Root zone microbial communities of Artemisia ordosica Krasch. at different successional stages in Mu US Sandy Land: a metagenomic perspective with culturomics insights
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Май 9, 2025
Phytoremediation
offers
a
promising
strategy
for
addressing
the
global
challenge
of
land
desertification.
In
Mu
Us
Sandy
Land
China,
Artemisia
ordosica
Krasch.
has
emerged
as
key
species
desertification
control.
Its
root-associated
microbial
communities
may
enhance
plant’s
adaptability
to
sandy,
nutrient-poor
environments.
Despite
their
ecological
significance,
comprehensive
investigations
these
remain
limited.
this
study,
in
root
zone
(i.e.,
rhizosphere
soil,
non-rhizosphere
and
endosphere)
A.
were
analyzed
via
high-throughput
sequencing
different
isolation
approaches
across
successional
stages
(moving
dunes,
semi-fixed
fixed
dunes)
northern
China.
Metagenomic
analysis
revealed
that
diversity
was
significantly
higher
soils
than
endosphere;
moving
dunes
exhibited
lower
dunes.
Meanwhile,
distinct
community
structures
by
principal
coordinates
(PCoA),
demonstrating
substantial
differences
between
endosphere
other
zones.
Environmental
factors,
including
nitrate
nitrogen
(NO
3
−
-N),
organic
matter
(OM),
available
potassium
(AK),
total
(TK),
influenced
composition.
Moreover,
dominant
genera
such
Arthrobacter
Paraphoma
identified,
potentially
contributing
growth.
From
culturomics
perspective,
93
bacterial
isolates
obtained
using
conventional
streak
plate
colony
pick
methods,
with
Firmicutes
(37.63%)
Bacillus
(23.66%)
identified
taxa.
parallel,
14
fungal
strains
isolated,
primarily
belonging
Penicillium
(35.71%)
Aspergillus
(21.43%),
both
which
are
well-documented
stress
tolerance
arid
ecosystems.
A
cultivation
identification
method,
tailored
recover
rare
slow-growing
bacteria,
employed
successfully
broadened
cultured
include
Proteobacteria
(46.43%)
representatives
rarely
cultivated
Deinococcus-Thermus
phylum.
This
study
provides
metagenomic
insights
into
associated
,
enhancing
understanding
plant–microbe
interactions
sandy
Язык: Английский
Cellular Mechanical Phenotypes of Drought-Resistant and Drought-Sensitive Rice Species Distinguished by Double-Resonator Piezoelectric Cytometry Biosensors
Biosensors,
Год журнала:
2025,
Номер
15(6), С. 334 - 334
Опубликована: Май 23, 2025
Various
high-throughput
screening
methods
have
been
developed
to
explore
plant
phenotypes,
primarily
at
the
organ
and
whole
levels.
There
is
a
need
develop
phenomics
cellular
level
narrow
down
genotype
phenotype
gap.
This
study
used
double-resonator
piezoelectric
cytometry
biosensors
capture
dynamic
changes
in
mechanical
phenotypes
of
living
cells
two
rice
species,
drought-resistant
Lvhan
No.
1
drought-sensitive
6527,
under
PEG6000
drought
stress.
In
mechanomics
parameters,
including
cell-generated
surface
stress
(ΔS)
viscoelastic
parameters
(G′,
G″,
G″/G′),
were
measured
compared
5–25%
PEG6000.
showed
larger
but
smaller
with
same
concentration
Moreover,
better
wall–plasma
membrane–cytoskeleton
continuum
structure
maintaining
ability
stress,
as
proven
by
transient
tension
(ΔS
>
0)
linear
G′~ΔS,
G″~ΔS
relations
higher
15–25%
PEG6000,
not
for
6527
cells.
Additionally,
distinct
defense
resistance
mechanisms
identified
through
G″/G′
responses:
(i)
hardening
followed
softening
recovery
weak
drought,
(ii)
strong
drought.
The
abilities
both
recover
from
are
than
those
Overall,
phenotypic
patterns
(ΔS,
G′,
G″/G′,
G″~ΔS)
verified
that
has
which
consistent
field
data.
Язык: Английский