aBIOTECH,
Journal Year:
2024,
Volume and Issue:
5(1), P. 52 - 70
Published: Feb. 7, 2024
Abstract
Bread
wheat
(
Triticum
aestivum
)
is
an
important
crop
and
serves
as
a
significant
source
of
protein
calories
for
humans,
worldwide.
Nevertheless,
its
large
allopolyploid
genome
poses
constraints
on
genetic
improvement.
The
complex
reticulate
evolutionary
history
the
intricacy
genomic
resources
make
deciphering
functional
considerably
more
challenging.
Recently,
we
have
developed
comprehensive
list
versatile
computational
tools
with
integration
statistical
models
dissecting
polyploid
genome.
Here,
summarize
methodological
innovations
applications
these
databases.
A
series
step-by-step
examples
illustrates
how
can
be
utilized
germplasm
unveiling
genes
associated
agronomic
traits.
Furthermore,
outline
future
perspectives
new
advanced
databases,
taking
into
consideration
unique
features
bread
wheat,
to
accelerate
genomic-assisted
breeding.
Agriculture,
Journal Year:
2023,
Volume and Issue:
13(3), P. 642 - 642
Published: March 8, 2023
As
one
of
the
essential
cereal
crops,
wheat
provides
20%
calories
and
proteins
consumed
by
humans.
Due
to
population
expansion,
dietary
shift
climate
change,
it
is
challenging
for
breeders
develop
new
varieties
meeting
production
requirements.
Marker-assisted
selection
(MAS)
has
distinct
advantages
over
conventional
in
plant
breeding,
such
as
being
time-saving,
cost-effective
goal-oriented.
This
review
makes
attempts
give
a
description
different
molecular
markers:
sequence
tagged
site
(STS),
simple
repeat
(SSR),
genotyping
sequencing
(GBS),
single
nucleotide
polymorphism
(SNP)
arrays,
exome
capture,
Kompetitive
Allele
Specific
PCR
(KASP),
cleaved
amplified
polymorphic
(CAPS),
semi-thermal
asymmetric
reverse
(STARP)
target
(GBTS).
We
also
summarize
some
quantitative
trait
loci
(QTL)/genes
well
their
linked
markers,
which
are
potentially
useful
MAS.
paper
updated
information
on
markers
critical
traits
potential
applications
breeding
programs.
Genome biology,
Journal Year:
2023,
Volume and Issue:
24(1)
Published: June 29, 2023
Prime
editing
is
limited
by
low
efficiency
in
plants.
Here,
we
develop
an
upgraded
engineered
plant
prime
editor
hexaploid
wheat,
ePPEplus,
introducing
a
V223A
substitution
into
reverse
transcriptase
the
ePPEmax*
architecture.
ePPEplus
enhances
average
33.0-fold
and
6.4-fold
compared
to
original
PPE
ePPE,
respectively.
Importantly,
robust
multiplex
platform
established
for
simultaneous
of
four
ten
genes
protoplasts
up
eight
regenerated
wheat
plants
at
frequencies
74.5%,
thus
expanding
applicability
editors
stacking
multiple
agronomic
traits.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Jan. 13, 2024
Abstract
Powdery
mildew
poses
a
significant
threat
to
wheat
crops
worldwide,
emphasizing
the
need
for
durable
disease
control
strategies.
The
wheat-
Dasypyrum
villosum
T5AL·5
V#4
S
and
T5DL·5
translocation
lines
carrying
powdery
resistant
gene
Pm55
shows
developmental-stage
tissue-specific
resistance,
whereas
V#5
line
Pm5V
confers
resistance
at
all
stages.
Here,
we
clone
,
reveal
that
they
are
allelic
renamed
as
Pm55a
Pm55b
respectively.
two
alleles
encode
coiled-coil,
nucleotide-binding
site-leucine-rich
repeat
(CNL)
proteins,
conferring
broad-spectrum
mildew.
However,
interact
differently
with
linked
inhibitor
gene,
SuPm55
cause
different
Notably,
unrelated
CNL
inactivation
of
significantly
reduces
plant
fitness.
Combining
/
in
does
not
result
allele
suppression
or
yield
penalty.
Our
results
provide
only
insights
into
wheat,
but
also
strategy
breeding
resistance.
