Development of a Novel Prognostic Model for Lung Adenocarcinoma Utilizing Pyroptosis‐Associated LncRNAs

Analytical Cellular Pathology, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

Lung cancer is a highly prevalent and fatal that seriously threatens the safety of people in various regions around world. Difficulty early diagnosis strong drug resistance have always been difficulties treatment lung cancer, so prognosis has focus scientific researchers. This study used genotype-tissue expression (GTEx) genome atlas (TCGA) databases to obtain 477 adenocarcinoma (LUAD) 347 healthy individuals' samples as research subjects divided LUAD patients into low-risk high-risk groups based on prognostic risk scores. Differentially expressed gene (DEG) analysis was performed 25 pyroptosis-related genes obtained from GeneCards MSigDB tissues noncancerous individuals, seven were significantly different among them. Coexpression differential these long noncoding RNAs (lncRNAs) found three lncRNAs (AC012615.1, AC099850.3, AO0001453.2) had significant differences between tissues. We Cox regression least absolute shrinkage sum selection operator (LASSO) construct model for with (pRLs) analyzed value pRLs by Likaplan-Meier curve regression. The results show prediction good ability. In addition, we also studied tumor mutation burden (TMB), immune dysfunction rejection (TIDE), microenvironment scores groups. successfully established pRLs, which provides new insights lncRNA-based strategies.

Язык: Английский

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Cyclometalated iridium(III)-lonidamine conjugates: Mitochondrial targeting and pyroptosis induction

Journal of Inorganic Biochemistry, Год журнала: 2025, Номер 266, С. 112852 - 112852

Опубликована: Фев. 9, 2025

Язык: Английский

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Overview of pyroptosis mechanism and in-depth analysis of cardiomyocyte pyroptosis mediated by NF-κB pathway in heart failure

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 179, С. 117367 - 117367

Опубликована: Авг. 29, 2024

The pyroptosis of cardiomyocytes has become an essential topic in heart failure research. abnormal accumulation these biological factors, including angiotensin II, advanced glycation end products, and various growth factors (such as connective tissue factor, vascular endothelial transforming factor beta, among others), activates the nuclear factor-κB (NF-κB) signaling pathway cardiovascular diseases, ultimately leading to cardiomyocytes. Therefore, exploring underlying molecular mechanisms is for developing novel drugs therapeutic strategies. However, our current understanding precise regulatory mechanism this complex cardiomyocyte still limited. Given this, study reviews milestone discoveries field research since 1986, analyzes detail similarities, differences, interactions between other cell death modes apoptosis, necroptosis, autophagy, ferroptosis), explores deep connection failure. At same time, it depicts complete activation, transmission, eventual NF-κB process In addition, also systematically summarizes approaches that can inhibit reduce pyroptosis, drugs, natural compounds, small molecule inhibitors, gene editing, cutting-edge technologies, aiming provide solid scientific support new perspectives prevention treatment

Язык: Английский

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Advancements in the Alcohol-Associated Liver Disease Model

Biomolecules, Год журнала: 2022, Номер 12(8), С. 1035 - 1035

Опубликована: Июль 27, 2022

Alcohol-associated liver disease (ALD) is an intricate that results in a broad spectrum of damage. The presentation ALD can include simple steatosis, steatohepatitis, fibrosis, cirrhosis, and even hepatocellular carcinoma (HCC). Effective prevention treatment strategies are urgently required for patients. In previous decades, numerous rodent models were established to investigate the mechanisms alcohol-associated explore therapeutic targets. This review provides summary latest developments models, including those involve EtOH administration, which will help us understand characteristics causes at different stages. addition, we discuss pathogenesis summarize existing vitro models. We analyse pros cons these their translational relevance insights have been gained regarding alcoholic injury.

Язык: Английский

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Imaging of cell death in malignancy: Targeting pathways or phenotypes?

Nuclear Medicine and Biology, Год журнала: 2023, Номер 124-125, С. 108380 - 108380

Опубликована: Авг. 13, 2023

Cell death is fundamental in health and disease resisting cell a hallmark of cancer. Treatment malignancy aims to cause cancer death, however current clinical imaging treatment response does not specifically image but assesses this indirectly either by changes tumor size (using x-ray computed tomography) or metabolic activity 2-[18F]fluoro-2-deoxy-glucose positron emission tomography). The ability directly soon after commencement therapy would enable personalised adapted approaches that presently possible with imaging, which many circumstances neither sufficiently accurate nor timely. Several pathways have now been identified characterised present multiple potential targets for including externalisation phosphatidylserine phosphatidylethanolamine, caspase activation La autoantigen redistribution. However, targeting one specific pathway carries the risk detecting other it understood induces different sometimes pathways. An alternative approach phenotype "agnostic" pathway. phenotypes targeted include loss plasma membrane integrity dissipation mitochondrial potential. Targeting may advantage being more sensitive generalisable imaging. This review describes summarises radiopharmaceuticals investigated phenotype.

Язык: Английский

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Roles and Mechanisms of Regulated Necrosis in Corneal Diseases: Progress and Perspectives

Journal of Ophthalmology, Год журнала: 2022, Номер 2022, С. 1 - 11

Опубликована: Май 23, 2022

Regulated necrosis is defined as cell death characterized by loss of the membrane integrity and release cytoplasmic content. It contributes to development progression some diseases, including ischemic stroke injury, liver hypertension, cancer. Various forms regulated necrosis, particularly pyroptosis, necroptosis, ferroptosis, have been implicated in pathogenesis corneal disease. cells enhances inflammatory reactions adjacent tissues, leading recurrence aggravation In this review, we summarize molecular mechanisms ferroptosis diseases discuss roles inflammation regulation, tissue repair, disease outcomes.

Язык: Английский

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A novel pyroptosis scoring model was associated with the prognosis and immune microenvironment of esophageal squamous cell carcinoma

Frontiers in Genetics, Год журнала: 2023, Номер 13

Опубликована: Янв. 4, 2023

The phenotype of pyroptosis has been extensively studied in a variety tumors, but the relationship between and esophageal squamous cell carcinoma (ESCC) remains unclear. Here, 22 genes were downloaded from website Gene Set Enrichment Analysis (GSEA), 79 samples GSE53625 containing 179 pairs collected Cancer Genome Atlas (TCGA) Expression Omnibus (GEO), respectively. Then, subtypes obtained by cluster analysis according to expression difference genes, scoring model was constructed pyroptosis-related screened different subtypes. Time-dependent receiver operator characteristic (timeROC) curves area under curve (AUC) values used evaluate prognostic predictive accuracy model. Kaplan-Meier method with log-rank test conducted analyze impact on overall survival (OS) patients carcinoma. Nomogram models calibration further confirm effect prognosis. Meanwhile, CIBERSORTx ESTIMATE algorithm applied calculate influence immune microenvironment. Our findings revealed that established associated prognosis microenvironment carcinoma, which can be as biomarker predict act potential target for treatment

Язык: Английский

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Pyroptosis Modulators: New Insights of Gasdermins in Health and Disease

Antioxidants, Год журнала: 2023, Номер 12(8), С. 1551 - 1551

Опубликована: Авг. 3, 2023

Pyroptosis is an inflammation-dependent type of cell death that has been in the spotlight for scientific community last few years. Crucial players process pyroptosis are members gasdermin family proteins, which have parallelly studied. Upon induction pyroptosis, gasdermins suffer from structural changes leading to formation pores membrane subsequently cause release pro-inflammatory contents. Recently, it discovered oxidation plays a key role activation certain gasdermins. Here, we review current knowledge on and human gasdermins, focusing description different family, their molecular structures, influence health disease directly or non-directly related inflammation. Noteworthy, focused existing understanding this proteins cancer, could translate into novel promising strategies aimed at benefiting health. In conclusion, modulation by natural synthetic compounds through mechanisms, including modification redox state cells, proven effective sets precedents future therapeutic strategies.

Язык: Английский

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Genomic and Immunological Characterization of Pyroptosis in Lung Adenocarcinoma

Journal of Oncology, Год журнала: 2022, Номер 2022, С. 1 - 20

Опубликована: Июль 27, 2022

Pyroptosis is a programmed cell death that may either promote or hinder cancer growth under different circumstances. Pyroptosis-related genes (PRGs) could be useful target for therapy, and are uncommon in lung adenocarcinoma (LUAD). The expression profiles, mutation data clinical information of LUAD patients were included this study. A pyroptosis-related prognostic risk score (PPRS) model was constructed by performing Cox regression, weighted gene co-expression network analysis (WGCNA), least absolute shrinkage selection operator (LASSO) to patients. Somatic copy number variation (CNV), tumor immunity, sensitivity immunotherapy/chemotherapy compared between PPRS groups. Clinical parameters combined with construct decision tree nomogram. Red module highly positively correlated pyroptosis. Seven (FCRLB, COTL1, GNG10, CASP4, DOK1, CCR2, AQP8) screened from the red model. Significantly lower overall survival (OS), higher incidence somatic CNV, elevated infiltration level immune together increased probability escape observed PPRS, more sensitive Cisplatin, Docetaxel, Vinorelbine. We new LUAD. might have significance prediction prognosis efficacy chemotherapy immunotherapy.

Язык: Английский

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Okanin Inhibits Cell Growth and Induces Apoptosis and Pyroptosis in Oral Cancer

Cancers, Год журнала: 2024, Номер 16(18), С. 3195 - 3195

Опубликована: Сен. 19, 2024

Background: Okanin, a flavonoid compound derived from Bidens pilosa L., has garnered attention for its anti-inflammatory properties. Although is commonly used in healthcare products and functional foods, the anticancer potential of okanin, particularly oral cancer, remains underexplored. This study aims to investigate effects okanin on cancer cell lines as therapeutic agent. Methods: The involved assessing cytotoxic SAS, SCC25, HSC3, OEC-M1. IC50 values were determined using methylene blue assays, clonogenic capacity was evaluated through colony formation assays. Flow cytometry analyze cycle progression apoptosis. Caspase-3/7 activity assays annexin V/7-AAD staining confirmed induction apoptosis pyroptosis. In vivo efficacy assessed SAS xenograft model, immunohistochemical analysis tissue performed examine pyroptosis-related markers. Results: Okanin exhibited potent with 12.0 ± 0.8, 58.9 18.7, 18.1 5.3, 43.2 6.2 μM OEC-M1 cells, respectively. It caused dose- time-dependent reductions viability significantly impaired capacity. revealed G2/M arrest increased sub-G1 population, indicating disruption death. induced both pyroptosis, by caspase-3/7 staining. vivo, reduced tumor growth markers such CASP1, GSDMC, GSDMD, GSDME. Conclusions: demonstrates significant potential, inducing Its reducing further supports novel option. Further mechanistic studies are needed elucidate pathways okanin-mediated death explore clinical applications.

Язык: Английский

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