Scientific Reports,
Год журнала:
2020,
Номер
10(1)
Опубликована: Июнь 26, 2020
Abstract
Trisomy
21
(Down
syndrome,
DS)
is
the
main
human
genetic
cause
of
intellectual
disability
(ID).
Lejeune
hypothesized
that
DS
could
be
considered
a
metabolic
disease,
and
we
found
subjects
with
have
specific
plasma
urinary
metabolomic
profile.
In
this
work
confirmed
alteration
mitochondrial
metabolism
in
also
investigated
if
metabolite
levels
are
related
to
cognitive
aspects
DS.
We
analyzed
profiles
samples
from
129
46
healthy
control
(CTRL)
by
1
H
Nuclear
Magnetic
Resonance
(NMR).
Multivariate
analysis
NMR
showed
clear
discrimination
(up
94%
accuracy)
between
two
groups.
The
univariate
revealed
significant
7
metabolites
out
28
assigned
unambiguously.
Correlations
among
CTRL
groups
were
separately
statistically
relationships
appeared.
On
contrary,
correlations
NMR-detectable
part
metabolome
different
intelligence
quotient
ranges
obtained
Griffiths-III
or
WPPSI-III
tests
not
found.
Even
imbalance
provides
groups,
it
appears
cannot
associated
degree
ID.
Metabolites,
Год журнала:
2019,
Номер
9(7), С. 123 - 123
Опубликована: Июнь 27, 2019
Over
the
past
two
decades,
nuclear
magnetic
resonance
(NMR)
has
emerged
as
one
of
three
principal
analytical
techniques
used
in
metabolomics
(the
other
being
gas
chromatography
coupled
to
mass
spectrometry
(GC-MS)
and
liquid
with
single-stage
(LC-MS)).
The
relative
ease
sample
preparation,
ability
quantify
metabolite
levels,
high
level
experimental
reproducibility,
inherently
nondestructive
nature
NMR
spectroscopy
have
made
it
preferred
platform
for
long-term
or
large-scale
clinical
metabolomic
studies.
These
advantages,
however,
are
often
outweighed
by
fact
that
most
techniques,
including
both
LC-MS
GC-MS,
more
sensitive
than
NMR,
lower
limits
detection
typically
10
100
times
better.
This
review
is
intended
introduce
readers
field
NMR-based
highlight
advantages
disadvantages
It
will
also
explore
some
unique
strengths
metabolomics,
particularly
regard
isotope
selection/detection,
mixture
deconvolution
via
2D
spectroscopy,
automation,
noninvasively
analyze
native
tissue
specimens.
Finally,
this
a
number
emerging
technologies
strengthen
its
utility
overcome
inherent
limitations
applications.
Angewandte Chemie International Edition,
Год журнала:
2018,
Номер
58(4), С. 968 - 994
Опубликована: Июль 12, 2018
Metabolomics
deals
with
the
whole
ensemble
of
metabolites
(the
metabolome).
As
one
-omic
sciences,
it
relates
to
biology,
physiology,
pathology
and
medicine;
but
are
chemical
entities,
small
organic
molecules
or
inorganic
ions.
Therefore,
their
proper
identification
quantitation
in
complex
biological
matrices
requires
a
solid
ground.
With
respect
for
example,
DNA,
much
more
prone
oxidation
enzymatic
degradation:
we
can
reconstruct
large
parts
mammoth's
genome
from
specimen,
unable
do
same
its
metabolome,
which
was
probably
largely
degraded
few
hours
after
animal's
death.
Thus,
need
standard
operating
procedures,
good
skills
sample
preparation
storage
subsequent
analysis,
accurate
analytical
broad
knowledge
chemometrics
advanced
statistical
tools,
at
least
two
metabolomic
techniques,
MS
NMR.
All
these
traditionally
cultivated
by
chemists.
Here
focus
on
metabolomics
standpoint
restrict
ourselves
From
point
view,
NMR
has
pros
cons
does
provide
peculiar
holistic
perspective
that
may
speak
future
adoption
as
population-wide
health
screening
technique.
Molecules,
Год журнала:
2020,
Номер
25(20), С. 4597 - 4597
Опубликована: Окт. 9, 2020
Studying
disease
models
at
the
molecular
level
is
vital
for
drug
development
in
order
to
improve
treatment
and
prevent
a
wide
range
of
human
pathologies.
Microbial
infections
are
still
major
challenge
because
pathogens
rapidly
continually
evolve
developing
resistance.
Cancer
cells
also
change
genetically,
current
therapeutic
techniques
may
be
(or
become)
ineffective
many
cases.
The
pathology
neurological
diseases
remains
an
enigma,
exact
etiology
underlying
mechanisms
largely
unknown.
Viral
spread
develop
much
more
quickly
than
does
corresponding
research
needed
combat
these
infections;
present
most
relevant
outbreak
SARS-CoV-2,
which
originated
Wuhan,
China,
illustrates
critical
immediate
need
design
techniques.
Modern
day
discovery
time-consuming,
expensive
process.
Each
new
takes
excess
10
years
costs
on
average
billion
US
dollars.
This
demonstrates
complete
redesign
or
novel
strategies.
Nuclear
Magnetic
Resonance
(NMR)
has
played
role
ever
since
its
introduction
several
decades
ago.
In
just
three
decades,
NMR
become
“gold
standard”
platform
technology
medical
pharmacology
studies.
this
review,
we
applications
spectroscopy
development.
basic
concepts,
theories,
commonly
used
presented.
We
summarize
advantages
limitations
primary
methods
Metabolites,
Год журнала:
2021,
Номер
11(3), С. 151 - 151
Опубликована: Март 7, 2021
Dynamic
changes
in
circulating
and
tissue
metabolites
lipids
occur
response
to
exercise-induced
cellular
whole-body
energy
demands
maintain
metabolic
homeostasis.
The
metabolome
lipidome
a
given
biological
system
provides
molecular
snapshot
of
these
rapid
complex
perturbations.
application
metabolomics
lipidomics
map
the
responses
an
acute
bout
aerobic/endurance
or
resistance
exercise
has
dramatically
expanded
over
past
decade
thanks
major
analytical
advancements,
with
most
exercise-related
studies
date
focused
on
analyzing
human
biofluids
tissues.
Experimental
considerations,
as
well
complementary
using
animal
model
systems,
are
warranted
help
overcome
challenges
associated
large
interindividual
variability
decipher
breadth
mechanisms
underlying
health-promoting
effects
exercise.
In
this
review,
we
provide
guide
for
researchers
regarding
techniques
experimental
workflows
commonly
used
lipidomics.
Furthermore,
discuss
advancements
mammalian
research
utilizing
metabolomic
lipidomic
approaches
last
decade,
highlight
key
technical
considerations
remaining
knowledge
gaps
continue
expanding
landscape
biology.
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2021,
Номер
20(2), С. 2040 - 2062
Опубликована: Янв. 27, 2021
Abstract
Wine
authentication
is
vital
in
identifying
malpractice
and
fraud,
various
physical
chemical
analytical
techniques
have
been
employed
for
this
purpose.
Besides
wet
chemistry,
these
include
chromatography,
isotopic
ratio
mass
spectrometry,
optical
spectroscopy,
nuclear
magnetic
resonance
(NMR)
which
applied
recent
years
combination
with
chemometric
approaches.
For
many
years,
2
H
NMR
spectroscopy
was
the
method
of
choice
achieved
official
recognition
detection
sugar
addition
to
grape
products.
Recently,
1
a
simpler
faster
(in
terms
sample
preparation),
has
gathered
more
attention
wine
analysis,
even
if
it
still
lacks
recognition.
This
technique
makes
targeted
quantitative
determination
ingredients
nontargeted
metabolomic
fingerprint
possible.
review
summarizes
possibilities
limitations
authentication,
by
reviewing
its
applications
as
reported
literature.
Examples
commercial
open‐source
solutions
combining
chemometrics
are
also
examined
herein,
together
opportunities
becoming
an
method.
Briefings in Bioinformatics,
Год журнала:
2021,
Номер
22(5)
Опубликована: Апрель 16, 2021
Individual
variations
in
drug
efficacy,
side
effects
and
adverse
reactions
are
still
challenging
that
cannot
be
ignored
research
development.
The
aim
of
pharmacometabonomics
is
to
better
understand
the
pharmacokinetic
properties
drugs
monitor
on
specific
metabolic
pathways.
Here,
we
systematically
reviewed
recent
technological
advances
for
understanding
pathophysiological
mechanisms
diseases
as
well
bodies.
First,
advantages
disadvantages
all
mainstream
analytical
techniques
were
compared.
Second,
many
data
processing
strategies
including
filtering,
missing
value
imputation,
quality
control-based
correction,
transformation,
normalization
together
with
methods
implemented
each
step
discussed.
Third,
various
feature
selection
extraction
algorithms
commonly
applied
described.
Finally,
databases
facilitate
current
collected
All
all,
this
review
provided
guidance
researchers
engaged
metabolomics,
it
would
promote
wide
application
metabolomics
personalized
medicine.
Frontiers in Pharmacology,
Год журнала:
2022,
Номер
13
Опубликована: Март 21, 2022
Fluxomics
is
an
innovative
-omics
research
field
that
measures
the
rates
of
all
intracellular
fluxes
in
central
metabolism
biological
systems.
gathers
data
from
multiple
different
fields,
portraying
whole
picture
molecular
interactions.
Recently,
fluxomics
has
become
one
most
relevant
approaches
to
investigate
metabolic
phenotypes.
Metabolic
flux
using
Annual Review of Food Science and Technology,
Год журнала:
2019,
Номер
10(1), С. 479 - 519
Опубликована: Март 25, 2019
Metabolomics
is
the
study
of
small
molecules
called
metabolites
in
biological
samples.
Application
metabolomics
to
nutrition
research
has
expanded
recent
years,
with
emerging
literature
supporting
multiple
applications.
Key
examples
include
applications
identification
and
development
objective
biomarkers
dietary
intake,
developing
personalized
strategies,
large-scale
epidemiology
studies
understand
link
between
diet
health.
In
this
review,
we
provide
an
overview
current
identify
key
challenges
that
need
be
addressed
for
further
field.
Successful
potential
improve
assessment,
help
deliver
nutrition,
enhance
our
understanding
RSC Advances,
Год журнала:
2021,
Номер
11(15), С. 8694 - 8700
Опубликована: Янв. 1, 2021
NMR-based
metabolomics,
which
emerged
along
with
mass
spectrometry
techniques,
is
the
preferred
method
for
studying
metabolites
in
medical
research
and
food
industries.
However,
NMR
techniques
suffer
from
inherently
low
sensitivity,
regardless
of
their
superior
reproducibility.
To
overcome
this,
we
made
two
beneficial
modifications:
detuned
probe
to
reach
a
position
called
"Spin
Noise
Tuning
Optimum"
(SNTO),
replaced
conventional
cylindrical
5
mm
tube
an
electric
field
component-optimized
shaped
tube.
We
found
that
concerted
use
both
modifications
can
increase
sensitivity
(signal
noise
ratio
per
unit
volume)
detection
decrease
measurement
time
by
order
magnitude.
In
this
study,
demonstrate
discuss
achieved
signal
enhancement
on
model
non-human
(bovine
serum,
amino
acid
standard
mixture)
human
urine
samples.