Pathological effects and immune modulation in host during Tilapia Parvovirus (TiPV) outbreak in cage and wetland Tilapia farms DOI Creative Commons
Basanta Kumar Das,

Vikash Kumar,

Suvra Roy

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 20, 2024

Abstract Viral diseases arising in farmed fish are an ongoing challenge to the aquaculture industry, causing severe mortality and economic losses. Recently, there has been a spike incidence of viral disease caused by Tilapia Parvovirus (TiPV) inflicts irreparable damage, large-scale kills tilapia species. We investigated case outbreak cage wetland farms West Bengal Odisha, India. The symptomatic showed clinical signs, including hemorrhage, discoloration, ulcer, redness body surfaces. Further analysis revealed that was associated (validated PCR, phylogenetic analysis, cell line assay) with infection tilapia. virus detected gill, heart, spleen, liver, kidney samples collected from apparently healthy (asymptomatic) farms. At same time, negative results were found brain skin tissue samples. histological TiPV induces damage invariably almost all studied tissue, kidney, quantification genome higher heart than samples’ or tissue. Furthermore, study indicated significant effect on health exhibited immune reactivity toward (upregulation chemokine receptors, CRs interleukin 1β, IL-1β ), majority genes (interleukin 8, IL-8 ; Toll-like receptors 7, TLR7 tumour necrosis factor α, TNF-α major histocompatibility complex II, MHC II nuclear kappa B, NF-kB ) significantly downregulated spleen liver Further, vivo assay confirms isolated is novel parvovirus pathogen causes massive lessons learned first cellular molecular description epidemiology could be critical developing current state farming industry. Additionally, holistic approach needed develop management measures control virulence risk factors TiPV.

Язык: Английский

Multiplex polymerase chain reaction (PCR) with Nanopore sequencing for sequence-based detection of four tilapia pathogens DOI Creative Commons
Jérôme Delamare‐Deboutteville,

Watcharachai Meemetta,

Khaettareeya Pimsannil

и другие.

PeerJ, Год журнала: 2025, Номер 13, С. e19425 - e19425

Опубликована: Май 13, 2025

Tilapia aquaculture faces significant threats posed by four prominent pathogens: tilapia lake virus (TiLV), infectious spleen and kidney necrosis (ISKNV), Francisella orientalis, Streptococcus agalactiae. Currently, employed molecular diagnostic methods for these pathogens rely on multiple singleplex polymerase chain reactions (PCR), which are time-consuming expensive. In this study, we present an approach utilizing a multiplex PCR (mPCR) assay, coupled with rapid Nanopore sequencing, enabling the one-tube simultaneous detection one-reaction sequencing-based validation of pathogens. Our assay exhibits limit 1,000 copies per reaction TiLV, ISKNV, S. agalactiae, while F. is 10,000 reaction. This sensitivity sufficient diagnosing infections co-infections in clinical samples from sick fish, confirmation presence Integrating sequencing provides alternative platform fast precise diagnostics major clinically animals, adding to available toolbox disease diagnostics.

Язык: Английский

Процитировано

0

Bacterial Diversity in Aquacultured African Catfish and Source Pond Water in Buea, Cameroon DOI Creative Commons
Gordon Takop Nchanji, Bertrand Tatsinkou Fossi,

Jerome Fru-Cho

и другие.

International Journal of Microbiology, Год журнала: 2025, Номер 2025(1)

Опубликована: Янв. 1, 2025

The catfish is a prominent freshwater fish species farmed in Cameroon to meet the escalating demand for products. Despite considerable growth potential, there are concerns about occurrence of bacteria pathogenic both and humans within aquaculture systems. Research on microbiome their habitats remains largely unexplored. Given critical importance understanding microbial composition systems ensure food safety protect public health, this study aimed generate vital preliminary data by investigating bacteriome gills intestines pond water environment using 16S rRNA gene amplicon sequencing. findings revealed diverse bacterial community (30 phyla, 678 genera, 1056 species), with Fusobacteria, Bacteroidetes, Proteobacteria, Firmicutes, Verrucomicrobia collectively representing over 93% observed. Notably, Fusobacteria emerged as dominant phylum (49.98%) (65.3%), while Proteobacteria predominated (40.24%). Bacteria genus Cetobacterium dominated all three samples (gills, 49.93%; intestines, 65.19%; water, 23.85%). Furthermore, identified many including potential pathogens such Edwardsiella , Aeromonas Plesiomonas Flavobacterium human gut Clostridium Bacteroides alongside beneficial probiotic Lactococcus spp. coexistence potentially underscores ecological complex dynamics highlights need thorough management strategies. This provides insights into landscape Cameroonian aquaculture, revealing risks benefits farming.

Язык: Английский

Процитировано

0

A systematic review on the development and efficacy of nano-based vaccine in aquaculture DOI
Saira Ishaq, Muhammad Salahudin Kheirel Anuar, Abdulwakil Olawale Saba

и другие.

Aquaculture International, Год журнала: 2025, Номер 33(5)

Опубликована: Май 27, 2025

Язык: Английский

Процитировано

0

Current updates on viral infections affecting tilapia DOI Creative Commons

Christal Winona Clyde,

Jin Ping Tan,

Swee Keong Yeap

и другие.

Aquaculture and Fisheries, Год журнала: 2024, Номер unknown

Опубликована: Июль 1, 2024

Tilapia aquaculture is a commercially important and lucrative trade with global production predicted to increase in the future. Owing robust disease-resistant nature of tilapia, tilapia has expanded intensified over years. However, intensification led emergence diseases, whereby viral diseases represent prominent limiting factor growth aquaculture. This review aims discuss all infections emphasis on aetiology, occurrence, transmission, pathology, diagnostic methods, control measures mitigate outbreaks To date, ten infectious have been reported globally various species reared or laboratory settings. our knowledge, this article represents first instance reviewing parvovirus (TiPV) infection addition larvae encephalitis virus (TLEV), spleen kidney necrosis (ISKNV), iridovirus-like agent, novel megalocytivirus, Bohle iridovirus (BIV), lymphocystis disease (LCDV), pancreatic (IPNV), nervous (NNV), lake (TiLV).

Язык: Английский

Процитировано

3

Enhancing growth, immunity, and gene expression in Nile Tilapia (Oreochromis niloticus) through dietary supplementation with avocado (Persea americana) seed powder DOI Creative Commons
Chinh Le Xuan, Nguyen Vu Linh,

Supreya Wannavijit

и другие.

Aquaculture Reports, Год журнала: 2024, Номер 39, С. 102432 - 102432

Опубликована: Окт. 17, 2024

Язык: Английский

Процитировано

2

DF-DETR: Dead fish-detection transformer in recirculating aquaculture system DOI
Tao Fu, Dejun Feng, Pingchuan Ma

и другие.

Aquaculture International, Год журнала: 2024, Номер 33(1)

Опубликована: Ноя. 13, 2024

Язык: Английский

Процитировано

2

Antimicrobial resistance: a concern related to streptococcosis in tilapia farming DOI Creative Commons
Marco Shizuo Owatari, José Luiz Pedreira Mouriño, Maurício Laterça Martins

и другие.

Marine and Fishery Sciences (MAFIS), Год журнала: 2024, Номер 37(2)

Опубликована: Фев. 19, 2024

Despite all the technical-scientific knowledge about streptococcosis, high prevalence of bacterial infections caused by Streptococcus spp. in Nile tilapia farming implies frequent, often irresponsible, use antibiotics. The chemotherapy aquaculture environments remains an efficient practice treatment and disease prevention. Research have shown that emergence antimicrobial resistance (AMR) farmed fish is one main challenges faced aquaculture. It known emerging AMR can be transferred to clinically important strains from natural environment through horizontal gene transfer (HGT) affecting entire aquatic ecosystem. Maintaining health farms promotes sustainability production systems and, consequently, improves final quality farm products. Therefore, objective this review was provide information on prevention, control eradication diseases farms, how such management plays a fundamental role maintaining public ensuring adequate sanitary conditions for animals intended human consumption.

Язык: Английский

Процитировано

1

Heterogeneous bacterial communities in gills and intestine of Nile tilapia (Oreochromis niloticus) and in water and sediments of aquaculture ponds in Bangladesh DOI Creative Commons
Anwar Hossain,

Md. Al Zahid,

Santonu Kumar Sanyal

и другие.

Aquaculture and Fisheries, Год журнала: 2024, Номер unknown

Опубликована: Июль 1, 2024

Tilapia aquaculture contributes significantly to the nutritional requirements of humans. Because microbes play a pivotal role in maintaining host's physiology and biochemistry, research on microbiomes associated with tilapia rearing pond water sediment can contribute increased production. We applied 16S rRNA gene amplicon sequencing elucidate bacterial community structures gills intestine Nile as well their ponds. identified total 9770 Operational Taxonomic Units (OTUs) all samples. The samples, which generated 3884 unique OTUs, had highest level alpha-diversity (Shannon index, P < 0.01) among Fusobacteria (59%) predominated constituted indicator phylum gills, while Proteobacteria was prevailed (52%), (28%), (33%). were not much similar those surrounding sediment, probably due host-specific microbial selection. More than 70% flora beneficial, whereas nearly 10% pathogenic tilapia. This study aims explore microbiome Bangladesh, results suggest that communities are eubiotic state, indicates healthy state for better growth production aquaculture.

Язык: Английский

Процитировано

1

Impact of Arthrospira maxima Feed Supplementation on Gut Microbiota and Growth Performance of Tilapia Fry (Oreochromis niloticus) DOI Creative Commons
Dorian Rojas-Villalta, Olman Gómez-Espinoza, Rossy Guillén-Watson

и другие.

Fishes, Год журнала: 2024, Номер 9(10), С. 374 - 374

Опубликована: Сен. 25, 2024

Microalgae are promising as prebiotics in aquaculture. Arthrospira maxima has potential nutritional value but is poorly studied. We assessed A. feed supplementation tilapia fry and evaluated its effect on growth performance gut microbiota. Fish were cultivated ponds under 0%, 5%, 10%, 15% inclusion treatments. Growth parameters biomass proximate analysis assessed. A meta-amplicon was performed the microbiota using DADA2 PICRUST2 for functional prediction. Among treatments, 5% group seemed to present no negative did not compromise quality of biomass. Microbial composition characterized by Cetobacterium, Pseudomonas Aeromonas genera, a predominance beneficial metabolic pathways. Microbiota fed with showed unique genera reported functions tilapia. The abundance pathogenic taxa significantly decreased possibly related valuable compounds maxima. microalgae supported stability microbiota, favoring commensal species. This seems have an decreasing presence gastrointestinal tract. Overall, our work proposes be most suitable aquaculture maintain production rates while improving general health.

Язык: Английский

Процитировано

1

Evaluation of a Low-Temperature Immersion Immunization Strategy for the Infectious Spleen and Kidney Necrosis Virus orf037l Gene-Deleted Attenuated Vaccine DOI Creative Commons
Weiqiang Pan,

Jiajie Fu,

Ruoyun Zeng

и другие.

Vaccines, Год журнала: 2024, Номер 12(10), С. 1170 - 1170

Опубликована: Окт. 14, 2024

Infectious spleen and kidney necrosis virus (ISKNV) poses a significant threat to aquaculture sustainability, particularly affecting mandarin fish (

Язык: Английский

Процитировано

1