding cannot be recommended improve outcomes defects.

Language: Английский

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Nanomaterials for Periodontal Tissue Regeneration: Progress, Challenges and Future Perspectives

Journal of Functional Biomaterials, Journal Year: 2023, Volume and Issue: 14(6), P. 290 - 290

Published: May 24, 2023

Bioactive nanomaterials are increasingly being applied in oral health research. Specifically, they have shown great potential for periodontal tissue regeneration and substantially improved translational clinical applications. However, their limitations side effects still need to be explored elucidated. This article aims review the recent advancements discuss future research directions this field, especially focusing on using improve health. The biomimetic physiochemical properties of such as metals polymer composites described detail, including alveolar bone, ligament, cementum gingiva. Finally, biomedical safety issues application regenerative materials updated, with a discussion about complications perspectives. Although applications bioactive cavity at an initial stage, pose numerous challenges, suggests that promising alternative regeneration.

Language: Английский

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Do autologous platelet concentrates (APCs) have a role in intra‐oral bone regeneration? A critical review of clinical guidelines on decision‐making process

Periodontology 2000, Journal Year: 2023, Volume and Issue: 93(1), P. 254 - 269

Published: Oct. 1, 2023

Abstract In the past decades, personalized regenerative medicine has gained increased attention. Autologous platelet concentrates (APCs) such as PRP, PRGF, and L‐PRF, all serving a source of large variety cells growth factors that participate in hard soft tissue healing regeneration, could play significant role periodontal procedures. This narrative review evaluated relative impact APCs alveolar ridge preservation, sinus floor augmentation, regeneration bony craters around teeth, both single substitute or combination with xenograft. L‐PRF beneficial effect on preservation (<alveolar bone resorption, >bone quality). The data for PRGF are less convincing, PRP is controversial. can successfully be used during transcrestal (≥3.5 mm gain) well 1‐stage lateral window elevation (>5 gain). For especially very scarce. treatment open flap debridement, showed adjunctive benefits (e.g., >PPD reduction, >CAL gain, >crater depth reduction). were non‐conclusive. Adding to xenograft OFD resulted additional improvements (>PPD fill), no found. demonstrated enhance most convincing. also advantage greater simplicity production, its 100% autologous character.

Language: Английский

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Platelet-Rich Fibrin Decreases the Inflammatory Response of Mesenchymal Cells

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(21), P. 11333 - 11333

Published: Oct. 20, 2021

Chronic inflammation is a pathological process where cells of the mesenchymal lineage become major source inflammatory mediators. Platelet-rich fibrin (PRF) has been shown to possess potent anti-inflammatory activity in macrophages, but its impact on not investigated. The aim this study was, therefore, expose cytokines together with lysates generated from liquid platelet-poor plasma (PPP), cell-rich buffy coat layer (BC; concentrated-PRF or C-PRF), and remaining red clot (RC), following centrifugation blood. Heating PPP generates an albumin gel (Alb-gel) that when mixed back C-PRF produces Alb-PRF. Membranes prepared solid PRF were also subjected lysis. We report here PPP, BC, decreased cytokine-induced expression interleukin 6 (IL6) nitric oxide synthase (iNOS) bone marrow-derived ST2 cells. Consistently, greatly phosphorylation nuclear translocation p65 response caused by Pam3CSK4 was reduced accordingly. Moreover, enhanced mediators IL6 iNOS 3T3-L1 pre-adipocyte cells, CCL5 murine calvarial Surprisingly, effective reducing human gingival fibroblasts HSC2 epithelial data present suggest both exert

Language: Английский

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Titanium platelet–rich fibrin (T-PRF) as high-capacity doxycycline delivery system

Clinical Oral Investigations, Journal Year: 2022, Volume and Issue: 26(8), P. 5429 - 5438

Published: May 3, 2022

Language: Английский

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