Xanthomonas

Published: Jan. 1, 2025

Chitinase and Deacetylase-Based Chitin-Degrading Bacteria: One-Pot Cascade Bioconversion of Chitin to Chitooligosaccharides

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(43), P. 23937 - 23946

Published: Oct. 11, 2024

Cascade conversion of chitin into soluble and functional chitooligosaccharides has gained great attention. However, the biotransformation route is still limited to low catalytic performances deacetylases (CDAs) complicated procedures. In this study, a CDA from Arthrobacter sp. Jub115 (ArCDA) was identified characterized, which showed higher stability than reported CDAs, with residual activity 80.49%, 71.12%, 56.09% after incubation at 30, 35, 40 °C for 24 h, respectively. Additionally, ArCDA have broad substrate spectrum toward β-chitin N-acetyl chitooligosaccharides. Moreover, an engineered chitin-degrading bacteria (CDB) cell-surface-displayed deacetylase chitinase SaChiB constructed simplify catalysis procedures, facilitating chitobiose production 294.30 ± 16.43 mg/L in 10 h. This study not only desirable performance but also provided strategy constructing CDB, high-value utilization chitin.

Language: Английский

Production of Chitinase in Elicited Tomato Cell Suspensions and Its Application as a Biopesticide and Fungicide against Soil-borne Pests and Fungi

Physiological and Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 134, P. 102451 - 102451

Published: Oct. 22, 2024

Language: Английский