Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Ноя. 20, 2024
Abstract
Viral
diseases
arising
in
farmed
fish
are
an
ongoing
challenge
to
the
aquaculture
industry,
causing
severe
mortality
and
economic
losses.
Recently,
there
has
been
a
spike
incidence
of
viral
disease
caused
by
Tilapia
Parvovirus
(TiPV)
inflicts
irreparable
damage,
large-scale
kills
tilapia
species.
We
investigated
case
outbreak
cage
wetland
farms
West
Bengal
Odisha,
India.
The
symptomatic
showed
clinical
signs,
including
hemorrhage,
discoloration,
ulcer,
redness
body
surfaces.
Further
analysis
revealed
that
was
associated
(validated
PCR,
phylogenetic
analysis,
cell
line
assay)
with
infection
tilapia.
virus
detected
gill,
heart,
spleen,
liver,
kidney
samples
collected
from
apparently
healthy
(asymptomatic)
farms.
At
same
time,
negative
results
were
found
brain
skin
tissue
samples.
histological
TiPV
induces
damage
invariably
almost
all
studied
tissue,
kidney,
quantification
genome
higher
heart
than
samples’
or
tissue.
Furthermore,
study
indicated
significant
effect
on
health
exhibited
immune
reactivity
toward
(upregulation
chemokine
receptors,
CRs
interleukin
1β,
IL-1β
),
majority
genes
(interleukin
8,
IL-8
;
Toll-like
receptors
7,
TLR7
tumour
necrosis
factor
α,
TNF-α
major
histocompatibility
complex
II,
MHC
II
nuclear
kappa
B,
NF-kB
)
significantly
downregulated
spleen
liver
Further,
vivo
assay
confirms
isolated
is
novel
parvovirus
pathogen
causes
massive
lessons
learned
first
cellular
molecular
description
epidemiology
could
be
critical
developing
current
state
farming
industry.
Additionally,
holistic
approach
needed
develop
management
measures
control
virulence
risk
factors
TiPV.
PeerJ,
Год журнала:
2025,
Номер
13, С. e19425 - e19425
Опубликована: Май 13, 2025
Tilapia
aquaculture
faces
significant
threats
posed
by
four
prominent
pathogens:
tilapia
lake
virus
(TiLV),
infectious
spleen
and
kidney
necrosis
(ISKNV),
Francisella
orientalis,
Streptococcus
agalactiae.
Currently,
employed
molecular
diagnostic
methods
for
these
pathogens
rely
on
multiple
singleplex
polymerase
chain
reactions
(PCR),
which
are
time-consuming
expensive.
In
this
study,
we
present
an
approach
utilizing
a
multiplex
PCR
(mPCR)
assay,
coupled
with
rapid
Nanopore
sequencing,
enabling
the
one-tube
simultaneous
detection
one-reaction
sequencing-based
validation
of
pathogens.
Our
assay
exhibits
limit
1,000
copies
per
reaction
TiLV,
ISKNV,
S.
agalactiae,
while
F.
is
10,000
reaction.
This
sensitivity
sufficient
diagnosing
infections
co-infections
in
clinical
samples
from
sick
fish,
confirmation
presence
Integrating
sequencing
provides
alternative
platform
fast
precise
diagnostics
major
clinically
animals,
adding
to
available
toolbox
disease
diagnostics.
International Journal of Microbiology,
Год журнала:
2025,
Номер
2025(1)
Опубликована: Янв. 1, 2025
The
catfish
is
a
prominent
freshwater
fish
species
farmed
in
Cameroon
to
meet
the
escalating
demand
for
products.
Despite
considerable
growth
potential,
there
are
concerns
about
occurrence
of
bacteria
pathogenic
both
and
humans
within
aquaculture
systems.
Research
on
microbiome
their
habitats
remains
largely
unexplored.
Given
critical
importance
understanding
microbial
composition
systems
ensure
food
safety
protect
public
health,
this
study
aimed
generate
vital
preliminary
data
by
investigating
bacteriome
gills
intestines
pond
water
environment
using
16S
rRNA
gene
amplicon
sequencing.
findings
revealed
diverse
bacterial
community
(30
phyla,
678
genera,
1056
species),
with
Fusobacteria,
Bacteroidetes,
Proteobacteria,
Firmicutes,
Verrucomicrobia
collectively
representing
over
93%
observed.
Notably,
Fusobacteria
emerged
as
dominant
phylum
(49.98%)
(65.3%),
while
Proteobacteria
predominated
(40.24%).
Bacteria
genus
Cetobacterium
dominated
all
three
samples
(gills,
49.93%;
intestines,
65.19%;
water,
23.85%).
Furthermore,
identified
many
including
potential
pathogens
such
Edwardsiella
,
Aeromonas
Plesiomonas
Flavobacterium
human
gut
Clostridium
Bacteroides
alongside
beneficial
probiotic
Lactococcus
spp.
coexistence
potentially
underscores
ecological
complex
dynamics
highlights
need
thorough
management
strategies.
This
provides
insights
into
landscape
Cameroonian
aquaculture,
revealing
risks
benefits
farming.
Aquaculture and Fisheries,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 1, 2024
Tilapia
aquaculture
is
a
commercially
important
and
lucrative
trade
with
global
production
predicted
to
increase
in
the
future.
Owing
robust
disease-resistant
nature
of
tilapia,
tilapia
has
expanded
intensified
over
years.
However,
intensification
led
emergence
diseases,
whereby
viral
diseases
represent
prominent
limiting
factor
growth
aquaculture.
This
review
aims
discuss
all
infections
emphasis
on
aetiology,
occurrence,
transmission,
pathology,
diagnostic
methods,
control
measures
mitigate
outbreaks
To
date,
ten
infectious
have
been
reported
globally
various
species
reared
or
laboratory
settings.
our
knowledge,
this
article
represents
first
instance
reviewing
parvovirus
(TiPV)
infection
addition
larvae
encephalitis
virus
(TLEV),
spleen
kidney
necrosis
(ISKNV),
iridovirus-like
agent,
novel
megalocytivirus,
Bohle
iridovirus
(BIV),
lymphocystis
disease
(LCDV),
pancreatic
(IPNV),
nervous
(NNV),
lake
(TiLV).
Marine and Fishery Sciences (MAFIS),
Год журнала:
2024,
Номер
37(2)
Опубликована: Фев. 19, 2024
Despite
all
the
technical-scientific
knowledge
about
streptococcosis,
high
prevalence
of
bacterial
infections
caused
by
Streptococcus
spp.
in
Nile
tilapia
farming
implies
frequent,
often
irresponsible,
use
antibiotics.
The
chemotherapy
aquaculture
environments
remains
an
efficient
practice
treatment
and
disease
prevention.
Research
have
shown
that
emergence
antimicrobial
resistance
(AMR)
farmed
fish
is
one
main
challenges
faced
aquaculture.
It
known
emerging
AMR
can
be
transferred
to
clinically
important
strains
from
natural
environment
through
horizontal
gene
transfer
(HGT)
affecting
entire
aquatic
ecosystem.
Maintaining
health
farms
promotes
sustainability
production
systems
and,
consequently,
improves
final
quality
farm
products.
Therefore,
objective
this
review
was
provide
information
on
prevention,
control
eradication
diseases
farms,
how
such
management
plays
a
fundamental
role
maintaining
public
ensuring
adequate
sanitary
conditions
for
animals
intended
human
consumption.
Aquaculture and Fisheries,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 1, 2024
Tilapia
aquaculture
contributes
significantly
to
the
nutritional
requirements
of
humans.
Because
microbes
play
a
pivotal
role
in
maintaining
host's
physiology
and
biochemistry,
research
on
microbiomes
associated
with
tilapia
rearing
pond
water
sediment
can
contribute
increased
production.
We
applied
16S
rRNA
gene
amplicon
sequencing
elucidate
bacterial
community
structures
gills
intestine
Nile
as
well
their
ponds.
identified
total
9770
Operational
Taxonomic
Units
(OTUs)
all
samples.
The
samples,
which
generated
3884
unique
OTUs,
had
highest
level
alpha-diversity
(Shannon
index,
P
<
0.01)
among
Fusobacteria
(59%)
predominated
constituted
indicator
phylum
gills,
while
Proteobacteria
was
prevailed
(52%),
(28%),
(33%).
were
not
much
similar
those
surrounding
sediment,
probably
due
host-specific
microbial
selection.
More
than
70%
flora
beneficial,
whereas
nearly
10%
pathogenic
tilapia.
This
study
aims
explore
microbiome
Bangladesh,
results
suggest
that
communities
are
eubiotic
state,
indicates
healthy
state
for
better
growth
production
aquaculture.
Fishes,
Год журнала:
2024,
Номер
9(10), С. 374 - 374
Опубликована: Сен. 25, 2024
Microalgae
are
promising
as
prebiotics
in
aquaculture.
Arthrospira
maxima
has
potential
nutritional
value
but
is
poorly
studied.
We
assessed
A.
feed
supplementation
tilapia
fry
and
evaluated
its
effect
on
growth
performance
gut
microbiota.
Fish
were
cultivated
ponds
under
0%,
5%,
10%,
15%
inclusion
treatments.
Growth
parameters
biomass
proximate
analysis
assessed.
A
meta-amplicon
was
performed
the
microbiota
using
DADA2
PICRUST2
for
functional
prediction.
Among
treatments,
5%
group
seemed
to
present
no
negative
did
not
compromise
quality
of
biomass.
Microbial
composition
characterized
by
Cetobacterium,
Pseudomonas
Aeromonas
genera,
a
predominance
beneficial
metabolic
pathways.
Microbiota
fed
with
showed
unique
genera
reported
functions
tilapia.
The
abundance
pathogenic
taxa
significantly
decreased
possibly
related
valuable
compounds
maxima.
microalgae
supported
stability
microbiota,
favoring
commensal
species.
This
seems
have
an
decreasing
presence
gastrointestinal
tract.
Overall,
our
work
proposes
be
most
suitable
aquaculture
maintain
production
rates
while
improving
general
health.
