Aging, Год журнала: 2023, Номер unknown
Опубликована: Авг. 11, 2023
Язык: Английский
Mucosal Immunology, Год журнала: 2022, Номер 15(6), С. 1143 - 1157
Опубликована: Авг. 24, 2022
Язык: Английский
Процитировано
58
The Ocular Surface, Год журнала: 2022, Номер 25, С. 108 - 118
Опубликована: Июнь 23, 2022
Язык: Английский
Процитировано
52
Annual Review of Immunology, Год журнала: 2023, Номер 41(1), С. 181 - 205
Опубликована: Апрель 26, 2023
There is a dramatic remodeling of the T cell compartment during aging. The most notorious changes are reduction naive pool and accumulation memory-like cells. Memory-like cells in older people acquire phenotype terminally differentiated cells, lose expression costimulatory molecules, properties senescent In this review, we focus on different subsets age-associated that accumulate These include extremely cytotoxic with natural killer properties, exhausted altered cytokine production, regulatory gain proinflammatory features. Importantly, all these their lymph node homing capacity migrate preferentially to nonlymphoid tissues, where they contribute tissue deterioration inflammaging.
Язык: Английский
Процитировано
39
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(2), С. 1208 - 1208
Опубликована: Янв. 19, 2024
The lacrimal gland is responsible for maintaining the health of ocular surface through production tears. However, our understanding immune system within currently limited. Therefore, in this study, we utilized single-cell RNA sequencing and bioinformatic analysis to identify analyze cells molecules present glands normal mice. A total 34,891 were obtained from mice classified into 18 distinct cell clusters using Seurat clustering. Within these populations, 26 different subpopulations identified, including T cells, innate lymphocytes, macrophages, mast dendritic B cells. Network revealed complex cell-cell interactions between with particularly significant observed among plasma Interestingly, found be main source ligands Thy1 signaling pathway, while M2 macrophages identified as primary target pathway. Moreover, some validated immunohistological techniques. Collectively, findings highlight abundance provide valuable insights complexity its relevance associated diseases.
Язык: Английский
Процитировано
6
Clinical Immunology, Год журнала: 2023, Номер 248, С. 109251 - 109251
Опубликована: Фев. 3, 2023
Aging is a complex biological process in which many organs are pathologically affected. We previously reported that aged C57BL/6J had increased lacrimal gland (LG) lymphoid infiltrates suggest ectopic structures. However, these structures have not been fully investigated. Using mice of different ages, we analyzed the transcriptome murine LGs and characterized B T cell populations. Age-related changes LG include differentially expressed genes associated with activation, germinal center formation, infiltration by marginal zone-like cells. also identified an age-related increase B1
Язык: Английский
Процитировано
13
Aging Cell, Год журнала: 2024, Номер 23(10)
Опубликована: Авг. 18, 2024
CD4
Язык: Английский
Процитировано
5
GeroScience, Год журнала: 2025, Номер unknown
Опубликована: Март 7, 2025
Язык: Английский
Процитировано
0
Investigative Ophthalmology & Visual Science, Год журнала: 2023, Номер 64(12), С. 18 - 18
Опубликована: Сен. 11, 2023
This study used high-throughput RNA sequencing (RNA-Seq) and bioinformatics analysis to investigate the altered transcriptome profile of aging lacrimal glands in mice that occurs over course a 24-hour cycle.Male C57BL/6J aged 12 weeks (young) 20 months (aging) were housed pathogen-free setting with 12-hour light/12-hour dark cycle. Throughout cycle, mouse extraorbital (ELGs) collected at eight time points three-hour intervals. To prepare for RNA-Seq, whole mRNA was extracted. Differentially expressed genes (DEGs) young groups subjected bioinformatic based on diurnal patterns. Furthermore, cell populations which significant DEGs express signaling pathways occur validated single-cell (scRNA-seq) level.The total composition significantly ELGs compared during 864 upregulated 228 downregulated DEGs, primarily enriched inflammatory pathways. Further comparative point-to-point revealed underwent alterations temporal several pathways, including inflammation-related, metabolism-related, mitochondrial bioenergetic function-associated, synaptome neural activity-associated, processes-associated, DNA processing-associated fibrosis-associated Most these occurred separately distinct populations.Transcriptome profiles undergo considerable time-dependent changes; this finding offers comprehensive source information better understand pathophysiology gland its underlying mechanisms.
Язык: Английский
Процитировано
10
Croatian Medical Journal, Год журнала: 2023, Номер 64(5), С. 307 - 319
Опубликована: Окт. 1, 2023
To assess sex-related differences in the pathohistological features of human lacrimal gland and to investigate age-related stereologically measured volume density secretory tissue, connective fat.We performed an observational analysis acinar atrophy, periacinar fibrosis, periductal ductal dilation, proliferation, fatty infiltration, lymphocyte infiltration hematoxylin eosin-stained samples from 81 cornea donors. Stereological fat was on 66 donors.Up 69% all showed degenerative changes. Female had a higher frequency observed changes, except dilation. While atrophy significantly more prevalent women, dilation men. indicated lower portions acini tissue fat, as well pronounced progression changes female samples.Female glands are susceptible degeneration, this susceptibility could play important role incidence dry eye disease older women. A further stereological using younger age groups is needed elucidate structure their impact disease.
Язык: Английский
Процитировано
7
Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(5), С. 21 - 21
Опубликована: Май 13, 2024
Aging is a risk factor for dry eye. We sought to identify changes in the aged mouse corneal epithelial transcriptome and determine how age affects sensitivity, re-epithelialization, barrier reformation after debridement.
Язык: Английский
Процитировано
2