Physiological and Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 134, P. 102451 - 102451
Published: Oct. 22, 2024
Language: Английский
Physiological and Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 134, P. 102451 - 102451
Published: Oct. 22, 2024
Language: Английский
Published: Jan. 1, 2025
Citations
0Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(43), P. 23937 - 23946
Published: Oct. 11, 2024
Cascade conversion of chitin into soluble and functional chitooligosaccharides has gained great attention. However, the biotransformation route is still limited to low catalytic performances deacetylases (CDAs) complicated procedures. In this study, a CDA from Arthrobacter sp. Jub115 (ArCDA) was identified characterized, which showed higher stability than reported CDAs, with residual activity 80.49%, 71.12%, 56.09% after incubation at 30, 35, 40 °C for 24 h, respectively. Additionally, ArCDA have broad substrate spectrum toward β-chitin N-acetyl chitooligosaccharides. Moreover, an engineered chitin-degrading bacteria (CDB) cell-surface-displayed deacetylase chitinase SaChiB constructed simplify catalysis procedures, facilitating chitobiose production 294.30 ± 16.43 mg/L in 10 h. This study not only desirable performance but also provided strategy constructing CDB, high-value utilization chitin.
Language: Английский
Citations
2Physiological and Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 134, P. 102451 - 102451
Published: Oct. 22, 2024
Language: Английский
Citations
0