Characterization of pesticidal crystal toxin protein Cry11Aa from Bacillus thuringiensis serovar israelensis VCRC-B646 for mosquito control.
V Abhisubesh,
Sahadiya Mandodan,
Jibi Lukose
и другие.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 24, 2025
Abstract
Effective
mosquito
control
is
essential
for
reducing
the
transmission
of
vector-borne
diseases.
This
study
focuses
on
comprehensive
characterization
mosquitocidal
toxins
produced
by
Bacillus
thuringiensis
serovar
israelensis
(Bti)
VCRC
B646
and
associated
insecticidal
genes.
The
bacterium
was
cultured,
spore-crystal
complex
purified
to
identify
proteins.
isolate
were
effective
against
Aedes
aegypti,
Anopheles
stephensi,
Culex
quinquefasciatus,
Toxicity
bioassays
indicated
lethal
concentrations
(LC
50
LC
90)
aegypti
(0.0022
mg/L
0.004
mg/L),
quinquefasciatus
(0.0025
0.0044
mg/L).
SDS-PAGE
LC-MS
analysis
revealed
that
Cry11Aa5
(Pesticidal
Crystal
Protein)
predominant
toxin
this
strain.
PCR
amplification
confirmed
presence
genes
encoding
various
proteins,
including
Cry
Cyt
toxins.
Phylogenetic
performed
assess
genetic
relatedness
profiles
bacterial
isolate.
detailed
Bti
highlights
its
potential
as
a
promising
biopesticide
candidate
control,
contributing
development
sustainable
eco-friendly
strategies
vector
management.
Язык: Английский
Whole genome sequencing and evolutionary significance of a novel mosquitocidal bacterium, Bacillus thuringiensis serovar israelensis VCRC-B650 reported from Union Territory of Puducherry, India highly useful for mosquito control
Kakhuangailiu Gangmei,
Mathivanan Ashokkumar,
Bhavna Gupta
и другие.
Molecular Genetics and Genomics,
Год журнала:
2025,
Номер
300(1)
Опубликована: Март 26, 2025
Язык: Английский