Engineered polyethylene terephthalate hydrolases: perspectives and limits

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Июль 2, 2024

Abstract Polyethylene terephthalate (PET) is a major component of plastic waste. Enzymatic PET hydrolysis the most ecofriendly recycling technology. The biorecycling waste requires complete depolymerization to and ethylene glycol. history enzymatic has revealed two critical issues for industrial PET: industrially available hydrolases pretreatment make it susceptible full hydrolysis. As none wild-type enzymes can satisfy requirements industrialization, various mutational improvements have been performed, through classical technology state-of-the-art computational/machine-learning Recent engineering studies on brought new insight that flexibility substrate-binding groove may improve efficiency while maintaining sufficient thermostability, although previous focused only thermostability above glass transition temperature PET. Industrial scheduled be implemented, using micronized amorphous Next stage must development efficiently degrade crystalline parts expansion target materials, not bottles but also textiles, packages, microplastics. This review discusses current status hydrolases, their potential applications, profespectal goals. Key points • thermophilic, operation below 70 °C Classical approaches are useful Enzyme activity expected future Graphical

Язык: Английский

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Unusual depolymerization mechanism of Poly(ethylene terephthalate) by hydrolase 202

Chemosphere, Год журнала: 2025, Номер 372, С. 144108 - 144108

Опубликована: Янв. 15, 2025

Язык: Английский

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Protein Engineering for Industrial Biocatalysis: Principles, Approaches, and Lessons from Engineered PETases

Catalysts, Год журнала: 2025, Номер 15(2), С. 147 - 147

Опубликована: Фев. 4, 2025

Protein engineering has emerged as a transformative field in industrial biotechnology, enabling the optimization of enzymes to meet stringent demands for stability, specificity, and efficiency. This review explores principles methodologies protein engineering, emphasizing rational design, directed evolution, semi-rational approaches, recent integration machine learning. These strategies have significantly enhanced enzyme performance, even rendering engineered PETase industrially relevant. Insights from PETases underscore potential tackle environmental challenges, such advancing sustainable plastic recycling, paving way innovative solutions biocatalysis. Future directions point interdisciplinary collaborations emerging learning technologies revolutionize design.

Язык: Английский

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Perspectives on the microorganisms with the potentials of PET-degradation

Frontiers in Microbiology, Год журнала: 2025, Номер 16

Опубликована: Март 12, 2025

Polyethylene terephthalate (PET), a widely used synthetic polymer in daily life, has become major source of post-consumer waste due to its complex molecular structure and resistance natural degradation, which posed significant threat the global ecological environment human health. Current PET-processing methods include physical, chemical, biological approaches, however each have their limitations. Given that numerous microbial strains exhibit remarkable capacity degrade plastic materials, degradation PET emerged as highly promising alternative. This approach not only offers possibility converting into valuable resources but also contributes advancement circular economy. Therefore this review, it is mainly focused on cutting-edge technologies key role specific such Ideonella sakaiensis 201-F6, can efficiently assimilate PET. Particularly noteworthy are catalytic enzymes related metabolism PET, been emphasized sustainable eco-friendly strategy for recycling within framework Furthermore, study elucidates innovative utilization degraded materials feedstock production high-value chemicals, highlighting path forward management waste.

Язык: Английский

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Efficient and Selective Catalytic Oxidation of Ethylbenzene over Co-MOF Synthesized from Waste PET

Industrial & Engineering Chemistry Research, Год журнала: 2025, Номер unknown

Опубликована: Март 24, 2025

Язык: Английский

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Harnessing the power of microbial consortia for the biodegradation of per- and polyfluoroalkyl substances: Challenges and opportunities

Chemosphere, Год журнала: 2025, Номер 374, С. 144221 - 144221

Опубликована: Фев. 21, 2025

Язык: Английский

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Synergistic functional activity of a landfill microbial consortium in a microplastic-enriched environment

The Science of The Total Environment, Год журнала: 2024, Номер 947, С. 174696 - 174696

Опубликована: Июль 10, 2024

Plastic pollution of the soil is a global issue increasing concern, with far-reaching impact on environment and human health. To fully understand medium- long-term plastic dispersal in environment, it necessary to define its interaction residing microbial communities biochemical routes degradation metabolization. However, despite recent attention this problem, research has largely focussed functional potential, failing clearly identify collective adaptation strategies these communities. Our study combines genome-centric metagenomics metatranscriptomics characterise adapting high polyethylene terephthalate concentration. The microbiota were sampled from landfill subject decades-old contamination enriched through prolonged cultivation using microplastics as only carbon source. This approach aimed select microorganisms that best adapt specific substrates. As result, we obtained simplified where multiple metabolization pathways are widespread across abundant rare taxa. Major differences found terms expression, which average was higher planktonic microbes than those firmly adhered plastic, indicating complementary metabolic roles potential microplastic assimilation. Moreover, metatranscriptomic patterns indicate transcriptional level numerous genes emerging taxa characterised by marked accumulation genomic variants, supporting hypothesis requires an extensive rewiring energy metabolism thus provides strong selective pressure. Altogether, our results provide improved characterisation derived common plastics types terrestrial suggest biotic responses investing contaminated sites well biotechnological targets for cooperative upcycling.

Язык: Английский

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Magnetic silica-coated cutinase immobilized via ELPs biomimetic mineralization for efficient nano-PET degradation

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 279, С. 135414 - 135414

Опубликована: Сен. 6, 2024

Язык: Английский

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Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes

Microbial Cell Factories, Год журнала: 2024, Номер 23(1)

Опубликована: Окт. 10, 2024

With a growing global population, the generation of plastic waste and depletion fossil resources are major concerns that need to be addressed by developing sustainable efficient recycling methods. Biocatalytic is emerging as promising ecological alternative conventional processes, particularly in polyethylene terephthalate (PET). However, cost-effective production involved biocatalyst essential for transition enzymatic PET widely used industrial technology. Extracellular enzyme using established organisms such Escherichia coli or Corynebacterium glutamicum offers way reduce downstream processing costs.

Язык: Английский

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Fusion of Hydrophobic Anchor Peptides Promotes the Hydrolytic Activity of PETase but not the Extent of PET Depolymerization

ChemCatChem, Год журнала: 2024, Номер unknown

Опубликована: Окт. 28, 2024

Abstract Enzymatic recycling of polyethylene terephthalate (PET) has attracted significant attention in recent years. While the fusion anchor peptides to PET hydrolases is believed enhance hydrolytic activity, a quantitative analysis yet lacking. Here, we construct four enzymes by fusing (including hydrophobic LCI, LCIM1 and TA2, hydrophilic EK4) C terminus HotPETase, one most active for high‐crystallinity (HC‐PET). Single‐molecule force spectroscopy (SMFS) demonstrates that promote adhesive interactions between surface. This also validated adsorption kinetics isotherms, saturated capacity remains unaltered compared HotPETase. At low substrate loadings, apparent activity these positively related hydrophobicity peptides. Among them, HotPETase‐LCI stands out as effective enzyme HC‐PET degradation, demonstrating 1.5‐fold increase activity. high advantages with diminish. We conclude only facilitate rates reactions but have little effect on final conversion extent.

Язык: Английский

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