Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Окт. 23, 2024
Herpesviruses
are
a
family
of
double-stranded
DNA
viruses
with
tegument
structure
and
genome
composed
single
sequence
terminal
repeat
(TR)
sequences.
The
herpesvirus
UL14
gene
encodes
the
protein
(pUL14),
which
has
various
subcellular
localizations
plays
vital
role
in
regulating
immediate-early
(IE)
transcription
expression,
influences
intracellular
localization
patterns
several
proteins
belonging
to
capsid
packaging
machinery,
participates
secondary
envelopment,
viral
particle
release.
Additionally,
pUL14
roles
maintaining
cellular
homeostasis
preventing
apoptosis.
This
review
discusses
how
engages
life
cycle
herpesviruses
provides
new
ideas
for
further
research
on
pUL14's
function
infection.
Viruses,
Год журнала:
2023,
Номер
15(7), С. 1434 - 1434
Опубликована: Июнь 25, 2023
As
one
of
the
most
important
avian
immunosuppressive
and
neoplastic
diseases,
Marek's
disease
(MD),
caused
by
oncogenic
virus
(MDV),
has
huge
economic
losses
worldwide
over
past
five
decades.
In
recent
years,
MD
outbreaks
have
occurred
frequently
in
MD-vaccinated
chicken
flocks,
but
key
pathogenic
determinants
influencing
factors
remain
unclear.
Herein,
we
analyzed
pathogenicity
seven
newly
isolated
MDV
strains
from
tumor-bearing
chickens
China
found
that
all
them
were
to
hosts,
among
which
four
isolates,
SDCW01,
HNXZ05,
HNSQ05
HNSQ01,
considered
be
hypervirulent
(HV-MDV)
strains.
At
73
days
infection
experiment,
cumulative
incidences
100%,
93.3%,
90%
with
mortalities
83.3%,
73.3%,
60%
86.7%,
respectively,
for
viruses.
The
gross
occurrences
tumors
50%,
33.3%,
30%
63.3%,
accompanied
significant
hepatosplenomegaly
serious
atrophy
immune
organs.
Furthermore,
protection
effects
commercial
vaccines
against
CVI988,
HVT,
CVI988+HVT,
814
explored.
Unexpectedly,
during
67
post-virus
challenge,
indices
(PIs)
these
only
46.2%,
38.5%,
28%,
birds
received
monovalent
CVI988
or
HVT
still
developed
7.7%
11.5%,
respectively.
To
our
knowledge,
this
is
first
demonstration
simultaneous
comparison
efficacy
multiple
different
vaccine
Our
study
revealed
HV-MDV
variants
circulating
could
significantly
break
through
classical
currently
widely
used.
For
future
work,
there
an
urgent
need
develop
novel,
more
effective
tackling
new
challenge
emerging
sustainable
control
MD.
Marek's
disease
virus
(MDV)
is
a
highly
pathogenic
and
oncogenic
alpha
herpesvirus
that
causes
(MD),
which
one
of
the
most
important
immunosuppressive
rapid-onset
neoplastic
diseases
in
poultry.
The
onset
MD
lymphomas
other
clinical
can
be
efficiently
prevented
by
vaccination;
these
vaccines
are
heralded
as
first
demonstration
successful
vaccination
strategy
against
cancer.
However,
persistent
evolution
epidemic
MDV
strains
towards
greater
virulence
has
recently
resulted
frequent
outbreaks
vaccinated
chicken
flocks
worldwide.
Herein,
we
provide
an
overall
review
focusing
on
discovery
identification
strategies
evades
host
immunity
attacks
immune
system.
We
have
also
highlighted
decrease
efficacy
current
vaccines.
prospects,
new
techniques
for
development
efficient
vaccines,
together
with
possibilities
antiviral
therapy
MD,
discussed.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2025,
Номер
15
Опубликована: Март 13, 2025
Circular
RNAs
(circRNAs)
are
a
class
of
non-coding
with
covalently
closed
circular
structure,
lacking
5’-caps
or
3’-poly(A)
tails.
They
relatively
conserved,
highly
stable,
and
often
exhibit
tissue-
cell-specific
production
in
eukaryotic
cells.
Based
on
the
advances
sequencing
technologies
bioinformatics,
multiple
reports
have
suggested
that
viruses
other
microorganisms
may
encode
circRNA-like
molecules,
providing
new
insights
into
physiological
pathological
roles
circRNAs.
The
innate
immune
system
functions
as
body’s
primary
defense
mechanism
against
viral
infections.
It
detects
pathogen-associated
molecular
patterns
(PAMPs)
activates
signaling
pathways
to
suppress
replication
limit
their
spread.
CircRNAs
involved
regulation
host
play
essential
pathogenesis.
has
been
shown
circRNAs
can
regulate
gene
expression
by
acting
miRNA
sponges
protein
sponges,
encoding
small
proteins
specific
cases.
For
example,
previous
studies
revealed
participate
antiviral
response
through
competitive
endogenous
RNA
(ceRNA)
network
sponges.
This
review
highlights
research
progress
host-
virus-encoded
host-virus
interactions,
well
potential
diagnostic
biomarkers
therapeutic
targets
clinical
applications.
Avian Pathology,
Год журнала:
2024,
Номер
53(4), С. 229 - 241
Опубликована: Фев. 7, 2024
Marek's
disease
(MD)
is
a
neoplastic
that
significantly
affects
the
poultry
industry.
Long
non-coding
RNAs
(lncRNAs)
are
crucial
regulatory
factors
in
various
biological
processes,
including
tumourigenesis.
However,
involvement
of
novel
lncRNAs
course
MD
virus
(MDV)
infection
still
underexplored.
Here,
we
present
first
comprehensive
characterization
differentially
expressed
chicken
spleen
at
different
stages
MDV
infection.
A
series
was
identified
each
stage
through
screening.
Notably,
our
investigation
revealed
lncRNA,
lncRNA
803,
which
exhibited
significant
differential
expression
and
likely
to
be
associated
with
p53
pathway.
Further
analyses
demonstrated
overexpression
803
positively
regulated
TP53BP1
DF-1
cells,
leading
inhibition
apoptosis.
This
study
focus
on
profiles
spleens
during
pathogenesis.
Our
findings
highlight
potential
role
p53-related
pathogenesis
provide
valuable
insights
for
decoding
molecular
mechanism
involving
RNA.
Viruses,
Год журнала:
2023,
Номер
15(8), С. 1633 - 1633
Опубликована: Июль 27, 2023
The
host
response
to
pathogenic
microbes
can
lead
expression
of
interleukin
(IL)-17,
which
has
antimicrobial
and
anti-viral
activity.
However,
relatively
little
is
known
about
the
basic
biological
role
chicken
IL-17A
against
avian
viruses,
particularly
Marek’s
disease
virus
(MDV).
We
demonstrate
that,
following
MDV
infection,
upregulation
mRNA
an
increase
in
frequency
IL-17A+
T
cells
spleen
occur
compared
control
chickens.
To
elaborate
on
chIL-17A
MD,
full-length
coding
sequence
was
cloned
into
a
pCDNA3.1-V5/HIS
TOPO
plasmid.
effect
treatment
with
pcDNA:chIL-17A
plasmid
combination
vaccine
(HVT)
very
virulent(vv)MDV
challenge
or
vvMDV
infection
assessed.
In
HVT
vaccination,
chickens
that
were
inoculated
had
reduced
tumor
incidence
received
empty
vector
vaccinated
only
(66.6%
+
group
73.33%
versus
53.3%
pcDNA:chIL-17A).
Further
analysis
demonstrated
and/or
expressing
lower
MDV-Meq
transcripts
spleen.
conclusion,
influence
immunity
conferred
by
vaccination
Gene,
Год журнала:
2024,
Номер
910, С. 148331 - 148331
Опубликована: Март 3, 2024
Compelling
evidence
has
identified
circRNAs
as
crucial
regulators
in
initiation
and
progression
of
various
cancers,
including
gastric
cancer
(GC).
However,
the
function
regulatory
mechanisms
GC
remain
largely
unknown.
In
this
study,
attention
is
paid
to
a
novel
circular
RNA
circ1811,
which
exerts
significant
downregulated
expression
tissues
compared
with
adjacent
non-cancerous
tissues.
The
circ1811
tumor
negatively
correlated
extent
lymphatic
metastasis
patients.
Overexpression
inhibited
cell
proliferation,
migration
invasion
while
promoting
apoptosis,
whereas
knockdown
led
opposite
effects.
AGO2
RIP
dual
luciferase
reporter
assays
indicated
that
directly
sponges
miR-632
upregulate
DAPK1.
Collectively,
acts
tumor-suppressor
for
by
regulating
miR-632/DAPK1
axis.
Our
findings
suggest
potential
ideal
biomarker
therapeutic
target
GC.
Viruses,
Год журнала:
2023,
Номер
15(4), С. 817 - 817
Опубликована: Март 23, 2023
Marek’s
disease
(MD)
caused
by
pathogenic
virus
type
1
(MDV−1)
is
one
of
the
most
important
neoplastic
diseases
poultry.
MDV−1-encoded
unique
Meq
protein
major
oncoprotein
and
availability
Meq-specific
monoclonal
antibodies
(mAbs)
crucial
for
revealing
MDV
pathogenesis/oncogenesis.
Using
synthesized
polypeptides
from
conserved
hydrophilic
regions
as
immunogens,
together
with
hybridoma
technology
primary
screening
cross
immunofluorescence
assay
(IFA)
on
Meq-deleted
MDV−1
viruses
generated
CRISPR/Cas9-gene
editing,
a
total
five
positive
hybridomas
were
generated.
Four
these
hybridomas,
namely
2A9,
5A7,
7F9
8G11,
further
confirmed
to
secrete
specific
against
IFA
staining
293T
cells
overexpressing
Meq.
Confocal
microscopic
analysis
stained
nuclear
localization
in
MDV-infected
CEF
MDV-transformed
MSB-1
cells.
Furthermore,
two
mAb
clones,
2A9-B12
8G11-B2
derived
2A9
respectively,
displayed
high
specificity
proteins
strains
diverse
virulence.
Our
data
presented
here,
using
polypeptide
immunization
combined
CRISPR/Cas9
gene-edited
viruses,
has
provided
new
efficient
approach
future
generation
mAbs
viral
proteins.