Genotyping of Candida tropicalis isolates uncovers nosocomial transmission of two lineages in Italian tertiary-care hospital

Journal of Hospital Infection, Год журнала: 2024, Номер 155, С. 115 - 122

Опубликована: Окт. 18, 2024

Candida tropicalis is a medically important yeast with increasing antifungal resistance, but nosocomial transmission rarely reported. This study genotyped C. isolates from Italian hospitals to uncover potential and assess resistance. In total, 197 161 patients were collected five centres 2013 2023. Short tandem repeat (STR) genotyping was conducted on all isolates, selection of 24 typed whole-genome sequencing (WGS) the novel Fourier-transform infrared (FTIR) spectroscopy method. Antifungal resistance investigated microbroth dilution WGS. STR revealed seven clusters multiple patients. WGS single nucleotide polymorphism (SNP) analysis groups related genotypes also separated these into groups, which two contained cluster different distinguished by ≤59 SNPs. comparison, sequential within three differentiated ≤141 The clustered based FTIR genotyping, although this method did not separate groups. None resistant common antifungals. SNP indicated lineages same hospital, highlighting need for enforced infection prevention measures routine in clinical settings. While both lineages, required determine whether transmitted clonally.

Язык: Английский

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Cluster of clonal fluconazole-resistant Candida parapsilosis harbouring Y132F mutation in ERG11 gene in a neonatal ICU in the UK

Journal of Antimicrobial Chemotherapy, Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Язык: Английский

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Evaluation of Genotyping Methods for Tracking the Spread of Sporothrix During Epidemics and Outbreaks

Fungal Biology, Год журнала: 2025, Номер unknown, С. 101566 - 101566

Опубликована: Март 1, 2025

Язык: Английский

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Genetic Epidemiology and Resistance Investigations of Clinical Yeasts in Alexandria, Egypt

Pathogens, Год журнала: 2025, Номер 14(5), С. 486 - 486

Опубликована: Май 15, 2025

Yeast bloodstream infections lead to high mortality and morbidity are mostly observed in immunocompromised patients. In Africa, only a few studies have characterized clinical yeasts. To increase insight into yeast resistance transmission we identified various yeasts from Alexandria, Egypt performed antifungal susceptibility testing (AFST) genotyping. A total of 1307 single isolates unique patients, recovered different anatomical sites including the bloodstream, retrieved reference laboratory were studied. All with MALDI-TOF MS, while some initially Vitek 2 Compact system. Short tandem repeat (STR) genotyping was for most common species, AFST microbroth dilution. Among (n = 71), C. albicans etiological agent, followed by tropicalis parapsilosis. Comparison identification methods demonstrated that 22% incorrectly system compared MS. Multiple rare showed reduced susceptibility. STR potential events nosocomial N. glabratus Moreover, an azole-resistant clade earlier Alexandria still present. conclude, Egypt, overall susceptible species.

Язык: Английский

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“CLADE-FINDER”: Candida auris Lineage Analysis Determination by Fourier Transform Infrared Spectroscopy and Artificial Neural Networks

Microorganisms, Год журнала: 2024, Номер 12(11), С. 2153 - 2153

Опубликована: Окт. 26, 2024

In 2019,

Язык: Английский

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Follow the Path: Unveiling an Azole Resistant Candida parapsilosis Outbreak by FTIR Spectroscopy and STR Analysis

Journal of Fungi, Год журнала: 2024, Номер 10(11), С. 753 - 753

Опубликована: Окт. 30, 2024

Accurate identification and rapid genotyping of

Язык: Английский

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