Hemocompatibility studies in nanotoxicology: Hemolysis or eryptosis? (A review)

Toxicology in Vitro, Год журнала: 2024, Номер 98, С. 105814 - 105814

Опубликована: Апрель 4, 2024

Язык: Английский

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Cell death signaling in human erythron: erythrocytes lose the complexity of cell death machinery upon maturation

APOPTOSIS, Год журнала: 2025, Номер unknown

Опубликована: Фев. 9, 2025

Abstract Over the recent years, our understanding of cell death machinery mature erythrocytes has been greatly expanded. It resulted in discovery several regulated (RCD) pathways red blood cells. Apoptosis (eryptosis) and necroptosis share certain features with their counterparts nucleated cells, but they are also critically different particular details. In this review article, we summarize subroutines erythroid precursors (apoptosis, necroptosis, ferroptosis) comparison to (eryptosis erythronecroptosis) highlight consequences organelle clearance associated loss multiple components upon erythrocyte maturation. Recent advances role RCDs health disease have expanded potential clinical applications these lethal subroutines, emphasizing contribution development anemia, microthrombosis, endothelial dysfunction, as well diagnostic biomarkers markers storage-induced lesions. Fas signaling functional caspase-8/caspase-3 system not indispensable for eryptosis, might be retained mediate crosstalk between both erythrocyte-associated RCDs. The ability switch eryptosis suggests that is a simple unregulated mechanical disintegration, tightly controlled process. This allows investigation eventual pharmacological interventions aimed at individual erythrocytes.

Язык: Английский

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Pyroptosis for osteoarthritis treatment: insights into cellular and molecular interactions inflammatory

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 1, 2025

Osteoarthritis (OA) is a widely prevalent chronic degenerative disease often associated with significant pain and disability. It characterized by the deterioration of cartilage extracellular matrix (ECM), synovial inflammation, subchondral bone remodeling. Recent studies have highlighted pyroptosis-a form programmed cell death triggered inflammasome-as key factor in sustaining inflammation. Central to this process are inflammatory cytokines interleukin-1β (IL-1β) interleukin-18 (IL-18), which play crucial roles mediating intra-articular pyroptosis through NOD-like receptor protein 3 (NLRP3) inflammasome. This paper investigates role pathway perpetuating diseases its linkage OA. Furthermore, it explores mechanisms pyroptosis, mediated nuclear κB (NF-κB), purinergic P2X ligand-gated ion channel 7 (P2X7R), adenosine monophosphate (AMP)-activated kinase (AMPK), hypoxia-inducible factor-1α (HIF-1α). Additionally, examines interactions among various cellular components context These insights indicate that targeting regulation presents promising therapeutic approach for prevention treatment OA, offering valuable theoretical perspectives effective management.

Язык: Английский

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Mn3O4 Nanocrystal-Induced Eryptosis Features Ca2+ Overload, ROS and RNS Accumulation, Calpain Activation, Recruitment of Caspases, and Changes in the Lipid Order of Cell Membranes

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3284 - 3284

Опубликована: Апрель 1, 2025

Accumulating evidence suggests that manganese oxide nanoparticles (NPs) show multiple enzyme-mimicking antioxidant activities, which supports their potential in redox-targeting therapeutic strategies for diseases with impaired redox signaling. However, the systemic administration of any NP requires thorough hemocompatibility testing. In this study, we assessed synthesized Mn3O4 NPs, identifying ability to induce spontaneous hemolysis and eryptosis or impair osmotic fragility. Concentrations up 20 mg/L were found be safe erythrocytes. Eryptosis assays shown more sensitive than fragility as markers Flow cytometry- confocal microscopy-based studies revealed induced by NPs was accompanied Ca2+ overload, altered homeostasis verified enhanced intracellular reactive oxygen species (ROS) nitrogen (RNS), a decrease lipid order cell membranes. Furthermore, NP-induced calpain- caspase-dependent.

Язык: Английский

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Particulate matter 2.5 stimulates pyroptosis and necroptosis via the p38 MAPK/Akt/NF-κB signaling pathway in human corneal epithelial cells

Toxicology, Год журнала: 2025, Номер unknown, С. 154138 - 154138

Опубликована: Апрель 1, 2025

Язык: Английский

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Nanomaterials as Modulators of Non-apoptotic Regulated Cell Death Modes: Novel Options in Cancer Treatment

Interdisciplinary cancer research, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Arctigenin-induced erythrocyte membrane remodeling is mediated through calcium influx, metabolic collapse, and casein kinase 1α

Xenobiotica, Год журнала: 2025, Номер unknown, С. 1 - 11

Опубликована: Май 31, 2025

1. Conclusive evidence suggests that arctigenin (AGN) holds great promise in anticancer therapy but a common and poorly understood complication of chemotherapy is anemia which precipitated by eryptosis hemolysis. This study examines the cytotoxicity AGN RBCs.2. Eryptosis markers including intracellular calcium, phosphatidylserine (PS) externalization, cell shrinkage were detected flow cytometry using Fluo4/AM, annexin-V-FITC, forward light scatter, respectively. Membrane blebbing was examined electron microscopy, photometric potentiometric methods used to assay hemolytic hemoglobin, potassium, AST, LDH.3. significantly increased Fluo4- annexin-V-positive cells decreased scatter associated with membrane blebs. While PS externalization inhibited extracellular calcium exclusion, suppression hemolysis required both exclusion restriction potassium efflux. Moreover, sucrose mannitol rescued from while exacerbating rather blunted guanosine CK1α inhibitor D4476.4. promotes calcium-dependent through energy depletion activation, exhibits potent activity dysregulated ion trafficking osmotic stress. These findings underscore hematological toxicity identify potential inhibitors inform future animal studies clinical trials.

Язык: Английский

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An Overview of Hexavalent Chromium-Induced Necroptosis, Pyroptosis, and Ferroptosis

Biological Trace Element Research, Год журнала: 2024, Номер unknown

Опубликована: Сен. 17, 2024

Язык: Английский

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Perturbation of calcium homeostasis invokes eryptosis-like cell death in enucleated bone marrow stem cells

Biochemistry and Cell Biology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

Enucleated cells, also known as cytoplasts, are valuable tools with a wide range of applications. However, their potential for bio-engineering is greatly restricted by the short lifespan. We postulated that enucleation process damages integrity plasma membrane and thus activates cell death program(s). The results showed tiny hole was generated transiently on when nucleus spun off, while force-gated ion channels were activated in response to pulling nucleus. Influx extracellular calcium stimulated opening release from endoplasmic reticulum mitochondria. Long lasting transient increased protein phosphorylation caspase 9 calpain proteinase activities. Subsequently, mitochondria permeability Reactive Oxygen Species (ROS) levels significantly elevated, which eventually led eryptosis-like death. When maintained at optimal concentration, lifespan enucleated cells extended; however, huge amounts vacuoles appeared cytoplasm, possibly derived enlarged autophagosomes. Inhibition vacuolation inhibitors autophagy or co-culture primary muscle did not rescue dying paraptosis-like pathway. These offer insights further investigation into intricate mechanisms underlying

Язык: Английский

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Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июль 18, 2024

Introduction. Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity newly synthesized nanostructured carbonate hydroxide (ZnCH) Zn₅(CO₃)(OH)₆ microflakes. Materials and methods. Cytotoxicity microflakes was against murine L929 cell line rat mature erythrocytes. MTT neutral red uptake assays, scratch assay, Annexin V-FITC/7-aminoactinomycin D staining, 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) Fura 2-AM staining reactive nitrogen species (RNS), detection caspases, lipid order-sensitive NR12S probe were employed evaluate viability, motility, death pathways, implication Ca²⁺, oxygen (ROS) RNS signaling, alterations membranes following exposure microflakes, respectively. To assess hemocompatibility Zn-containing osmotic fragility assay hemolysis performed, well multiple eryptosis parameters evaluated. Results. Our findings indicate dose-response ZnCH cells with no toxicity observed low concentrations (10 mg/L below). At high concentrations, promoted oxidative stress, Ca²⁺- caspase-dependent apoptosis altered membranes. Surprisingly, enucleated erythrocytes found lower compared cells. induced associated Ca²⁺ signaling recruitment caspases. Eryptosis assays more sensitive than evaluation hemolysis. Conclusions. show at indicating their potential

Язык: Английский

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