Journal of Cellular Biotechnology,
Год журнала:
2023,
Номер
10(1), С. 45 - 74
Опубликована: Дек. 1, 2023
BACKGROUND:
Biosurfactants
are
surfactants
derived
from
several
types
of
microorganisms
such
as
bacteria,
yeasts
and
fungi
membrane
components
or
secondary
metabolites.
OBJECTIVE:
To
increase
BS
productivity
a
biocompatible,
low-toxic
substitute
for
chemical
employed
in
modern
industry
the
huge
variety
applications
they
used
in.
METHODS:
Different
media
were
isolation
biosurfactant
producers.
The
most
potent
bacterial
isolate
was
analysed
by
16S-rRNA.
Plackett
Burman
Box-Behnken
Designs
optimization
conditions.
purified
characterized.
RESULTS:
Only
one
strain
demonstrated
great
productivity,
excellent
emulsifying
capability
(54.5±0.1%)
oil
spreading
activity
which
Bacillus
cereus
AHMNAZ1
with
accession
number
OP714421.1.
lipopeptide
nature,
identified
surfactin
stable
has
Critical
Micelle
Concentration
60
mg/L.
Isolated
showed
against
Colon
Carcinoma
Breast
cell
lines
can
be
antimicrobial
agent.
CONCLUSION:
study
found
cheaper
way
creating
biosurfactants
agro-industrial
wastes,
delivering
twofold
benefit
lowering
environmental
pollution
manufacturing
useful
biotechnological
products
(biosurfactants)
high
thermostability.
Moreover,
it
bioremediation
oil-polluted
soils
medical
fields.
Water Practice & Technology,
Год журнала:
2024,
Номер
19(3), С. 629 - 645
Опубликована: Фев. 24, 2024
Abstract
The
purification
and
characterization
of
lipopeptide
biosurfactants
produced
by
Bacillus
sp.
MI27
permits
to
identify
two
clusters
cyclic
linear
surfactin
isoforms.
Aiming
for
a
potential
application
in
industrial
biotechnology,
we
studied
its
washing
capacity.
results
show
good
ability
wash
oil
stains,
motor
mayonnaise
evaluated
the
determination
%
stain
removal
tissue
bleaching
indices
after
washing.
Optimal
capacities
were
unregistered
300
400
mg/L
at
pH
values
ranging
from
5
9
temperatures
20
40°C.
Using
4-factor
mixing
design,
optimized
liquid
formula
composed
only
BioS;
silicate;
carboxymethyl
cellulose
calcium
phosphate.
efficiency
is
order
43.46%
petroleum
49.10%
with
percentage
reduction
surface
tension
about
55.79%
corresponding
25.7
mN/m.
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 12, 2023
Abstract
Microbial
surfactants
are
natural
amphiphilic
compounds
with
high
surface
activities
and
emulsifying
properties.
Due
to
their
structural
diversity,
low
toxicity,
biodegradability,
chemical
stability
in
different
conditions,
these
molecules
potential
substitutes
for
surfactants;
interest
has
grown
significantly
over
the
last
decade.
The
current
study
focuses
on
isolation,
identification,
characterization
of
a
lactic
acid
bacteria
that
produces
two
forms
biosurfactants.
OL5
strain
was
isolated
from
green
olive
fermentation
identified
using
MALDI/TOF
ADNr16S
amplification.
Emulsification
activity
tension
measurements
were
used
estimate
biosurfactant
production.
biosurfactants
derived
L.
plantarum
presented
good
emulsification
powers
presence
various
oils.
They
also
shown
have
reduce
water
69
mN/m
34
37
within
critical
micelle
concentration
(CMC)
7
mg/ml
1.8
mg/ml,
respectively,
cell
bound
extracellular
Thin
layer
chromatography
(TLC)
FT-IR
analyze
composition
produced.
obtained
data
revealed
biomolecules
consist
mixture
carbohydrates,
lipids
proteins.
We
demonstrated
they
anionic
glycolipopeptide
nature
which
stable
extreme
conditions
temperature,
pH
salinity.
Journal of Cellular Biotechnology,
Год журнала:
2023,
Номер
10(1), С. 45 - 74
Опубликована: Дек. 1, 2023
BACKGROUND:
Biosurfactants
are
surfactants
derived
from
several
types
of
microorganisms
such
as
bacteria,
yeasts
and
fungi
membrane
components
or
secondary
metabolites.
OBJECTIVE:
To
increase
BS
productivity
a
biocompatible,
low-toxic
substitute
for
chemical
employed
in
modern
industry
the
huge
variety
applications
they
used
in.
METHODS:
Different
media
were
isolation
biosurfactant
producers.
The
most
potent
bacterial
isolate
was
analysed
by
16S-rRNA.
Plackett
Burman
Box-Behnken
Designs
optimization
conditions.
purified
characterized.
RESULTS:
Only
one
strain
demonstrated
great
productivity,
excellent
emulsifying
capability
(54.5±0.1%)
oil
spreading
activity
which
Bacillus
cereus
AHMNAZ1
with
accession
number
OP714421.1.
lipopeptide
nature,
identified
surfactin
stable
has
Critical
Micelle
Concentration
60
mg/L.
Isolated
showed
against
Colon
Carcinoma
Breast
cell
lines
can
be
antimicrobial
agent.
CONCLUSION:
study
found
cheaper
way
creating
biosurfactants
agro-industrial
wastes,
delivering
twofold
benefit
lowering
environmental
pollution
manufacturing
useful
biotechnological
products
(biosurfactants)
high
thermostability.
Moreover,
it
bioremediation
oil-polluted
soils
medical
fields.
