The Effects of Pb on TNF-R1-RIPK1/RIPK3 Signaling Pathway in the Hippocampus of Mice DOI

Huishuai Li,

Zhenning Li, Chun Yang

et al.

Neurochemical Research, Journal Year: 2024, Volume and Issue: 50(1)

Published: Nov. 27, 2024

Language: Английский

Cell death signaling in human erythron: erythrocytes lose the complexity of cell death machinery upon maturation DOI Creative Commons
Anton Tkachenko, Ondřej Havránek

APOPTOSIS, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Abstract Over the recent years, our understanding of cell death machinery mature erythrocytes has been greatly expanded. It resulted in discovery several regulated (RCD) pathways red blood cells. Apoptosis (eryptosis) and necroptosis share certain features with their counterparts nucleated cells, but they are also critically different particular details. In this review article, we summarize subroutines erythroid precursors (apoptosis, necroptosis, ferroptosis) comparison to (eryptosis erythronecroptosis) highlight consequences organelle clearance associated loss multiple components upon erythrocyte maturation. Recent advances role RCDs health disease have expanded potential clinical applications these lethal subroutines, emphasizing contribution development anemia, microthrombosis, endothelial dysfunction, as well diagnostic biomarkers markers storage-induced lesions. Fas signaling functional caspase-8/caspase-3 system not indispensable for eryptosis, might be retained mediate crosstalk between both erythrocyte-associated RCDs. The ability switch eryptosis suggests that is a simple unregulated mechanical disintegration, tightly controlled process. This allows investigation eventual pharmacological interventions aimed at individual erythrocytes.

Language: Английский

Citations

1
Hemocompatibility studies in nanotoxicology: Hemolysis or eryptosis? (A review) DOI
Anton Tkachenko

Toxicology in Vitro, Journal Year: 2024, Volume and Issue: 98, P. 105814 - 105814

Published: April 4, 2024

Language: Английский

Citations

8
Particulate matter 2.5 stimulates pyroptosis and necroptosis via the p38 MAPK/Akt/NF-κB signaling pathway in human corneal epithelial cells DOI
Da Hye Kim, Hyesook Lee, Min Yeong Kim

et al.

Toxicology, Journal Year: 2025, Volume and Issue: unknown, P. 154138 - 154138

Published: April 1, 2025

Language: Английский

Citations

0
Nanomaterials as Modulators of Non-apoptotic Regulated Cell Death Modes: Novel Options in Cancer Treatment DOI
Anton Tkachenko

Interdisciplinary cancer research, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
An Overview of Hexavalent Chromium-Induced Necroptosis, Pyroptosis, and Ferroptosis DOI

Saulesh Kurmangaliyeva,

Kristina Baktikulova,

Viktoriya Tkachenko

et al.

Biological Trace Element Research, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 17, 2024

Language: Английский

Citations

3
Pyroptosis for osteoarthritis treatment: insights into cellular and molecular interactions inflammatory DOI Creative Commons
Minghui Lin, Cunxin Zhang, Haiming Li

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

Osteoarthritis (OA) is a widely prevalent chronic degenerative disease often associated with significant pain and disability. It characterized by the deterioration of cartilage extracellular matrix (ECM), synovial inflammation, subchondral bone remodeling. Recent studies have highlighted pyroptosis-a form programmed cell death triggered inflammasome-as key factor in sustaining inflammation. Central to this process are inflammatory cytokines interleukin-1β (IL-1β) interleukin-18 (IL-18), which play crucial roles mediating intra-articular pyroptosis through NOD-like receptor protein 3 (NLRP3) inflammasome. This paper investigates role pathway perpetuating diseases its linkage OA. Furthermore, it explores mechanisms pyroptosis, mediated nuclear κB (NF-κB), purinergic P2X ligand-gated ion channel 7 (P2X7R), adenosine monophosphate (AMP)-activated kinase (AMPK), hypoxia-inducible factor-1α (HIF-1α). Additionally, examines interactions among various cellular components context These insights indicate that targeting regulation presents promising therapeutic approach for prevention treatment OA, offering valuable theoretical perspectives effective management.

Language: Английский

Citations

0
Mn3O4 Nanocrystal-Induced Eryptosis Features Ca2+ Overload, ROS and RNS Accumulation, Calpain Activation, Recruitment of Caspases, and Changes in the Lipid Order of Cell Membranes DOI Open Access

Yuriy Kot,

Volodymyr Prokopіuk, Vladimir Klochkov

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3284 - 3284

Published: April 1, 2025

Accumulating evidence suggests that manganese oxide nanoparticles (NPs) show multiple enzyme-mimicking antioxidant activities, which supports their potential in redox-targeting therapeutic strategies for diseases with impaired redox signaling. However, the systemic administration of any NP requires thorough hemocompatibility testing. In this study, we assessed synthesized Mn3O4 NPs, identifying ability to induce spontaneous hemolysis and eryptosis or impair osmotic fragility. Concentrations up 20 mg/L were found be safe erythrocytes. Eryptosis assays shown more sensitive than fragility as markers Flow cytometry- confocal microscopy-based studies revealed induced by NPs was accompanied Ca2+ overload, altered homeostasis verified enhanced intracellular reactive oxygen species (ROS) nitrogen (RNS), a decrease lipid order cell membranes. Furthermore, NP-induced calpain- caspase-dependent.

Language: Английский

Citations

0
Perturbation of calcium homeostasis invokes eryptosis-like cell death in enucleated bone marrow stem cells DOI
Wei Yan,

Ruolan Wu,

Ying‐Ying Lee

et al.

Biochemistry and Cell Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 18, 2024

Enucleated cells, also known as cytoplasts, are valuable tools with a wide range of applications. However, their potential for bio-engineering is greatly restricted by the short lifespan. We postulated that enucleation process damages integrity plasma membrane and thus activates cell death program(s). The results showed tiny hole was generated transiently on when nucleus spun off, while force-gated ion channels were activated in response to pulling nucleus. Influx extracellular calcium stimulated opening release from endoplasmic reticulum mitochondria. Long lasting transient increased protein phosphorylation caspase 9 calpain proteinase activities. Subsequently, mitochondria permeability Reactive Oxygen Species (ROS) levels significantly elevated, which eventually led eryptosis-like death. When maintained at optimal concentration, lifespan enucleated cells extended; however, huge amounts vacuoles appeared cytoplasm, possibly derived enlarged autophagosomes. Inhibition vacuolation inhibitors autophagy or co-culture primary muscle did not rescue dying paraptosis-like pathway. These offer insights further investigation into intricate mechanisms underlying

Language: Английский

Citations

2
The Role of TNF-R1-RIPK1/RIPK3 Signaling Pathway in Lead-Induced Necroptosis in the Hippocampus of Mice DOI Creative Commons

Huishuai Li,

Zhenning Li, Chun Yang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 19, 2024

Abstract Lead (Pb), a dense, soft, blue-gray metal, is widely used in metallurgy, cables, storage batteries, pigments, and other industrial applications. Pb has been shown to cause hippocampal neurodegeneration. Necroptosis, newly described cell death modality, closely associated with neurodegenerative diseases. Whether can programmed neuronal its mechanisms yet be characterized. Here, we explored the role of TNF-R1-RIPK1/RIPK3 signaling pathway necroptosis induced by Pb. Our results showed that exposure elevated lead levels murine whole blood tissue dose-response relationship. Protein expression PARP, c-PARP, RIPK1, p-RIPK1, RIPK3, MLKL, p-MLKL tissues were elevated, while protein caspase-8 was decreased. Furthermore, reduced survival rates HT-22 cells primary mouse neurons, increasing expressions RIPK1 p-MLKL. Collectively, these novel findings suggest TNF-R1/RIPK1/RIPK3 mediates Pb-induced neurons mice.

Language: Английский

Citations

0
Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro DOI
Volodymyr Prokopіuk, Anatolii Onishchenko,

Yuliia Pazura

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: July 18, 2024

Abstract Introduction. Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity newly synthesized nanostructured carbonate hydroxide (ZnCH) Zn5(CO3)(OH)6 microflakes. Materials and methods. Cytotoxicity microflakes was against murine L929 cell line rat mature erythrocytes. MTT neutral red uptake assays, scratch assay, Annexin V-FITC/7-aminoactinomycin D staining, 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) Fura 2-AM staining reactive nitrogen species (RNS), detection caspases, lipid order-sensitive NR12S probe were employed evaluate viability, motility, death pathways, implication Ca2+, oxygen (ROS) RNS signaling, alterations membranes following exposure microflakes, respectively. To assess hemocompatibility Zn-containing osmotic fragility assay hemolysis performed, well multiple eryptosis parameters evaluated. Results. Our findings indicate dose-response ZnCH cells with no toxicity observed low concentrations (10 mg/L below). At high concentrations, promoted oxidative stress, Ca2+- caspase-dependent apoptosis altered membranes. Surprisingly, enucleated erythrocytes found lower compared cells. induced associated Ca2+ signaling recruitment caspases. Eryptosis assays more sensitive than evaluation hemolysis. Conclusions. show at indicating their potential

Language: Английский

Citations

0