Perspective Chapter: Molecular Diagnostics in Viral Outbreak Surveillance DOI
Jennifer Giandhari, Amsha Viraragavan, Michelle Gordon

и другие.

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

Understanding and adopting various methods for monitoring viral outbreaks is required pathogen surveillance. Accurate diagnosis can play a significant role in the safe effective treatment prescribed. Africa remains burdened with host of infectious diseases, which challenges healthcare systems proper management diseases. Surveillance are implemented some parts that have infrastructure funding to perform routine testing screening. However, not all this continent equipped necessary tools support. With travel, tourism goods exchange, diseases spread across borders rapidly, posing threat global health, emphasising unified need efforts prevent, detect, act on disease through improved access vaccinations treatments. Effective response depends several elements, including laboratory capacity, skilled health staff, surveillance detect limit illnesses rapidly. Traditional molecular such as genotyping polymerase chain reaction (PCR)-based detection now being complemented like next-generation sequencing clustered regularly interspaced short palindromic repeats (CRISPR). This book chapter aims summarise current broadly describes issue climate change its impact outbreaks.

Язык: Английский

CRISPR in clinical diagnostics: bridging the gap between research and practice DOI
Zainab Lafi,

Tha’er Ata,

S. Asha

и другие.

Bioanalysis, Год журнала: 2025, Номер unknown, С. 1 - 10

Опубликована: Фев. 4, 2025

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has transformed molecular biology through its precise gene-editing capabilities. Beyond initial applications in genetic modification, emerged as a powerful tool diagnostics and biosensing. This review explores transition from genome editing to innovative detection methods, including nucleic acid identification, single nucleotide polymorphism (SNP) analysis, protein sensing. Advanced technologies such SHERLOCK DETECTR demonstrate CRISPR's potential for point-of-care diagnostics, enabling rapid highly sensitive detection. The integration of chemical modifications, CRISPR-Chip technology, enzymatic systems like Cas12a Cas13a enhances signal amplification efficiency. These advancements promise decentralized, real-time diagnostic solutions with significant implications global healthcare. Furthermore, the fusion artificial intelligence digital health platforms is paving way more accessible, cost-effective, scalable approaches, ultimately revolutionizing precision medicine.

Язык: Английский

Процитировано

1
CRISPR integrated biosensors: A new paradigm for cancer detection DOI

Ashok Saini,

Neeraj Dilbaghi, Neelam Yadav

и другие.

Clinica Chimica Acta, Год журнала: 2025, Номер 569, С. 120179 - 120179

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1
Rapid Detection of Pratylenchus coffeae Using Recombinase Polymerase Amplification Assay with Lateral Flow Dipsticks DOI
Caiyun Wu, Xueliang Xu,

Linjuan Fan

и другие.

Physiological and Molecular Plant Pathology, Год журнала: 2025, Номер unknown, С. 102608 - 102608

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Current trends in application of CRISPR/Cas9 in gene editing and diagnostics in Neglected tropical diseases (NTDs) DOI

Amneet Kaur Badwal,

Sushma Singh

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Фев. 21, 2025

Язык: Английский

Процитировано

0
Mathematical modeling of a MoSe₂-based SPR biosensor for detecting SARS-CoV-2 at nM concentrations DOI Creative Commons
Talía Tene,

Nataly Bonilla García,

Jéssica Alexandra Marcatoma Tixi

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Фев. 28, 2025

The rapid and accurate detection of SARS-CoV-2 remains a critical challenge in biosensing technology, necessitating the development highly sensitive selective platforms. In this study, we present mathematical modeling approach to optimize MoSe₂-based Surface Plasmon Resonance (SPR) biosensor for detecting novel coronavirus at nM scale. Using Transfer Matrix Method (TMM), systematically biosensor’s structural parameters, including silver (Ag), silicon nitride (Si₃N₄), molybdenum diselenide (MoSe₂), thiol-tethered single-stranded DNA (ssDNA) layers, enhance sensitivity, accuracy, optical performance. results indicate that an optimized 45 nm Ag layer, 10 Si₃N₄ monolayer MoSe₂ configuration achieves resonance shift (Δθ) 0.3° 100 nM, with sensitivity 197.70°/RIU accuracy 5.24 × 10⁻ 2 . Additionally, incorporation ssDNA functionalization layer significantly enhances molecular recognition, lowering limit (LoD) 2.53 5 improving overall efficiency. Sys₅ (MoSe₂ + ssDNA) outperforms Sys₄ without terms specificity reliability, making it more suitable practical applications. These findings establish SPR as promising candidate detection, offering balance between high stability, selectivity, crucial effective viral diagnostics.

Язык: Английский

Процитировано

0
Revolutionizing healthcare: the role of CRISPR-Cas systems in precision medicine DOI Creative Commons
Kirolos Eskandar

Pharmacogenetics and Pharmacogenomics, Год журнала: 2025, Номер 2, С. 29 - 36

Опубликована: Март 4, 2025

Introduction. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has been recognized as a revolutionary advancement in the biomedical field, offering unparalleled precision and versatility genome editing. This review examines transformative potential of diagnostic therapeutic tool for various diseases. Methods. A systematic was conducted following PRISMA (Preferred Reporting Items Systematic Reviews Meta-Analyses) guidelines. comprehensive search PubMed, Scopus, Google Scholar, Web Science performed to identify relevant Studies published between January 2015 2025. The inclusion criteria focused on peer-reviewed articles discussing CRISPR-based diagnostics, applications, technological advancements. were screened, assessed quality using CASP framework, categorized into thematic areas analysis. Results. platforms, such SHERLOCK DETECTR, analyzed their sensitivity rapidity detecting pathogens, cancer biomarkers, genetic mutations. Emerging innovations, including prime base editing, have explored role expanding capabilities CRISPR. Additionally, advancements delivery mechanisms use alternative Cas proteins discussed impact clinical applicability. Conclusions. Ethical, regulatory, accessibility challenges associated with technology are highlighted, emphasizing importance responsible development equitable deployment. connects cutting-edge translational underscores significant shaping future medicine global health.

Язык: Английский

Процитировано

0
Recent advances in DNA nanotechnology for cancer detection and therapy: A review DOI

Donya Esmaeilpour,

Matineh Ghomi, Ehsan Nazarzadeh Zare‬

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142136 - 142136

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Perspective Chapter: Molecular Diagnostics in Viral Outbreak Surveillance DOI
Jennifer Giandhari, Amsha Viraragavan, Michelle Gordon

и другие.

IntechOpen eBooks, Год журнала: 2025, Номер unknown

Опубликована: Апрель 16, 2025

Understanding and adopting various methods for monitoring viral outbreaks is required pathogen surveillance. Accurate diagnosis can play a significant role in the safe effective treatment prescribed. Africa remains burdened with host of infectious diseases, which challenges healthcare systems proper management diseases. Surveillance are implemented some parts that have infrastructure funding to perform routine testing screening. However, not all this continent equipped necessary tools support. With travel, tourism goods exchange, diseases spread across borders rapidly, posing threat global health, emphasising unified need efforts prevent, detect, act on disease through improved access vaccinations treatments. Effective response depends several elements, including laboratory capacity, skilled health staff, surveillance detect limit illnesses rapidly. Traditional molecular such as genotyping polymerase chain reaction (PCR)-based detection now being complemented like next-generation sequencing clustered regularly interspaced short palindromic repeats (CRISPR). This book chapter aims summarise current broadly describes issue climate change its impact outbreaks.

Язык: Английский

Процитировано

0