Journal of genetics and genomics/Journal of Genetics and Genomics,
Год журнала:
2023,
Номер
50(11), С. 846 - 860
Опубликована: Авг. 21, 2023
Bread
wheat
provides
an
essential
fraction
of
the
daily
calorific
intake
for
humanity.
Due
to
its
huge
and
complex
genome,
progresses
in
studying
on
genome
are
substantially
trailed
behind
those
other
two
major
crops,
rice
maize,
at
least
a
decade.
With
rapid
advances
assembling
reduced
cost
high-throughput
sequencing,
emerging
de
novo
assemblies
whole-genome
sequencing
data
leading
paradigm
shift
research.
Here,
we
review
recent
progress
dissecting
germplasm
evolution
since
release
first
high-quality
genome.
New
insights
have
been
gained
during
domestication
modern
breeding
progress,
genomic
variations
multiple
scales
contributing
diversity
germplasm,
transcriptional
epigenetic
regulations
functional
genes
polyploid
wheat.
Genomics
databases
bioinformatics
tools
meeting
urgent
needs
genomics
research
also
summarized.
The
ever-increasing
omics
data,
along
with
advanced
well-structured
databases,
expected
accelerate
deciphering
gene
resources
future
advances.
Agriculture,
Год журнала:
2023,
Номер
13(3), С. 642 - 642
Опубликована: Март 8, 2023
As
one
of
the
essential
cereal
crops,
wheat
provides
20%
calories
and
proteins
consumed
by
humans.
Due
to
population
expansion,
dietary
shift
climate
change,
it
is
challenging
for
breeders
develop
new
varieties
meeting
production
requirements.
Marker-assisted
selection
(MAS)
has
distinct
advantages
over
conventional
in
plant
breeding,
such
as
being
time-saving,
cost-effective
goal-oriented.
This
review
makes
attempts
give
a
description
different
molecular
markers:
sequence
tagged
site
(STS),
simple
repeat
(SSR),
genotyping
sequencing
(GBS),
single
nucleotide
polymorphism
(SNP)
arrays,
exome
capture,
Kompetitive
Allele
Specific
PCR
(KASP),
cleaved
amplified
polymorphic
(CAPS),
semi-thermal
asymmetric
reverse
(STARP)
target
(GBTS).
We
also
summarize
some
quantitative
trait
loci
(QTL)/genes
well
their
linked
markers,
which
are
potentially
useful
MAS.
paper
updated
information
on
markers
critical
traits
potential
applications
breeding
programs.
Prime
editing
is
limited
by
low
efficiency
in
plants.
Here,
we
develop
an
upgraded
engineered
plant
prime
editor
hexaploid
wheat,
ePPEplus,
introducing
a
V223A
substitution
into
reverse
transcriptase
the
ePPEmax*
architecture.
ePPEplus
enhances
average
33.0-fold
and
6.4-fold
compared
to
original
PPE
ePPE,
respectively.
Importantly,
robust
multiplex
platform
established
for
simultaneous
of
four
ten
genes
protoplasts
up
eight
regenerated
wheat
plants
at
frequencies
74.5%,
thus
expanding
applicability
editors
stacking
multiple
agronomic
traits.
Plant Communications,
Год журнала:
2023,
Номер
4(4), С. 100593 - 100593
Опубликована: Март 21, 2023
A
better
understanding
of
wheat
functional
genomics
can
improve
targeted
breeding
for
agronomic
traits
and
environmental
adaptation.
However,
the
lack
gene-indexed
mutants
low
transformation
efficiency
limit
in-depth
gene
studies
genetic
manipulation
breeding.
In
this
study,
we
created
a
library
KN9204,
popular
variety
in
northern
China,
with
reference
genome,
transcriptome,
epigenome
different
tissues,
using
ethyl
methyl
sulfonate
(EMS)
mutagenesis.
This
contains
vast
developmental
diversity
critical
tissues
transition
stages.
Exome
capture
sequencing
2090
mutant
lines
KN9204
genome-designed
probes
revealed
that
98.79%
coding
genes
had
mutations,
each
line
an
average
1383
EMS-type
SNPs.
We
identified
new
allelic
variations
crucial
trait-related
such
as
Rht-D1,
Q,
TaTB1,
WFZP.
tested
100
severe
mutations
80
NAC
transcription
factors
(TFs)
under
drought
salinity
stress
13
altered
sensitivity.
Further
analysis
three
transcriptome
chromatin
accessibility
data
hundreds
direct
targets
patterns
salt
or
stress,
including
SNAC1,
DREB2B,
CML16,
ZFP182,
known
to
respond
abiotic
stress.
Thus,
have
generated
indexed
EMS
facilitate
research
offer
resources
wheat.
