Prussian-Blue-Nanozyme-Enhanced Simultaneous Immunochromatographic Control of Two Relevant Bacterial Pathogens in Milk DOI Creative Commons
Olga D. Hendrickson,

Nadezhda A. Byzova,

Boris B. Dzantiev

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(19), P. 3032 - 3032

Published: Sept. 24, 2024

Salmonella typhimurium and Listeria monocytogenes are relevant foodborne bacterial pathogens which may cause serious intoxications infectious diseases in humans. In this study, a sensitive immunochromatographic analysis (ICA) for the simultaneous detection of these two was developed. For this, test strips containing zones with specific monoclonal antibodies (MAb) against lipopolysaccharides S. L. one control zone secondary were designed, double-assay conditions optimized to ensure high analytical parameters. Prussian blue nanoparticles (PBNPs) used as nanozyme labels conjugated MAbs perform sandwich format ICA. Peroxidase-mimic properties PBNPs allowed catalytic amplification colorimetric signal on strips, enhancing assay sensitivity. The limits (LODs) cells 2 × 102 7 103 cells/mL, respectively. LODs 100-fold less than those achieved due ICA based traditional gold label. developed double approbated bacteria cow milk samples, processed by simple dilution buffer before assay. monocytogenes, recoveries from 86.3 ± 9.8 118.2 10.5% correlated well estimated enzyme-linked immunosorbent reference method. proposed approach characterized specificity: no cross-reactivity other strains observed. satisfies requirements rapid tests: full cycle sample acquisition result assessment half an hour. has application potential multiplex pathogens.

Language: Английский

Application and development of biosensing strategies for the analysis of the activity of the tumour-associated enzyme FEN1 DOI
Siyi Chen,

Feiran Gao,

Lin Zhao

et al.

Talanta, Journal Year: 2025, Volume and Issue: 286, P. 127527 - 127527

Published: Jan. 7, 2025

Language: Английский

Citations

0
Rapid Detection of microRNA-122 in Serum and Finger Blood Using a Lateral Flow Nucleic Acid Biosensor DOI Creative Commons
Min Zhang,

Meijing Ma,

Jiahui Wang

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 58 - 58

Published: Jan. 17, 2025

MicroRNA122 (miR-122) is a microRNA that highly expressed in hepatocytes and has been identified as prospective therapeutic target biomarker for liver injury. An expanding body of research demonstrated miR-122 critical regulator both the initiation progression wide range diseases. Traditional methods detecting mainly include Northern blotting qRT-PCR, but they are technically complex cumbersome, requiring expensive instruments high technical requirements. In this paper, we present novel rapid testing method utilizing lateral flow nucleic acid biosensor (LFNAB) sensitive time-efficient detection miR-122. This approach offers several advantages, including specificity miR-122, ability to detect low concentrations molecule, significantly reduced time compared conventional methods. study, thiol-modified single-stranded DNA probe (Det-DNA), biotinylated capture (Cap-DNA), control (Con-DNA) used construct LFNAB. A gold nanoparticle (AuNP) colored tag, which label Det-DNA probe. The principle based on dual DNA-miRNA hybridization reactions LFNAB form sandwich-type AuNP-Det-DNA-miR-122-Cap-DNA complexes, captured test area visualization quantification. After systematic optimization conditions experiment, response was linear within scope 0 pM-100 pM limit 15 min 3.90 pM. use serum fingertip blood yielded satisfactory results. successful application indicates effectiveness samples, showcasing its potential utility clinical settings assessing levels different biological samples.

Language: Английский

Citations

0
Bioconvergence: advancing biosensors with nanotechnology for real-time health monitoring DOI Creative Commons
Rosaline Mishra,

Sakshi Minocha,

Radha Goel

et al.

Bulletin of the National Research Centre/Bulletin of the National Research Center, Journal Year: 2025, Volume and Issue: 49(1)

Published: Feb. 14, 2025

Abstract Background Biosensors are tools that products of amalgamation biological constituents and physicochemical elements so as to detect analytes, based on signal production which is measurable. Thus here we can see an assimilation biology microelectronics. Keeping a track biochemical processes in human system essential; thus, there increasing need for extremely sensitive economical devices make precise diagnosis personalised medication treatment possible. Objective This review focuses upon the novel innovations field biosensors, namely making use nanotechnology form nanomaterials such graphene, gold nanoparticles, quantum dots, sulphur-containing nanomaterials. Method A thorough evaluation literature was done. Databases like SciFinder, ScienceDirect, PubMed, Google, Google Scholar, Egyptian Knowledge Bank were used find pertinent publications. More than 500 articles databases studied constitute this paper. The accuracy retrieved data carefully reviewed cross-checked. Selection nanomaterial creation biosensors studied: Nanomaterials (such carbon nanotubes, dots) according their special qualities high surface area, biocompatibility, sensitivity) selected. study biomolecular integration done: To gain knowledge selective target integrate particular biomolecules enzymes, aptamers, antibodies) onto also manufacturing sensors utilised; sophisticated methods self-assembly lithography) build have exact control over arrangement studied. Signal Transduction: transform interactions into quantifiable signals, effectively develop transduction systems (e.g. electrochemical, optical, mechanical). Real-time Monitoring: For ongoing, non-invasive health monitoring, include with wearable technology or point-of-care diagnostics. Data analysis: interpret analyse biosensor signals rapid evaluations, apply machine learning techniques analysis algorithms. Result After applying article selection criteria reviewing quality methodology, total 200 selected be included review. Conclusion combination nanotechnology, "bioconvergence", has enormous potential healthcare. real-time, monitoring built by utilising distinct specificity biomolecules. could lead more efficient individualised healthcare improving disease diagnosis, treatment, overall patient outcomes.

Language: Английский

Citations

0
Epigenetic insights of microRNAs: Pioneering cancer detection via advanced biosensors DOI

Parveen Usman,

Durairaj Sekar

International review of cell and molecular biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Microalgae in health care and functional foods: β-glucan applications, innovations in drug delivery and synthetic biology DOI Creative Commons
Chao Li, Ming‐Qing Du,

Yujie Han

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 4, 2025

Microalgae are emerging as a key player in healthcare, functional foods, and sustainable biotech due to their capacity produce bioactive compounds like β-glucans, omega-3 fatty acids, antioxidants an eco-friendly manner. This review comprehensively discusses the role of microalgae healthcare focusing particularly on β-glucan therapeutics, drug delivery innovations, synthetic biology applications. In microalgae-derived show immense promise for treating diseases, boosting immunity, tackling oxidative stress. Euglena -derived paramylon, type β-glucan, has shown potential various medical applications, including immunomodulation anticancer therapy. Synthetic bioprocess engineering enhancing microalgae’s therapeutic nutritional value, with applications personalized medicine. To maximize microalgae, further research development needed address scalability, regulatory alignment, consumer acceptance, focus interdisciplinary collaboration practices align innovation environmental conservation.

Language: Английский

Citations

0
Revolutionary advances in hypertension detection: Gold nanoparticle-enhanced miRNA-based electrochemical biosensors and emerging nanotechnologies DOI

Parveen Usman,

K P Ameya,

Durairaj Sekar

et al.

Human Gene, Journal Year: 2025, Volume and Issue: unknown, P. 201401 - 201401

Published: March 1, 2025

Language: Английский

Citations

0
The Potential of Optical Technologies in Early Virus Detection; Prospects in Addressing Future Viral Outbreaks DOI
Mehdi Kheirollahpour, Nader Shokoufi, Mohsen Lotfi

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 29

Published: March 27, 2025

The urgent need for sensitive, rapid, and reliable diagnostic methodologies to control prevent life-threatening pandemic infectious disease, such as COVID-19, remains a critical priority. Timely on-site detection of viral pathogens is essential effective disease management mitigation societal disruptions. Recent advancements in optical methods have positioned them at the forefront healthcare diagnostics, offering high sensitivity specificity viable alternatives conventional techniques Polymerase Chain Reaction (PCR), which often suffer from time delays limited accessibility resource-constrained environments. This review elucidates potential various techniques, highlighting their advantages over traditional methods. It encompasses range modalities, including fluorescence-based approaches, Raman spectroscopy (RS), Plasmonic (e.g., surface plasmon resonance (SPR), localized SPR, (LSPR), surface-enhanced (SERS), fluorescence (SEF)), super resolution microscopies (SRMs), attenuated total reflectance-Fourier transform infrared (ATR-FTIR), integrated platforms waveguides molecularly imprinted polymer (MIP)-based biosensors. Additionally, evolution novel biosensors, particularly 5th 6th generation challenges related these technologies were discussed. studies reviewed aims advance development portable, specific, cost-effective point-of-care (POC) devices rapid pathogens.

Language: Английский

Citations

0
Advancing gut microbiome insights: Graphene quantum dot nanobiosensors for microbial metabolite detection DOI
Soheil Sadr,

Shakiba Nazemian,

Shiva Dianaty

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162954 - 162954

Published: April 1, 2025

Citations

0
Prussian-Blue-Nanozyme-Enhanced Simultaneous Immunochromatographic Control of Two Relevant Bacterial Pathogens in Milk DOI Creative Commons
Olga D. Hendrickson,

Nadezhda A. Byzova,

Boris B. Dzantiev

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(19), P. 3032 - 3032

Published: Sept. 24, 2024

Salmonella typhimurium and Listeria monocytogenes are relevant foodborne bacterial pathogens which may cause serious intoxications infectious diseases in humans. In this study, a sensitive immunochromatographic analysis (ICA) for the simultaneous detection of these two was developed. For this, test strips containing zones with specific monoclonal antibodies (MAb) against lipopolysaccharides S. L. one control zone secondary were designed, double-assay conditions optimized to ensure high analytical parameters. Prussian blue nanoparticles (PBNPs) used as nanozyme labels conjugated MAbs perform sandwich format ICA. Peroxidase-mimic properties PBNPs allowed catalytic amplification colorimetric signal on strips, enhancing assay sensitivity. The limits (LODs) cells 2 × 102 7 103 cells/mL, respectively. LODs 100-fold less than those achieved due ICA based traditional gold label. developed double approbated bacteria cow milk samples, processed by simple dilution buffer before assay. monocytogenes, recoveries from 86.3 ± 9.8 118.2 10.5% correlated well estimated enzyme-linked immunosorbent reference method. proposed approach characterized specificity: no cross-reactivity other strains observed. satisfies requirements rapid tests: full cycle sample acquisition result assessment half an hour. has application potential multiplex pathogens.

Language: Английский

Citations

1