Application and development of biosensing strategies for the analysis of the activity of the tumour-associated enzyme FEN1
Talanta,
Год журнала:
2025,
Номер
286, С. 127527 - 127527
Опубликована: Янв. 7, 2025
Язык: Английский
Rapid Detection of microRNA-122 in Serum and Finger Blood Using a Lateral Flow Nucleic Acid Biosensor
Biosensors,
Год журнала:
2025,
Номер
15(1), С. 58 - 58
Опубликована: Янв. 17, 2025
MicroRNA122
(miR-122)
is
a
microRNA
that
highly
expressed
in
hepatocytes
and
has
been
identified
as
prospective
therapeutic
target
biomarker
for
liver
injury.
An
expanding
body
of
research
demonstrated
miR-122
critical
regulator
both
the
initiation
progression
wide
range
diseases.
Traditional
methods
detecting
mainly
include
Northern
blotting
qRT-PCR,
but
they
are
technically
complex
cumbersome,
requiring
expensive
instruments
high
technical
requirements.
In
this
paper,
we
present
novel
rapid
testing
method
utilizing
lateral
flow
nucleic
acid
biosensor
(LFNAB)
sensitive
time-efficient
detection
miR-122.
This
approach
offers
several
advantages,
including
specificity
miR-122,
ability
to
detect
low
concentrations
molecule,
significantly
reduced
time
compared
conventional
methods.
study,
thiol-modified
single-stranded
DNA
probe
(Det-DNA),
biotinylated
capture
(Cap-DNA),
control
(Con-DNA)
used
construct
LFNAB.
A
gold
nanoparticle
(AuNP)
colored
tag,
which
label
Det-DNA
probe.
The
principle
based
on
dual
DNA-miRNA
hybridization
reactions
LFNAB
form
sandwich-type
AuNP-Det-DNA-miR-122-Cap-DNA
complexes,
captured
test
area
visualization
quantification.
After
systematic
optimization
conditions
experiment,
response
was
linear
within
scope
0
pM-100
pM
limit
15
min
3.90
pM.
use
serum
fingertip
blood
yielded
satisfactory
results.
successful
application
indicates
effectiveness
samples,
showcasing
its
potential
utility
clinical
settings
assessing
levels
different
biological
samples.
Язык: Английский
Bioconvergence: advancing biosensors with nanotechnology for real-time health monitoring
Bulletin of the National Research Centre/Bulletin of the National Research Center,
Год журнала:
2025,
Номер
49(1)
Опубликована: Фев. 14, 2025
Abstract
Background
Biosensors
are
tools
that
products
of
amalgamation
biological
constituents
and
physicochemical
elements
so
as
to
detect
analytes,
based
on
signal
production
which
is
measurable.
Thus
here
we
can
see
an
assimilation
biology
microelectronics.
Keeping
a
track
biochemical
processes
in
human
system
essential;
thus,
there
increasing
need
for
extremely
sensitive
economical
devices
make
precise
diagnosis
personalised
medication
treatment
possible.
Objective
This
review
focuses
upon
the
novel
innovations
field
biosensors,
namely
making
use
nanotechnology
form
nanomaterials
such
graphene,
gold
nanoparticles,
quantum
dots,
sulphur-containing
nanomaterials.
Method
A
thorough
evaluation
literature
was
done.
Databases
like
SciFinder,
ScienceDirect,
PubMed,
Google,
Google
Scholar,
Egyptian
Knowledge
Bank
were
used
find
pertinent
publications.
More
than
500
articles
databases
studied
constitute
this
paper.
The
accuracy
retrieved
data
carefully
reviewed
cross-checked.
Selection
nanomaterial
creation
biosensors
studied:
Nanomaterials
(such
carbon
nanotubes,
dots)
according
their
special
qualities
high
surface
area,
biocompatibility,
sensitivity)
selected.
study
biomolecular
integration
done:
To
gain
knowledge
selective
target
integrate
particular
biomolecules
enzymes,
aptamers,
antibodies)
onto
also
manufacturing
sensors
utilised;
sophisticated
methods
self-assembly
lithography)
build
have
exact
control
over
arrangement
studied.
Signal
Transduction:
transform
interactions
into
quantifiable
signals,
effectively
develop
transduction
systems
(e.g.
electrochemical,
optical,
mechanical).
Real-time
Monitoring:
For
ongoing,
non-invasive
health
monitoring,
include
with
wearable
technology
or
point-of-care
diagnostics.
Data
analysis:
interpret
analyse
biosensor
signals
rapid
evaluations,
apply
machine
learning
techniques
analysis
algorithms.
Result
After
applying
article
selection
criteria
reviewing
quality
methodology,
total
200
selected
be
included
review.
Conclusion
combination
nanotechnology,
"bioconvergence",
has
enormous
potential
healthcare.
real-time,
monitoring
built
by
utilising
distinct
specificity
biomolecules.
could
lead
more
efficient
individualised
healthcare
improving
disease
diagnosis,
treatment,
overall
patient
outcomes.
Язык: Английский
Epigenetic insights of microRNAs: Pioneering cancer detection via advanced biosensors
International review of cell and molecular biology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Язык: Английский
Microalgae in health care and functional foods: β-glucan applications, innovations in drug delivery and synthetic biology
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Март 4, 2025
Microalgae
are
emerging
as
a
key
player
in
healthcare,
functional
foods,
and
sustainable
biotech
due
to
their
capacity
produce
bioactive
compounds
like
β-glucans,
omega-3
fatty
acids,
antioxidants
an
eco-friendly
manner.
This
review
comprehensively
discusses
the
role
of
microalgae
healthcare
focusing
particularly
on
β-glucan
therapeutics,
drug
delivery
innovations,
synthetic
biology
applications.
In
microalgae-derived
show
immense
promise
for
treating
diseases,
boosting
immunity,
tackling
oxidative
stress.
Euglena
-derived
paramylon,
type
β-glucan,
has
shown
potential
various
medical
applications,
including
immunomodulation
anticancer
therapy.
Synthetic
bioprocess
engineering
enhancing
microalgae’s
therapeutic
nutritional
value,
with
applications
personalized
medicine.
To
maximize
microalgae,
further
research
development
needed
address
scalability,
regulatory
alignment,
consumer
acceptance,
focus
interdisciplinary
collaboration
practices
align
innovation
environmental
conservation.
Язык: Английский
Revolutionary advances in hypertension detection: Gold nanoparticle-enhanced miRNA-based electrochemical biosensors and emerging nanotechnologies
Human Gene,
Год журнала:
2025,
Номер
unknown, С. 201401 - 201401
Опубликована: Март 1, 2025
Язык: Английский
The Potential of Optical Technologies in Early Virus Detection; Prospects in Addressing Future Viral Outbreaks
Critical Reviews in Analytical Chemistry,
Год журнала:
2025,
Номер
unknown, С. 1 - 29
Опубликована: Март 27, 2025
The
urgent
need
for
sensitive,
rapid,
and
reliable
diagnostic
methodologies
to
control
prevent
life-threatening
pandemic
infectious
disease,
such
as
COVID-19,
remains
a
critical
priority.
Timely
on-site
detection
of
viral
pathogens
is
essential
effective
disease
management
mitigation
societal
disruptions.
Recent
advancements
in
optical
methods
have
positioned
them
at
the
forefront
healthcare
diagnostics,
offering
high
sensitivity
specificity
viable
alternatives
conventional
techniques
Polymerase
Chain
Reaction
(PCR),
which
often
suffer
from
time
delays
limited
accessibility
resource-constrained
environments.
This
review
elucidates
potential
various
techniques,
highlighting
their
advantages
over
traditional
methods.
It
encompasses
range
modalities,
including
fluorescence-based
approaches,
Raman
spectroscopy
(RS),
Plasmonic
(e.g.,
surface
plasmon
resonance
(SPR),
localized
SPR,
(LSPR),
surface-enhanced
(SERS),
fluorescence
(SEF)),
super
resolution
microscopies
(SRMs),
attenuated
total
reflectance-Fourier
transform
infrared
(ATR-FTIR),
integrated
platforms
waveguides
molecularly
imprinted
polymer
(MIP)-based
biosensors.
Additionally,
evolution
novel
biosensors,
particularly
5th
6th
generation
challenges
related
these
technologies
were
discussed.
studies
reviewed
aims
advance
development
portable,
specific,
cost-effective
point-of-care
(POC)
devices
rapid
pathogens.
Язык: Английский
Advancing gut microbiome insights: Graphene quantum dot nanobiosensors for microbial metabolite detection
Chemical Engineering Journal,
Год журнала:
2025,
Номер
unknown, С. 162954 - 162954
Опубликована: Апрель 1, 2025
A Novel Microelectrode Based on Joule Heating and Impedance Spectroscopy for Inducing and Monitoring the Aggregation of HCV-Specific Probes
Sensors,
Год журнала:
2025,
Номер
25(11), С. 3312 - 3312
Опубликована: Май 24, 2025
The
world
urgently
needs
new
methods
to
quickly
and
efficiently
detect
mutated
viruses.
An
RNA-AuNP-based
colorimetric
biosensor
is
a
highly
sensitive,
specific,
cost-effective
tool
that
enables
rapid,
visual
detection
of
target
molecules
for
applications
in
disease
diagnostics,
environmental
monitoring,
forensic
analysis.
requires
precise
control
over
nanoparticle
dispersion
aggregation,
which
can
be
achieved
using
temperature
regulation.
A
novel
on-chip
microelectrode
proposed
induce
monitor
the
aggregation
RNA-attached
gold
nanoparticles
(AuNPs)
through
Joule
heating
impedance
spectroscopy.
platform
implemented
based
on
printed
circuit
board
(PCB)
technology,
has
many
advantages,
such
as
fast
easy
design
fabrication,
low
power
consumption,
costs.
process
energy
an
electric
current
converted
into
heat
it
flows
resistance.
Impedance
spectroscopy
analytical
technique
measures
system’s
electrical
response
applied
AC
signal
across
range
frequencies,
providing
insights
sample’s
dielectric
properties.
results
validate
fabricated
capable
20
µL
droplet
75
°C
within
30
s,
utilizing
input
only
3.75
watts
successfully
inducing
color
change
presence
hepatitis
C
virus
(HCV)
RNA,
while
readings
are
used
aggregation.
Язык: Английский
Prussian-Blue-Nanozyme-Enhanced Simultaneous Immunochromatographic Control of Two Relevant Bacterial Pathogens in Milk
Foods,
Год журнала:
2024,
Номер
13(19), С. 3032 - 3032
Опубликована: Сен. 24, 2024
Salmonella
typhimurium
and
Listeria
monocytogenes
are
relevant
foodborne
bacterial
pathogens
which
may
cause
serious
intoxications
infectious
diseases
in
humans.
In
this
study,
a
sensitive
immunochromatographic
analysis
(ICA)
for
the
simultaneous
detection
of
these
two
was
developed.
For
this,
test
strips
containing
zones
with
specific
monoclonal
antibodies
(MAb)
against
lipopolysaccharides
S.
L.
one
control
zone
secondary
were
designed,
double-assay
conditions
optimized
to
ensure
high
analytical
parameters.
Prussian
blue
nanoparticles
(PBNPs)
used
as
nanozyme
labels
conjugated
MAbs
perform
sandwich
format
ICA.
Peroxidase-mimic
properties
PBNPs
allowed
catalytic
amplification
colorimetric
signal
on
strips,
enhancing
assay
sensitivity.
The
limits
(LODs)
cells
2
×
102
7
103
cells/mL,
respectively.
LODs
100-fold
less
than
those
achieved
due
ICA
based
traditional
gold
label.
developed
double
approbated
bacteria
cow
milk
samples,
processed
by
simple
dilution
buffer
before
assay.
monocytogenes,
recoveries
from
86.3
±
9.8
118.2
10.5%
correlated
well
estimated
enzyme-linked
immunosorbent
reference
method.
proposed
approach
characterized
specificity:
no
cross-reactivity
other
strains
observed.
satisfies
requirements
rapid
tests:
full
cycle
sample
acquisition
result
assessment
half
an
hour.
has
application
potential
multiplex
pathogens.
Язык: Английский