International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(18), С. 10012 - 10012
Опубликована: Сен. 17, 2024
microRNAs
(miRNAs)
are
promising
biomarkers
for
many
diseases,
including
multiple
sclerosis
(MS).
The
neurofilament
light
chain
(NfL)
is
a
biomarker
that
can
detect
axonal
damage
in
different
neurological
diseases.
objective
of
this
study
was
to
evaluate
the
association
expression
profile
pre-selected
miRNAs
and
NfL
levels
with
clinical
radiological
variables
MS
patients.
We
conducted
1-year
longitudinal
prospective
patients
forms.
measured
disability
using
expanded
status
scale
(EDSS),
magnetic
resonance
imaging
(MRI)
volumetry
baseline,
cognitive
functioning
processing
speed
test
(PST)
at
baseline
1
year
later.
Selected
serum
(sNfL)
were
quantified.
Seventy-three
recruited.
MiR-126.3p
correlated
EDSS
miR-126.3p
miR-9p
deterioration
year.
Correlations
regional
brain
volumes
observed
between
cortical
gray
matter,
cerebellum,
putamen,
pallidum;
miR-146a.5p
cerebellum
miR-29b.3p
white
matter
miR-138.5p
miR-9.5p
thalamus.
sNfL
miR-9.5p.
also
associated
phenotype.
These
data
justify
future
studies
further
explore
utility
(mirR-126.3p,
miR-146.5p,
miR.9-5p)
as
MS.
Cellular and Molecular Neurobiology,
Год журнала:
2024,
Номер
44(1)
Опубликована: Фев. 16, 2024
Abstract
HOX
transcript
antisense
intergenic
RNA
(
HOTAIR
)
is
a
long
non-coding
(lncRNA)
which
increasingly
being
perceived
as
tremendous
molecular
mediator
of
brain
pathophysiology
at
multiple
levels.
Epigenetic
regulation
target
gene
expression
carried
out
by
thorough
modulation
chromatin
modifiers;
histone
methyltransferase
polycomb
repressive
complex
2
(PRC2)
and
demethylase
lysine-specific
1
(LSD1).
Incidentally,
was
the
first
lncRNA
shown
to
elicit
sponging
specific
microRNA
(miRNA
or
miR)
species
in
trans-acting
manner.
It
has
been
extensively
studied
various
cancers,
including
gliomas
regarded
prominent
pro-tumorigenic
pro-oncogenic
lncRNA.
Indeed,
may
serve
glioma
grade
predictor
prognostic
biomarker.
The
objective
this
timely
review
not
only
outline
multifaceted
pathogenic
roles
development
but
also
delineate
research
findings
implicating
it
critical
regulator
overall
pathophysiology.
While
major
focus
on
neuro-oncology,
wherein
represents
particularly
potent
underlying
player
suitable
therapeutic
target,
mechanisms
regulatory
actions
neurodegeneration,
traumatic,
hypoxic
ischemic
injuries,
neuropsychiatric
disorders
are
presented.
Graphical
HOTAIR-mediated
epigenetic
DNA
miRNAs.
5′
end
regulates
H3K27
trimethylation
activity
catalytic
subunit
enhancer
Zeste
homolog
(EZH2)
(PRC2),
its
3′
modulates
H3K4
demethylation
binds
competitively
inhibits
functions
miRNAs,
altering
downstream
genes.
Brain Sciences,
Год журнала:
2024,
Номер
14(4), С. 399 - 399
Опубликована: Апрель 19, 2024
Background:
Ischemic
stroke
(IS)
is
one
of
the
leading
causes
death
and
disability
worldwide.
The
narrow
therapeutic
window
(within
4.5
h)
severe
hemorrhagic
potential
limits
efficacy
recombinant
tissue
type
plasminogen
activator
(rt-PA)
intravenous
thrombolysis
for
patients.
Xingnao
Kaiqiao
(XNKQ)
acupuncture
an
integral
part
traditional
Chinese
medicine,
specifically
designed
to
address
acute
ischemic
by
targeting
key
acupoints
such
as
Shuigou
(GV26)
Neiguan
(PC6).
In
this
study,
we
explored
XNKQ
in
extending
time
interrogated
molecular
mechanisms
responsible
effect.
Methods:
effect
was
evaluated
via
TTC
staining,
neuronal
score
evaluation,
transformation
assay,
H&E
staining.
RNA
sequencing
(RNA-seq)
technology
performed
identify
targets
intervention
acupuncture.
Evans
blue
staining
transmission
electron
microscopy
were
used
assess
blood–brain
barrier
(BBB)
integrity.
Immunofluorescence
co-immunoprecipitation
evaluate
level
autophagy
apoptosis
validate
their
interactions
with
BBB
endothelial
cells.
Results:
Acupuncture
alleviated
infarction
neurological
deficits
extended
6
h.
RNA-seq
revealed
16
predictors
intervention,
which
related
suppressing
inflammation
restoring
function
blood
vessels.
Furthermore,
suppressed
leakage
preserved
tight
junction
protein
expression.
protective
associated
regulation
autophagy–apoptosis
balance
dissociated
Beclin1/Bcl-2
complex,
thereby
promoting
reducing
apoptosis.
Conclusion:
could
serve
adjunctive
therapy
rt-PA
thrombolysis,
aiming
extend
mitigate
ischemia–reperfusion
injury.
disruption
regulating
balance,
turn
IS.
These
findings
provide
a
rationale
further
exploration
complementary
candidate
co-administered
rt-PA.
Brain Research Bulletin,
Год журнала:
2024,
Номер
209, С. 110922 - 110922
Опубликована: Март 6, 2024
Sepsis
causes
significant
morbidity
and
mortality
worldwide,
most
surviving
patients
show
acute
or
chronic
mental
disorders,
which
are
known
as
sepsis-associated
encephalopathy
(SAE).
SAE
involves
many
pathological
processes,
including
the
blood-brain
barrier
(BBB)
damage.
The
BBB
is
located
at
interface
between
central
nervous
system
surrounding
environment,
protects
(CNS)
from
invasion
of
exogenous
molecules,
harmful
substances
microorganisms
in
blood.
Recently,
a
growing
number
studies
have
indicated
that
destruction
was
involved
played
an
important
role
SAE-induced
brain
injury.
In
present
review,
we
firstly
reveal
processes
such
neurotransmitter
oxidative
stress,
immune
dysfunction
destruction.
Moreover,
introduce
structure
BBB,
describe
cells
microglia
astrocytes
participate
after
SAE.
Furthermore,
view
current
research
on
non-coding
RNAs
(ncRNAs),
explain
regulatory
mechanism
ncRNAs
long
noncoding
(lncRNAs),
microRNAs
(miRNAs)
circular
(circRNAs)
Finally,
propose
some
challenges
perspectives
regulating
functions
Hence,
basis
these
effects,
both
may
be
developed
therapeutic
targets
to
protect
for
patients.
Frontiers in Aging Neuroscience,
Год журнала:
2024,
Номер
16
Опубликована: Авг. 26, 2024
The
blood-brain
barrier
is
known
to
consist
of
a
variety
cells
and
complex
inter-cellular
junctions
that
protect
the
vulnerable
brain
from
neurotoxic
compounds;
however,
it
also
complicates
pharmacological
treatment
central
nervous
system
disorders
as
most
drugs
are
unable
penetrate
on
basis
their
own
structural
properties.
This
dramatically
diminished
therapeutic
effect
drug
compromised
its
biosafety.
In
response,
number
often
delivered
lesions
in
invasive
ways
bypass
obstruction
barrier,
such
subdural
administration,
intrathecal
convection-enhanced
delivery.
Nevertheless,
these
intrusive
strategies
introduce
risk
injury,
limiting
clinical
application.
recent
years,
intensive
development
nanomaterials
science
interdisciplinary
convergence
medical
engineering
have
brought
light
penetration
for
brain-targeted
drugs.
this
paper,
we
extensively
discuss
limitations
delivery
non-invasive
nanomedicine
disruption.
meantime,
analyze
strengths
provide
outlooks
further
systems.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Апрель 24, 2025
Subarachnoid
hemorrhage
(SAH)
is
a
disease
with
high
mortality
and
morbidity,
its
pathophysiology
complex
but
poorly
understood.
To
investigate
the
potential
therapeutic
targets
post-SAH,
SAH-related
feature
genes
were
screened
by
combined
analysis
of
transcriptomics
metabolomics
rat
cortical
tissues
following
SAH
proteomics
cerebrospinal
fluid
from
patients,
as
well
WGCNA
machine
learning.
The
competitive
endogenous
RNAs
(ceRNAs)
transcription
factors
(TFs)
regulatory
networks
constructed
further
validated
molecular
biology
experiments.
A
total
1336
differentially
expressed
proteins
identified,
including
729
downregulated
607
upregulated.
immune
microenvironment
changed
after
changement
persisted
at
7d.
Through
multi-omics
bioinformatics
techniques,
five
(A2M,
GFAP,
GLIPR2,
GPNMB,
LCN2)
closely
related
to
microenvironment.
In
addition,
ceRNAs
TFs
constructed.
increased
expression
levels
A2M
GLIPR2
verified,
co-localization
intravascular
microthrombus
was
demonstrated.
Multiomics
tools
used
predict
associated
confirmed
through
network
development.
These
molecules
might
play
key
role
in
may
serve
biological
markers
provide
clues
for
exploring
options.
Genes,
Год журнала:
2024,
Номер
15(4), С. 431 - 431
Опубликована: Март 29, 2024
Many
investigations
have
evaluated
the
expression
of
noncoding
RNAs
(ncRNAs)
as
well
their
related
molecular
functions
and
biological
machineries
in
individuals
with
alcohol
dependence.
Alcohol
dependence
may
be
one
most
prevailing
psychological
disorders
globally,
its
pathogenesis
is
intricate
inadequately
comprehended.
There
substantial
evidence
indicating
significant
links
between
multiple
genetic
factors
development
In
particular,
critical
roles
ncRNAs
been
emphasized
pathology
mental
illnesses,
probably
including
comprehension
action
modification,
furthermore,
they
emerged
therapeutic
targets
for
a
variety
psychiatric
It
worth
mentioning
that
dysregulated
has
regularly
detected
An
in-depth
knowledge
m6A
modification
valuable
novel
treatment
against
general,
more
profound
understanding
practical
might
make
important
contributions
to
precise
diagnosis
and/or
actual
management
Here,
this
review,
we
mostly
focused
on
up-to-date
regarding
alterations
modifications
Then,
present
prospects
future
research
applications
concept
engram
system.
Expert Reviews in Molecular Medicine,
Год журнала:
2024,
Номер
26
Опубликована: Янв. 1, 2024
Abstract
Long
non-coding
RNAs
(lncRNAs)
are
progressively
being
perceived
as
prominent
molecular
agents
controlling
multiple
aspects
of
neuronal
(patho)physiology.
Amongst
these
is
the
HOX
transcript
antisense
intergenic
RNA,
often
abbreviated
HOTAIR
.
epigenetically
regulates
its
target
genes
via
interaction
with
two
different
chromatin-modifying
agents;
histone
methyltransferase
polycomb-repressive
complex
2
and
demethylase
lysine-specific
1.
Parenthetically,
elicits
trans-acting
sponging
function
against
micro-RNA
species.
Oncological
research
studies
have
confirmed
pathogenic
functions
in
cancer
types,
such
gliomas
proposed
it
a
pro-oncological
lncRNA.
In
fact,
expression
has
been
suggested
to
be
predictor
severity/grade
gliomas,
prognostic
biomarker.
Moreover,
propound
influence
other
brain
heath
disease
states
just
beginning
unravelled.
The
objective
this
review
recapitulate
all
relevant
data
pertaining
regulatory
roles
To
end,
we
discuss
mechanisms
diseases,
neurodegeneration,
traumatic
injury
neuropsychiatric
disorders.
Finally,
also
summarize
results
from
incriminating
pathogeneses
cancers.
Implications
serving
suitable
therapeutic
neuropathologies
discussed.
iScience,
Год журнала:
2023,
Номер
26(11), С. 108270 - 108270
Опубликована: Окт. 20, 2023
Fucoxanthin
is
the
most
abundant
marine
carotenoid
extracted
from
seaweed.
Our
previous
study
has
shown
that
fucoxanthin
inhibited
oxidative
stress
after
traumatic
brain
injury
(TBI).
However,
effects
of
on
TBI-induced
blood-brain
barrier
(BBB)
destruction
have
not
been
well
understood.
In
present
study,
we
found
improved
neurological
dysfunction,
reduced
edema,
attenuated
cortical
lesion
volume,
and
decreased
dendrites
loss
TBI
in
vivo.
Moreover,
suppressed
BBB
leakage,
preserved
tight
junction
(TJ)
adherens
(AJ)
proteins,
MMP-9
expression.
Furthermore,
alleviated
apoptosis
ferroptosis,
activated
mitophagy
endothelial
cells
(ECs)
TBI.
protection
was
when
inhibited.
Importantly,
also
provided
protective
bEnd.3
Taken
together,
our
results
suggested
played
a
key
role
through
mitophagy.
A
great
deal
of
studies
have
assessed
the
expression
noncoding
RNAs
(ncRNAs)
as
well
their
relevant
molecular
functions
and
biological
mechanisms
in
individuals
alcohol
dependence.
Alcohol
dependence
is
one
most
prevalent
neuropsychological
disorders
worldwide,
its
pathogenesis
intricate
inadequately
understood.
There
considerable
evidence
demonstrating
substantial
links
between
multiple
genetic
factors
development
Critical
roles
ncRNAs
been
emphasized
pathophysiology
mental
diseases
including
In
comprehension
action
modification,
they
emerged
therapeutic
targets
for
a
variety
psychiatric
illness
Worth
mentioning,
dysregulated
has
regularly
detected
An
in-depth
knowledge
m6A
modification
may
be
valuable
novel
treatment
against
general,
deeper
functional
make
significant
contributions
to
precise
diagnosis
and/or
actual
Here
this
review,
we
mostly
focused
on
up-to-date
regarding
alterations
modifications
dependence,
then,
presented
prospects
future
research
application
with
concept
engram-system.