Leukocyte Platelet‐Rich Plasma‐Derived Exosomes Restrained Macrophages Viability and Induced Apoptosis, NO Generation, and M1 Polarization DOI Creative Commons
Xiong Li, Feifei Guo,

Jiehua Deng

et al.

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(11)

Published: Nov. 1, 2024

ABSTRACT Background Chronic refractory wounds refer to that cannot be repaired timely. Platelet‐rich plasma (PRP) has significant potential in chronic wound healing therapy. The exosomes isolated from PRP were proved exhibit more effectiveness than PRP. However, the therapeutic of on remained elusive. Hence, this study aimed clarify action by evaluating response macrophages exosomes. Methods Pure platelet‐rich (P‐PRP) and leukocyte (L‐PRP) prepared fasting venous blood healthy volunteers. Exosomes extracted P‐PRP L‐PRP using ultracentrifugation identified transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), western blot. Macrophages obtained inducing THP‐1 cells with phorbol‐12‐myristate‐13 acetate (PMA). internalization into was observed utilizing confocal laser scanning after being labeled PKH67. Cell viability determined CCK‐8 assay. apoptosis measured a flow cytometer. polarization status M1 M2 evaluated detecting their markers. Nitric oxide (NO) detection conducted commercial kit. Results absorbed macrophages. restrained induced Besides, promoted polarization, polarization. Furthermore, NO generation Conclusion viability, macrophages, while increased presented effective effect P‐PRP, making it promising strategy for management.

Language: Английский

Genetics, sex and the use of platelet‐rich plasma influence the development of arthrofibrosis after anterior cruciate ligament reconstruction DOI Creative Commons
Mikel Sánchez,

Izarbe Yarza,

Cristina Jorquera

et al.

Journal of Experimental Orthopaedics, Journal Year: 2025, Volume and Issue: 12(1)

Published: Jan. 1, 2025

Language: Английский

Citations

0
Leukocyte Platelet‐Rich Plasma‐Derived Exosomes Restrained Macrophages Viability and Induced Apoptosis, NO Generation, and M1 Polarization DOI Creative Commons
Xiong Li, Feifei Guo,

Jiehua Deng

et al.

Immunity Inflammation and Disease, Journal Year: 2024, Volume and Issue: 12(11)

Published: Nov. 1, 2024

ABSTRACT Background Chronic refractory wounds refer to that cannot be repaired timely. Platelet‐rich plasma (PRP) has significant potential in chronic wound healing therapy. The exosomes isolated from PRP were proved exhibit more effectiveness than PRP. However, the therapeutic of on remained elusive. Hence, this study aimed clarify action by evaluating response macrophages exosomes. Methods Pure platelet‐rich (P‐PRP) and leukocyte (L‐PRP) prepared fasting venous blood healthy volunteers. Exosomes extracted P‐PRP L‐PRP using ultracentrifugation identified transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), western blot. Macrophages obtained inducing THP‐1 cells with phorbol‐12‐myristate‐13 acetate (PMA). internalization into was observed utilizing confocal laser scanning after being labeled PKH67. Cell viability determined CCK‐8 assay. apoptosis measured a flow cytometer. polarization status M1 M2 evaluated detecting their markers. Nitric oxide (NO) detection conducted commercial kit. Results absorbed macrophages. restrained induced Besides, promoted polarization, polarization. Furthermore, NO generation Conclusion viability, macrophages, while increased presented effective effect P‐PRP, making it promising strategy for management.

Language: Английский

Citations

1