Isolation and characterization of two glycolipopeptids biosurfactants produced by a Lactobacillus plantarum strain isolated from green olive curing water

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: April 12, 2023

Abstract Microbial surfactants are natural amphiphilic compounds with high surface activities and emulsifying properties. Due to their structural diversity, low toxicity, biodegradability, chemical stability in different conditions, these molecules potential substitutes for surfactants; interest has grown significantly over the last decade. The current study focuses on isolation, identification, characterization of a lactic acid bacteria that produces two forms biosurfactants. OL5 strain was isolated from green olive fermentation identified using MALDI/TOF ADNr16S amplification. Emulsification activity tension measurements were used estimate biosurfactant production. biosurfactants derived L. plantarum presented good emulsification powers presence various oils. They also shown have reduce water 69 mN/m 34 37 within critical micelle concentration (CMC) 7 mg/ml 1.8 mg/ml, respectively, cell bound extracellular Thin layer chromatography (TLC) FT-IR analyze composition produced. obtained data revealed biomolecules consist mixture carbohydrates, lipids proteins. We demonstrated they anionic glycolipopeptide nature which stable extreme conditions temperature, pH salinity.

Language: Английский

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Eco-friendly treatment of synthetic dyes contaminated water by biosorption: use of Bacillus mojavensis BI2 derived lipopeptide and date palm waste flour as biosorbents

Journal of Dispersion Science and Technology, Journal Year: 2023, Volume and Issue: 45(6), P. 1214 - 1226

Published: May 7, 2023

In the former section of present paper, prospective application B. mojavensis BI2 derived lipopeptides for dyes biodecolorization was demonstrated. The lipopeptide mainly composed linear and cyclic Fengycin Surfactin isoforms. When using 100 mg/L, we are able to remove about 98% 200 mg/L Malachite Green (MG). For Crystal Violet (CV), % does not exceed 85% in presence 400 lipopeptide. second part proved efficiency biosorption by palm waste floor, optimized a Taguichi design. bio-treatment evaluated color removal enhancement germination index. Optimum treatments were obtained with 10 g/L 6 MG CV respectively during 1 hour. Nevertheless, addition has no significant effect on date flour.

Language: Английский

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Surfactin production from Bacillus cereus AHMNAZ1 and its potential applications

Journal of Cellular Biotechnology, Journal Year: 2023, Volume and Issue: 10(1), P. 45 - 74

Published: Dec. 1, 2023

BACKGROUND: Biosurfactants are surfactants derived from several types of microorganisms such as bacteria, yeasts and fungi membrane components or secondary metabolites. OBJECTIVE: To increase BS productivity a biocompatible, low-toxic substitute for chemical employed in modern industry the huge variety applications they used in. METHODS: Different media were isolation biosurfactant producers. The most potent bacterial isolate was analysed by 16S-rRNA. Plackett Burman Box-Behnken Designs optimization conditions. purified characterized. RESULTS: Only one strain demonstrated great productivity, excellent emulsifying capability (54.5±0.1%) oil spreading activity which Bacillus cereus AHMNAZ1 with accession number OP714421.1. lipopeptide nature, identified surfactin stable has Critical Micelle Concentration 60 mg/L. Isolated showed against Colon Carcinoma Breast cell lines can be antimicrobial agent. CONCLUSION: study found cheaper way creating biosurfactants agro-industrial wastes, delivering twofold benefit lowering environmental pollution manufacturing useful biotechnological products (biosurfactants) high thermostability. Moreover, it bioremediation oil-polluted soils medical fields.

Language: Английский

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