Microfluidic programmable strategies for channels and flow

Lab on a Chip, Год журнала: 2024, Номер 24(19), С. 4483 - 4513

Опубликована: Янв. 1, 2024

This review summarizes programmable microfluidics, an advanced method for precise fluid control in microfluidic technology through microchannel design or liquid properties, referring to microvalves, micropumps, digital multiplexers, micromixers, slip-, and block-based configurations. Different microvalve types, including electrokinetic, hydraulic/pneumatic, pinch, phase-change check valves, cater diverse experimental needs. Programmable such as passive active play a crucial role achieving automation. Due their small size high integration, microvalves micropumps are widely used medical devices biological analysis. In addition, this provides in-depth exploration of the applications multiplexed mixer-based microfluidics manipulation movement, mixing, splitting. These methodologies leverage physical properties liquids, capillary forces dielectric forces, achieve over dynamics. SlipChip technology, which branches into rotational translational SlipChip, controls sliding motion microchannel. On other hand, innovative designs systems pursue better modularity, reconfigurability ease assembly. assembly strategies, from one-dimensional blocks two-dimensional Lego®-style three-dimensional reconfigurable modules, aim enhance flexibility accessibility. technologies user-friendliness accessibility by offering integrated systems, making them potentially usable outside specialized technical labs. Microfluidic strategies channels flow hold promising biomedical research, chemical analysis drug screening, providing theoretical practical guidance broader utilization scientific research applications.

Язык: Английский

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The microbiota: a key regulator of health, productivity, and reproductive success in mammals

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Ноя. 5, 2024

The microbiota, intensely intertwined with mammalian physiology, significantly impacts health, productivity, and reproductive functions. normal microbiota interacts the host through following key mechanisms: acting as a protective barrier against pathogens, maintain mucosal integrity, assisting in nutrient metabolism, modulating of immune response. Therefore, supporting growth development host, providing protection pathogens toxic substances. influences brain behavior, demonstrated by comprehensive findings from controlled laboratory experiments human clinical studies. prospects suggested that gut microbiome influence neurodevelopmental processes, modulate stress responses, affect cognitive function gut-brain axis. Microbiota gastrointestinal tract farm animals break down ferment ingested feed into nutrients, utilize to produce meat milk. Among beneficial by-products short-chain fatty acids (SCFAs) are particularly noteworthy for their substantial role disease prevention promotion various productive aspects mammals. plays pivotal hormonal systems mammals, boosting performance both sexes fostering maternal-infant connection, thereby becoming crucial factor sustaining existence. is critical influencing success production traits A well-balanced improves absorption metabolic efficiency, leading better rates, increased milk production, enhanced overall health. Additionally, it regulates hormones like estrogen progesterone, which essential successful conception pregnancy. Understanding offers valuable insights optimizing breeding improving outcomes, contributing advancements agriculture veterinary medicine. This study emphasizes ecological roles highlighting contributions success. By integrating perspectives, demonstrates how microbial communities enhance function, regulation across species, offering benefit agricultural advancements.

Язык: Английский

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Impact of Glyphosate on Soil Bacterial Communities and Degradation Mechanisms in Large-Leaf Tea Plantations

Journal of Hazardous Materials, Год журнала: 2024, Номер 483, С. 136626 - 136626

Опубликована: Ноя. 22, 2024

Язык: Английский

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Genome Sequencing Reveals the Potential of Enterobacter sp. Strain UNJFSC003 for Hydrocarbon Bioremediation

Genes, Год журнала: 2025, Номер 16(1), С. 89 - 89

Опубликована: Янв. 16, 2025

Bioremediation induced by bacteria offers a promising alternative for the contamination of aromatic hydrocarbons due to their metabolic processes suitable removal these pollutants, as many them are carcinogenic molecules and dangerous human health. Our research focused on isolating bacterium from rhizosphere tara tree with ability degrade polycyclic hydrocarbons, using draft genomic sequencing computational analysis. Enterobacter sp. strain UNJFSC 003 possesses 4460 protein-coding genes, two rRNA 77 tRNA GC content 54.38%. A taxonomic analysis our revealed that it has an average nucleotide identity (ANI) 87.8%, indicating is new native Enterobacteria. Additionally, pangenomic 15 strains demonstrated phylogenetic relationship FDAARGOS 1428 (Enterobacter cancerogenus), total 381 core genes 4778 accessory genes. Orthologous methods predicted potential use in hydrocarbon bioremediation. Genes were sub-pathways degradation homoprotocatechuate phenylacetate, primarily located cytoplasm. Studies conducted through molecular modeling docking affinity proteins benzo[a]pyrene sub-pathway, specifically hpcB, which enzymatic activity dioxygenase, hpcC, functions aldehyde dehydrogenase. This study provides information Lomas de Lachay capabilities bioremediation other compounds.

Язык: Английский

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Effects of microbial organic fertilizers on soil microbial communities and physicochemical properties in tobacco cultivation

Frontiers in Environmental Science, Год журнала: 2025, Номер 13

Опубликована: Март 26, 2025

Introduction: Tobacco, as an economic crop in our country, not only requires changes cultivation practices but also benefits from reasonable fertilization to enhance yield and quality. Microbial organic fertilizers (MOFs) have received considerable attention recent years; however, the relationship between rhizosphere soil microorganisms of tobacco plants physicochemical properties with MOFs remains poorly understood. Methods: This study established experimental control groups, employing a five-point sampling method collect samples leaves at four different growth stages. High-throughput 16S rRNA gene sequencing was conducted analyze microbial community structure, while physical characteristics were examined. Results Discussion: The results indicated that MOF reduced uptake ammonium nitrogen by stages, promoted dispersion community, significantly altered structure. At phylum level, Actinobacteriota Proteobacteria are first second dominant bacterial phyla community. Significantly, during prosperous stage tobacco, relative abundance (14.90%) group lower than (19.87%), whereas group, (22.99%) higher (22.06%). Furthermore, assessment leaf morphology, yield, quality demonstrated application resulted 21.93% increase 27.38% value per unit area. nicotine content slightly decreased. In summary, this demonstrates can improve communities leaves, providing valuable insights into effects on altering improving

Язык: Английский

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Characteristics of Bacterial Community Structure and Function in Artificial Soil Prepared Using Red Mud and Phosphogypsum

Microorganisms, Год журнала: 2024, Номер 12(9), С. 1886 - 1886

Опубликована: Сен. 13, 2024

The preparation of artificial soil is a potential cooperative resource utilization scheme for red mud and phosphogypsum on large scale, with low cost simple operation. characteristics the bacterial community structure function in three soils were systematically studied first time. Relatively rich communities formed soils, relatively high abundances phyla (e.g.,

Язык: Английский

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Investigation of Cd and Pb enrichment capacities of Erigeron sumatrensis across three polluted regions: Insights into soil parameters and microbial communities

Environmental Research, Год журнала: 2024, Номер 262, С. 119868 - 119868

Опубликована: Авг. 30, 2024

Язык: Английский

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Effects of Trichoderma harzianum Fertilizer on Growth and Rhizosphere Microbial Community of Continuous Cropping Lagenaria siceraria

Microorganisms, Год журнала: 2024, Номер 12(10), С. 1987 - 1987

Опубликована: Сен. 30, 2024

This study analyzed the effects of

Язык: Английский

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Microstructural Evaluation and Linkage to the Engineering Properties of Metal-Ion-Contaminated Clay

Materials, Год журнала: 2024, Номер 17(21), С. 5320 - 5320

Опубликована: Окт. 31, 2024

The rapid progress of urbanization and industrialization has led to the accumulation large amounts metal ions in environment. These are adsorbed onto negatively charged surfaces clay particles, altering total surface charge, double-layer thickness, chemical bonds between which turn affects interactions them. This causes changes microstructure, such as particle rearrangement pore morphology adjustments, ultimately mechanical behavior soil reducing its stability. study explores effects four common ions, including monovalent alkali (Na

Язык: Английский

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The Effect of Bacillus velezensis LJ02 Compounded with Different Fungi on the Growth of Watermelon Seedlings and Microbial Community Structure

Horticulturae, Год журнала: 2024, Номер 10(3), С. 236 - 236

Опубликована: Фев. 28, 2024

The application of beneficial microbial consortium can effectively improve plant disease resistance and its growth. Various fungi were compounded with Bacillus velezensis LJ02 applied to watermelon plants in this paper. results showed that the T2 (compounded Aspergillus aculeatus 9) control gummy stem blight powdery mildew watermelon, while effect reached 83.56% 70.93%, respectively (p < 0.05). Compound treatment improved diversity richness rhizosphere community structure, relative abundance Caulobacterales Xanthomonadaceae significantly increased after applying soil. Meanwhile, internode length was decreased 28% 0.05), maximum leaf 10.33% In addition, delays maturity vegetables. By studying effects on seedlings, our study provides a theoretical basis for popularization compound inoculant.

Язык: Английский

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