Root zone microbial communities of Artemisia ordosica Krasch. at different successional stages in Mu US Sandy Land: a metagenomic perspective with culturomics insights DOI Creative Commons

Wen Zhu,

Shuo Han, Yao Cheng

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 9, 2025

Phytoremediation offers a promising strategy for addressing the global challenge of land desertification. In Mu Us Sandy Land China, Artemisia ordosica Krasch. has emerged as key species desertification control. Its root-associated microbial communities may enhance plant’s adaptability to sandy, nutrient-poor environments. Despite their ecological significance, comprehensive investigations these remain limited. this study, in root zone (i.e., rhizosphere soil, non-rhizosphere and endosphere) A. were analyzed via high-throughput sequencing different isolation approaches across successional stages (moving dunes, semi-fixed fixed dunes) northern China. Metagenomic analysis revealed that diversity was significantly higher soils than endosphere; moving dunes exhibited lower dunes. Meanwhile, distinct community structures by principal coordinates (PCoA), demonstrating substantial differences between endosphere other zones. Environmental factors, including nitrate nitrogen (NO 3 − -N), organic matter (OM), available potassium (AK), total (TK), influenced composition. Moreover, dominant genera such Arthrobacter Paraphoma identified, potentially contributing growth. From culturomics perspective, 93 bacterial isolates obtained using conventional streak plate colony pick methods, with Firmicutes (37.63%) Bacillus (23.66%) identified taxa. parallel, 14 fungal strains isolated, primarily belonging Penicillium (35.71%) Aspergillus (21.43%), both which are well-documented stress tolerance arid ecosystems. A cultivation identification method, tailored recover rare slow-growing bacteria, employed successfully broadened cultured include Proteobacteria (46.43%) representatives rarely cultivated Deinococcus-Thermus phylum. This study provides metagenomic insights into associated , enhancing understanding plant–microbe interactions sandy

Language: Английский

Root zone microbial communities of Artemisia ordosica Krasch. at different successional stages in Mu US Sandy Land: a metagenomic perspective with culturomics insights DOI Creative Commons

Wen Zhu,

Shuo Han, Yao Cheng

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 9, 2025

Phytoremediation offers a promising strategy for addressing the global challenge of land desertification. In Mu Us Sandy Land China, Artemisia ordosica Krasch. has emerged as key species desertification control. Its root-associated microbial communities may enhance plant’s adaptability to sandy, nutrient-poor environments. Despite their ecological significance, comprehensive investigations these remain limited. this study, in root zone (i.e., rhizosphere soil, non-rhizosphere and endosphere) A. were analyzed via high-throughput sequencing different isolation approaches across successional stages (moving dunes, semi-fixed fixed dunes) northern China. Metagenomic analysis revealed that diversity was significantly higher soils than endosphere; moving dunes exhibited lower dunes. Meanwhile, distinct community structures by principal coordinates (PCoA), demonstrating substantial differences between endosphere other zones. Environmental factors, including nitrate nitrogen (NO 3 − -N), organic matter (OM), available potassium (AK), total (TK), influenced composition. Moreover, dominant genera such Arthrobacter Paraphoma identified, potentially contributing growth. From culturomics perspective, 93 bacterial isolates obtained using conventional streak plate colony pick methods, with Firmicutes (37.63%) Bacillus (23.66%) identified taxa. parallel, 14 fungal strains isolated, primarily belonging Penicillium (35.71%) Aspergillus (21.43%), both which are well-documented stress tolerance arid ecosystems. A cultivation identification method, tailored recover rare slow-growing bacteria, employed successfully broadened cultured include Proteobacteria (46.43%) representatives rarely cultivated Deinococcus-Thermus phylum. This study provides metagenomic insights into associated , enhancing understanding plant–microbe interactions sandy

Language: Английский

Citations

0