Asian Journal of Surgery, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Molecular Carcinogenesis, Год журнала: 2025, Номер unknown
Опубликована: Янв. 21, 2025
ABSTRACT Colorectal cancer (CRC) is one of the most common malignancies. Hypoxia can promote occurrence and development CRC. However, how hypoxia regulates CRC immune microenvironment needs to be further explored. The bulk RNA sequencing data clinicopathological information patients were enrolled from Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases. single‐cell (scRNA‐seq) datasets collected analyzed GEO database ArrayExpress database. score gene set was estimated using “ssGSEA” algorithm in “GSVA” R package. functional characteristics CAF subtypes studied by bioinformatics analysis vitro experiments, a prognostic model constructed based on machine learning correlation. associated with poor prognosis patients. Periostin (POSTN) + Fib cancer‐associated fibroblast (CAF) closely hypoxia, high infiltration POSTN adverse outcomes overall survival (OS) relapse‐free (RFS) induce expression secretion CAFs. Hypoxia‐induced increase CAFs significantly migration proliferation cells. Hypoxia‐Related Risk Model (PFHRM) predict immunotherapy response Our study identified cell subpopulation which promotes malignant progression PFHRM provides theoretical basis for improving patient prognosis.
Язык: Английский
Процитировано
2
Cancers, Год журнала: 2024, Номер 16(18), С. 3205 - 3205
Опубликована: Сен. 20, 2024
Background: Colorectal cancer is one of the most prevalent forms and associated with a high mortality rate. Additionally, an increasing number adults under 50 are being diagnosed disease. This underscores importance leveraging modern technologies, such as artificial intelligence, for early diagnosis treatment support. Methods: Eight classifiers were utilized in this research: Random Forest, XGBoost, CatBoost, LightGBM, Gradient Boosting, Extra Trees, k-nearest neighbor algorithm (KNN), decision trees. These algorithms optimized using frameworks Optuna, RayTune, HyperOpt. study was conducted on public dataset from Brazil, containing information tens thousands patients. Results: The models developed demonstrated classification accuracy predicting one-, three-, five-year survival, well overall cancer-specific mortality. Forest delivered best performance, achieving approximately 80% across all evaluated tasks. Conclusions: research enabled development effective that can be applied clinical practice.
Язык: Английский
Процитировано
10
Biomedicines, Год журнала: 2025, Номер 13(1), С. 219 - 219
Опубликована: Янв. 16, 2025
Background/Objectives: Colorectal cancer (CRC) is one of the most common oncological disorders. Its fundamental treatments include surgery and chemotherapy, predominantly utilizing 5-fluorouracil (5-FU). Despite medical advances, CRC continues to present a high risk recurrence, metastasis low survival rates. Consequently, significant emphasis has been directed towards exploring novel types cell death, particularly ferroptosis. Ferroptosis characterized by iron imbalance accumulation lipid peroxides reactive oxygen species (ROS), leading cellular damage death. Thus, discovery safe inducers ferroptosis, offering new hope in struggle against CRC, remains crucial. In this study, we applied concept drug repositioning, selecting mesalazine (MES), non-steroidal anti-inflammatory (NSAID), for investigation. Methods: The study was conducted on colon line DLD-1 normal intestinal epithelial cells from CCD 841 CoN line. Both lines were treated with MES solutions at concentrations 10, 20, 30, 40, 50 mM. Cytotoxicity assessed using MTT assay, while ferroptosis-related gene expression analysis performed oligonucleotide microarrays, RT-qPCR used validation. Results: effectively reduces viability minimally affecting cells. Subsequent microarray revealed that significantly alters 56 genes associated Conclusions: Our results suggest may induce ferroptosis providing foundation further research area.
Язык: Английский
Процитировано
2
Biomedicine & Pharmacotherapy, Год журнала: 2025, Номер 186, С. 118014 - 118014
Опубликована: Март 31, 2025
Язык: Английский
Процитировано
1
International Immunopharmacology, Год журнала: 2024, Номер 143, С. 113325 - 113325
Опубликована: Окт. 14, 2024
Язык: Английский
Процитировано
8
Aggregate, Год журнала: 2025, Номер unknown
Опубликована: Янв. 15, 2025
ABSTRACT Colorectal cancer (CRC) screening and early diagnosis is an effective strategy for reducing CRC mortality. However, the current detection methods involve exorbitant costs complex procedures, which are inconvenient large‐scale screening. Given its high prevalence in malignant tissues feces of patients, Fusobacterium nucleatum ( F. ) has emerged as a crucial biomarker CRC. Herein, we propose ‐specific recognition diagnosis. A novel nanobioprobe (AIE‐Pep) with aggregation‐induced emission (AIE) characteristics was synthesized by conjugating red/near‐infrared (NIR) emissive AIE luminogen (AIEgen) FadA‐targeting peptide (ASANWTIQYND). The robust binding affinity between FadA on allows AIE‐Pep NPs to adhere selectively , emits strong red/NIR fluorescence. In model orthotopic CRC, can precisely localize around Moreover, demonstrated limit (LOD) 82.97 CFU/mL could significantly differentiate mice from those normal mice. Overall, this study presents pivotal approach specifically identifying holds immense potential
Язык: Английский
Процитировано
1
Frontiers in Oncology, Год журнала: 2024, Номер 14
Опубликована: Дек. 3, 2024
Over the past century, colorectal cancer (CRC) has become one of most devastating cancers impacting human population. To gain a deeper understanding molecular mechanisms driving this solid tumor, researchers have increasingly turned their attention to tumor microenvironment (TME). Spatial transcriptomics and proteomics emerged as particularly powerful technology for deciphering complexity CRC tumors, given that TME its spatial organization are critical determinants disease progression treatment response. enables high-resolution mapping whole transcriptome. While maps protein expression function across tissue sections. Together, they provide detailed view landscape cellular interactions within TME. In review, we delve into recent advances in biology technologies applied research, highlighting both methodologies challenges associated with use, such substantial heterogeneity characteristic CRC. We also discuss limitations current approaches need novel computational tools manage interpret these complex datasets. conclude, emphasize importance further developing integrating precision medicine strategies enhance therapeutic targeting improve patient outcomes.
Язык: Английский
Процитировано
6
Journal of Biochemical and Molecular Toxicology, Год журнала: 2025, Номер 39(1)
Опубликована: Янв. 1, 2025
Wilms tumor 1-associated protein (WTAP) has been validated to be a crucial regulator in the tumorigenesis and advancement of diverse malignancies. This study intended probe impacts WTAP on colorectal cancer (CRC) progression from perspective N6-methyladenosine (m6A) modification. The differential expression patterns clinical CRC samples cultured cell lines were via qRT-PCR western blot. Cell function tests conducted with colony formation, transwell, CCK-8. MeRIP-qPCR was identify WTAP-mediated SOD2 (Superoxide dismutase 2) mRNA modification cells. Animal experiments adopted evaluate vivo. exhibited high pattern along Silencing potently restrained growth virto Mechanically, identified as an m6A target WTAP. elevated its stability IGF2BP3-dependent manner. Meanwhile, overexpression could reverse suppressive effect induced by silencing. Molecular therapy targeting WTAP-SOD2 may offer novel insights perspectives for treatment CRC.
Язык: Английский
Процитировано
0
Bioorganic & Medicinal Chemistry, Год журнала: 2025, Номер 125, С. 118203 - 118203
Опубликована: Апрель 18, 2025
Язык: Английский
Процитировано
0
Journal of Functional Foods, Год журнала: 2025, Номер 129, С. 106857 - 106857
Опубликована: Май 15, 2025
Язык: Английский
Процитировано
0