Physiological and Molecular Plant Pathology, Год журнала: 2024, Номер 134, С. 102451 - 102451
Опубликована: Окт. 22, 2024
Язык: Английский
Physiological and Molecular Plant Pathology, Год журнала: 2024, Номер 134, С. 102451 - 102451
Опубликована: Окт. 22, 2024
Язык: Английский
Опубликована: Янв. 1, 2025
Процитировано
0Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(43), С. 23937 - 23946
Опубликована: Окт. 11, 2024
Cascade conversion of chitin into soluble and functional chitooligosaccharides has gained great attention. However, the biotransformation route is still limited to low catalytic performances deacetylases (CDAs) complicated procedures. In this study, a CDA from Arthrobacter sp. Jub115 (ArCDA) was identified characterized, which showed higher stability than reported CDAs, with residual activity 80.49%, 71.12%, 56.09% after incubation at 30, 35, 40 °C for 24 h, respectively. Additionally, ArCDA have broad substrate spectrum toward β-chitin N-acetyl chitooligosaccharides. Moreover, an engineered chitin-degrading bacteria (CDB) cell-surface-displayed deacetylase chitinase SaChiB constructed simplify catalysis procedures, facilitating chitobiose production 294.30 ± 16.43 mg/L in 10 h. This study not only desirable performance but also provided strategy constructing CDB, high-value utilization chitin.
Язык: Английский
Процитировано
2Physiological and Molecular Plant Pathology, Год журнала: 2024, Номер 134, С. 102451 - 102451
Опубликована: Окт. 22, 2024
Язык: Английский
Процитировано
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