Leukocyte Platelet‐Rich Plasma‐Derived Exosomes Restrained Macrophages Viability and Induced Apoptosis, NO Generation, and M1 Polarization DOI Creative Commons
Xiong Li, Feifei Guo,

Jiehua Deng

и другие.

Immunity Inflammation and Disease, Год журнала: 2024, Номер 12(11)

Опубликована: Ноя. 1, 2024

ABSTRACT Background Chronic refractory wounds refer to that cannot be repaired timely. Platelet‐rich plasma (PRP) has significant potential in chronic wound healing therapy. The exosomes isolated from PRP were proved exhibit more effectiveness than PRP. However, the therapeutic of on remained elusive. Hence, this study aimed clarify action by evaluating response macrophages exosomes. Methods Pure platelet‐rich (P‐PRP) and leukocyte (L‐PRP) prepared fasting venous blood healthy volunteers. Exosomes extracted P‐PRP L‐PRP using ultracentrifugation identified transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), western blot. Macrophages obtained inducing THP‐1 cells with phorbol‐12‐myristate‐13 acetate (PMA). internalization into was observed utilizing confocal laser scanning after being labeled PKH67. Cell viability determined CCK‐8 assay. apoptosis measured a flow cytometer. polarization status M1 M2 evaluated detecting their markers. Nitric oxide (NO) detection conducted commercial kit. Results absorbed macrophages. restrained induced Besides, promoted polarization, polarization. Furthermore, NO generation Conclusion viability, macrophages, while increased presented effective effect P‐PRP, making it promising strategy for management.

Язык: Английский

Genetics, sex and the use of platelet‐rich plasma influence the development of arthrofibrosis after anterior cruciate ligament reconstruction DOI Creative Commons
Mikel Sánchez,

Izarbe Yarza,

Cristina Jorquera

и другие.

Journal of Experimental Orthopaedics, Год журнала: 2025, Номер 12(1)

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Leukocyte Platelet‐Rich Plasma‐Derived Exosomes Restrained Macrophages Viability and Induced Apoptosis, NO Generation, and M1 Polarization DOI Creative Commons
Xiong Li, Feifei Guo,

Jiehua Deng

и другие.

Immunity Inflammation and Disease, Год журнала: 2024, Номер 12(11)

Опубликована: Ноя. 1, 2024

ABSTRACT Background Chronic refractory wounds refer to that cannot be repaired timely. Platelet‐rich plasma (PRP) has significant potential in chronic wound healing therapy. The exosomes isolated from PRP were proved exhibit more effectiveness than PRP. However, the therapeutic of on remained elusive. Hence, this study aimed clarify action by evaluating response macrophages exosomes. Methods Pure platelet‐rich (P‐PRP) and leukocyte (L‐PRP) prepared fasting venous blood healthy volunteers. Exosomes extracted P‐PRP L‐PRP using ultracentrifugation identified transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), western blot. Macrophages obtained inducing THP‐1 cells with phorbol‐12‐myristate‐13 acetate (PMA). internalization into was observed utilizing confocal laser scanning after being labeled PKH67. Cell viability determined CCK‐8 assay. apoptosis measured a flow cytometer. polarization status M1 M2 evaluated detecting their markers. Nitric oxide (NO) detection conducted commercial kit. Results absorbed macrophages. restrained induced Besides, promoted polarization, polarization. Furthermore, NO generation Conclusion viability, macrophages, while increased presented effective effect P‐PRP, making it promising strategy for management.

Язык: Английский

Процитировано

1