Characterization of pesticidal crystal toxin protein Cry11Aa from Bacillus thuringiensis serovar israelensis VCRC-B646 for mosquito control.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Effective mosquito control is essential for reducing the transmission of vector-borne diseases. This study focuses on comprehensive characterization mosquitocidal toxins produced by Bacillus thuringiensis serovar israelensis (Bti) VCRC B646 and associated insecticidal genes. The bacterium was cultured, spore-crystal complex purified to identify proteins. isolate were effective against Aedes aegypti, Anopheles stephensi, Culex quinquefasciatus, Toxicity bioassays indicated lethal concentrations (LC₅₀ LC₉₀) aegypti (0.0022 mg/L 0.004 mg/L), quinquefasciatus (0.0025 0.0044 mg/L). SDS-PAGE LC-MS analysis revealed that Cry11Aa5 (Pesticidal Crystal Protein) predominant toxin this strain. PCR amplification confirmed presence genes encoding various proteins, including Cry Cyt toxins. Phylogenetic performed assess genetic relatedness profiles bacterial isolate. detailed Bti highlights its potential as a promising biopesticide candidate control, contributing development sustainable eco-friendly strategies vector management.

Language: Английский

Whole genome sequencing and evolutionary significance of a novel mosquitocidal bacterium, Bacillus thuringiensis serovar israelensis VCRC-B650 reported from Union Territory of Puducherry, India highly useful for mosquito control

Molecular Genetics and Genomics, Journal Year: 2025, Volume and Issue: 300(1)

Published: March 26, 2025

Language: Английский