Isolation and identification of novel selective antitumor constituents, sidrin and sidroside, from Zizyphus spina-christi

Saudi Pharmaceutical Journal, Год журнала: 2023, Номер 31(6), С. 1019 - 1028

Опубликована: Май 6, 2023

The leaves of Zizyphus spina-christi (L.) Willd contain several compounds exhibiting different pharmacologic activities. However, studies on the cytotoxic activity these are limited.We aimed to investigate and isolate with selective antitumor effects from Z. using bioassay-guided fractionation methanol extract.Powdered, dried were subjected extraction fractionated n-hexane, chloroform, ethyl acetate, n-butanol. Fractions positive cytotoxicity against HeLa THP-1 cell lines further eluted various concentrations organic solvents. Active isolated chromatographic methods their chemical structures determined extensive spectroscopic methods, such as 1D NMR (1H NMR, 13C DEPT), 2D (COSY, HMBC, HMQC), HRFAB-MS, IR. Furthermore, evaluated 62 tumor (including THP-1) in addition normal bone marrow cells.The chloroform aqueous fractions showed activity. Two successfully named "sidrin" (13-β-hydroxy-lup-20(30)-ene-2,3-β-epoxy-28-carboxylate) "sidroside" (3-O-β-D-glucopyranosyl-(1-3)-α-L-arabinopyranosyl-jujubogenin-20-O-α-L-rhamnopyranoside). Sidrin exhibited human leukemia (Hl-60, RPMI-8226), lung cancer (A549, EKVX), breast (BT-549, MDA-MB-231/ATCC), colon (KM12), melanoma (M14, SK-MEL-5), central nervous system (CNS) (SF-295) lines, selectivity was observed Hl-60, EKVX, BT-549, KM12, SF-295 lines. In addition, sidrin more active than sidroside doxorubicin Hl-60 EKVX contrast, had a similar effect BT-549 renal (UO-31) Sidroside (CCRF-CEM, MOLT-4), (HOP-92, NCI-H322M), (MDA-MB-468), (LOX IMVI), CNS (SNB-19), ovarian (OVCAR-8), (UO-31, RXF 393), prostate (PC-3) Both (MDA-MB-231, T-47D), (HCC-2998, HCT-116), (OVCAR-3), 786-0, SN 12C) Normal cells unaffected at same applied cells.These results suggest tumor-selective sidroside.

Язык: Английский

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Saponin Fractions from Eryngium planum L. Induce Apoptosis in Ovarian SKOV-3 Cancer Cells

Plants, Год журнала: 2023, Номер 12(13), С. 2485 - 2485

Опубликована: Июнь 29, 2023

(1) The cytotoxicity and antioxidant activity of different fractions as well the pro-apoptotic saponin from Eryngium planum L. in SKOV-3 was investigated. (2) In screening studies, six on examined by LDH SRB assays. most active fractions—triterpenoid saponins—were selected for further investigation. To determine mechanism fractions’ cytotoxicity, their ability to induce apoptosis via Annexin V assay. effect caspase 3 measured using a Caspase Assay Kit. expression 84 apoptosis-related genes investigated cancer cells exposed roots. radical scavenging capacity determined DPPH (3) pronounced cytotoxic effects were demonstrated leaves Those chosen treatment cell lines with saponins obtained roots provoked significant increase apoptotic cells. cells, caused upregulation decrease anti-apoptotic genes. activation correlated an increased DFFA level treated phenolic acids shoots (4) best our knowledge, current study is first demonstrate that barrigenol-type triterpenoid fraction E. inhibits proliferation induces apoptosis, which may be regulated mostly specific mitochondria-related pathway.

Язык: Английский

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NMR-guided isolation of undescribed triterpenoid saponins from Lysimachia atropurpurea L

Phytochemistry, Год журнала: 2024, Номер 223, С. 114104 - 114104

Опубликована: Апрель 23, 2024

Язык: Английский

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COMPARATIVE STUDY OF BIOCHEMICAL COMPOUNDS AND ITS RELATED THERAPEUTIC EFFICACY OF IPHIONA MUCRONATA AND CONYZA DIOSCORIDES

Egyptian Journal of Desert Research, Год журнала: 2024, Номер 74(1), С. 129 - 147

Опубликована: Май 25, 2024

The purpose of this study was to compare the biological effects as antimicrobial and antitumor activity bioactive ingredients two plants Iphonia mucronata Conyza dioscorides which belong Asteraceae family. I. EtOAc extract exhibited promising against HELA (cervical), PC-3 (prostate), HEP-2 (larynx) carcinoma cell lines with IC50 values 24.60, 26.57 39.06 μg/ml, respectively. While C. showed weak moderate same lines. MeOH extracts activity, also antibacterial Proteus vulgaris, Bacillus subtilis Escherichia coli, total active constituents both revealed higher concentrations than all components except alkaloid content. phenolic, flavonoid, were in while saponins content whereas tannins similar for plants. major phenolic acids flavonoid compounds investigated using HPLC caffeic, ferulic acid, naringin, luteolin mucronata.

Язык: Английский

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Early developmental anomalies in zebrafish (Danio rerio) embryos induced by the Clematis florida Thunb.

Journal of Ethnopharmacology, Год журнала: 2024, Номер unknown, С. 118897 - 118897

Опубликована: Окт. 1, 2024

Язык: Английский

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