Cellular Signalling, Journal Year: 2025, Volume and Issue: 132, P. 111817 - 111817
Published: April 16, 2025
Language: Английский
Biomedicines, Journal Year: 2025, Volume and Issue: 13(1), P. 219 - 219
Published: Jan. 16, 2025
Background/Objectives: Colorectal cancer (CRC) is one of the most common oncological disorders. Its fundamental treatments include surgery and chemotherapy, predominantly utilizing 5-fluorouracil (5-FU). Despite medical advances, CRC continues to present a high risk recurrence, metastasis low survival rates. Consequently, significant emphasis has been directed towards exploring novel types cell death, particularly ferroptosis. Ferroptosis characterized by iron imbalance accumulation lipid peroxides reactive oxygen species (ROS), leading cellular damage death. Thus, discovery safe inducers ferroptosis, offering new hope in struggle against CRC, remains crucial. In this study, we applied concept drug repositioning, selecting mesalazine (MES), non-steroidal anti-inflammatory (NSAID), for investigation. Methods: The study was conducted on colon line DLD-1 normal intestinal epithelial cells from CCD 841 CoN line. Both lines were treated with MES solutions at concentrations 10, 20, 30, 40, 50 mM. Cytotoxicity assessed using MTT assay, while ferroptosis-related gene expression analysis performed oligonucleotide microarrays, RT-qPCR used validation. Results: effectively reduces viability minimally affecting cells. Subsequent microarray revealed that significantly alters 56 genes associated Conclusions: Our results suggest may induce ferroptosis providing foundation further research area.
Language: Английский
Citations
2
Cancers, Journal Year: 2024, Volume and Issue: 16(18), P. 3205 - 3205
Published: Sept. 20, 2024
Background: Colorectal cancer is one of the most prevalent forms and associated with a high mortality rate. Additionally, an increasing number adults under 50 are being diagnosed disease. This underscores importance leveraging modern technologies, such as artificial intelligence, for early diagnosis treatment support. Methods: Eight classifiers were utilized in this research: Random Forest, XGBoost, CatBoost, LightGBM, Gradient Boosting, Extra Trees, k-nearest neighbor algorithm (KNN), decision trees. These algorithms optimized using frameworks Optuna, RayTune, HyperOpt. study was conducted on public dataset from Brazil, containing information tens thousands patients. Results: The models developed demonstrated classification accuracy predicting one-, three-, five-year survival, well overall cancer-specific mortality. Forest delivered best performance, achieving approximately 80% across all evaluated tasks. Conclusions: research enabled development effective that can be applied clinical practice.
Language: Английский
Citations
9
Aggregate, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 15, 2025
ABSTRACT Colorectal cancer (CRC) screening and early diagnosis is an effective strategy for reducing CRC mortality. However, the current detection methods involve exorbitant costs complex procedures, which are inconvenient large‐scale screening. Given its high prevalence in malignant tissues feces of patients, Fusobacterium nucleatum ( F. ) has emerged as a crucial biomarker CRC. Herein, we propose ‐specific recognition diagnosis. A novel nanobioprobe (AIE‐Pep) with aggregation‐induced emission (AIE) characteristics was synthesized by conjugating red/near‐infrared (NIR) emissive AIE luminogen (AIEgen) FadA‐targeting peptide (ASANWTIQYND). The robust binding affinity between FadA on allows AIE‐Pep NPs to adhere selectively , emits strong red/NIR fluorescence. In model orthotopic CRC, can precisely localize around Moreover, demonstrated limit (LOD) 82.97 CFU/mL could significantly differentiate mice from those normal mice. Overall, this study presents pivotal approach specifically identifying holds immense potential
Language: Английский
Citations
1
Molecular Carcinogenesis, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 21, 2025
ABSTRACT Colorectal cancer (CRC) is one of the most common malignancies. Hypoxia can promote occurrence and development CRC. However, how hypoxia regulates CRC immune microenvironment needs to be further explored. The bulk RNA sequencing data clinicopathological information patients were enrolled from Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases. single‐cell (scRNA‐seq) datasets collected analyzed GEO database ArrayExpress database. score gene set was estimated using “ssGSEA” algorithm in “GSVA” R package. functional characteristics CAF subtypes studied by bioinformatics analysis vitro experiments, a prognostic model constructed based on machine learning correlation. associated with poor prognosis patients. Periostin (POSTN) + Fib cancer‐associated fibroblast (CAF) closely hypoxia, high infiltration POSTN adverse outcomes overall survival (OS) relapse‐free (RFS) induce expression secretion CAFs. Hypoxia‐induced increase CAFs significantly migration proliferation cells. Hypoxia‐Related Risk Model (PFHRM) predict immunotherapy response Our study identified cell subpopulation which promotes malignant progression PFHRM provides theoretical basis for improving patient prognosis.
Language: Английский
Citations
1
International Immunopharmacology, Journal Year: 2024, Volume and Issue: 143, P. 113325 - 113325
Published: Oct. 14, 2024
Language: Английский
Citations
7
Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14
Published: Dec. 3, 2024
Over the past century, colorectal cancer (CRC) has become one of most devastating cancers impacting human population. To gain a deeper understanding molecular mechanisms driving this solid tumor, researchers have increasingly turned their attention to tumor microenvironment (TME). Spatial transcriptomics and proteomics emerged as particularly powerful technology for deciphering complexity CRC tumors, given that TME its spatial organization are critical determinants disease progression treatment response. enables high-resolution mapping whole transcriptome. While maps protein expression function across tissue sections. Together, they provide detailed view landscape cellular interactions within TME. In review, we delve into recent advances in biology technologies applied research, highlighting both methodologies challenges associated with use, such substantial heterogeneity characteristic CRC. We also discuss limitations current approaches need novel computational tools manage interpret these complex datasets. conclude, emphasize importance further developing integrating precision medicine strategies enhance therapeutic targeting improve patient outcomes.
Language: Английский
Citations
6
Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(1)
Published: Jan. 1, 2025
Wilms tumor 1-associated protein (WTAP) has been validated to be a crucial regulator in the tumorigenesis and advancement of diverse malignancies. This study intended probe impacts WTAP on colorectal cancer (CRC) progression from perspective N6-methyladenosine (m6A) modification. The differential expression patterns clinical CRC samples cultured cell lines were via qRT-PCR western blot. Cell function tests conducted with colony formation, transwell, CCK-8. MeRIP-qPCR was identify WTAP-mediated SOD2 (Superoxide dismutase 2) mRNA modification cells. Animal experiments adopted evaluate vivo. exhibited high pattern along Silencing potently restrained growth virto Mechanically, identified as an m6A target WTAP. elevated its stability IGF2BP3-dependent manner. Meanwhile, overexpression could reverse suppressive effect induced by silencing. Molecular therapy targeting WTAP-SOD2 may offer novel insights perspectives for treatment CRC.
Language: Английский
Citations
0
BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)
Published: Jan. 13, 2025
Colorectal cancer (CRC) is a prevalent malignancy worldwide, associated with significant morbidity and mortality. Cyclin-dependent kinase 1 (CDK1) plays crucial role in cell cycle regulation has been implicated various cancers. This study aimed to evaluate the prognostic value of CDK1 CRC identify traditional Chinese medicines (TCM) that can target as potential treatments for CRC. The expression were analyzed through TCGA, GEO, GEPIA, UALCAN HPA databases. An ESTIMATE analysis was applied estimate proportions stromal immune cells tumor samples. GO KEGG enrichment analyses performed clarify functional roles CDK1-related genes. CCK-8, colony formation, migration, invasion, wound healing assays employed explore tumor-promoting CDK1. Molecular docking, cellular thermal shift, isothermal dose-response inhibitors highly expressed poorer prognosis. also correlated levels infiltration. genes primarily involved P53 signaling pathway. Knockdown inhibited proliferation, invasion vitro. Furthermore, Eriocitrin emerged inhibitor, exerting its anti-tumor effects by targeting inhibiting activity. critical Eriocitrin, inhibitor derived from TCM, highlights promising new therapeutic strategy treatment.
Language: Английский
Citations
0
Pathology - Research and Practice, Journal Year: 2025, Volume and Issue: 268, P. 155870 - 155870
Published: Feb. 26, 2025
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2216 - 2216
Published: Feb. 28, 2025
Colorectal cancer (CRC) remains a leading cause of cancer-related mortality, with metastatic disease posing significant therapeutic challenges. While anti-EGFR therapy has improved outcomes for patients RAS and BRAF wild-type tumors, resistance major hurdle, limiting treatment efficacy. The concept negative hyperselection emerged as refinement molecular profiling, identifying additional genomic alterations-such HER2 MET amplificationsand MAP2K1 mutations-that predict to agents. Studies incorporating these expanded assessments have demonstrated that nearly half RAS/BRAF tumors harbor alternative biomarkers, underscoring the need selection criteria. Liquid biopsies, particularly circulating tumor DNA (ctDNA) analysis, revolutionized precision oncology by providing minimally invasive, real-time assessment dynamics. ctDNA-based enables detection resistance-associated alterations, guiding decisions greater accuracy than conventional tissue biopsies. Recent trials support predictive value ctDNA-defined hyperselection, revealing superior stratified through liquid biopsy. This narrative review explores evolving role in first-line therapy, emphasizing integration ctDNA refine patient selection, enhance efficacy, pave way personalized strategies CRC.
Language: Английский
Citations
0