A telomere-to-telomere cotton genome assembly reveals centromere evolution and a Mutator transposon-linked module regulating embryo development

Nature Genetics, Год журнала: 2024, Номер 56(9), С. 1953 - 1963

Опубликована: Авг. 15, 2024

Язык: Английский

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Large-scale long terminal repeat insertions produced a significant set of novel transcripts in cotton

Science China Life Sciences, Год журнала: 2023, Номер 66(8), С. 1711 - 1724

Опубликована: Апрель 17, 2023

Язык: Английский

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The cotton MYB33 gene is a hub gene regulating the trade-off between plant growth and defense in Verticillium dahliae infection

Journal of Advanced Research, Год журнала: 2023, Номер 61, С. 1 - 17

Опубликована: Авг. 28, 2023

Sessile plants engage in trade-offs between growth and defense capacity response to fluctuating environmental cues. MYB is an important transcription factor that plays many roles controlling plant defense. However, the mechanism behind how it keeps a balance these two physiological processes still largely unknown. Our work focuses on dissection of molecular by which GhMYB33 regulates The CRISPR/Cas9 technique was used generate mutants for deciphering functions. Yeast two-hybrid, luciferase complementary imaging, co-immunoprecipitation assays were prove proteins interact with each other. We electrophoretic mobility shift assay, yeast one-hybrid, activity analyze acting as promoter. A β-glucuronidase fusion reporter 5' RNA ligase mediated amplification cDNA ends analysis showed ghr-miR319c directedly cleaved mRNA. Overexpressing miR319c-resistant (rGhMYB33) promoted growth, accompanied significant decline resistance against Verticillium dahliae. Conversely, its knockout mutant, ghmyb33, demonstrated restriction concomitant augmentation V. dahliae resistance. found couple DELLA protein GhGAI1 bind specific cis-elements GhSPL9 GhDFR1 promoters, thereby modulating internode elongation infection. discovered target suppress expression. overexpression led enhanced simultaneous reduction height. findings demonstrate encodes hub controls expression GhDFR1, implicating pivotal role miR319c-MYB33 module regulate

Язык: Английский

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Crop antiviral defense: Past and future perspective

Science China Life Sciences, Год журнала: 2024, Номер 67(12), С. 2617 - 2634

Опубликована: Авг. 15, 2024

Язык: Английский

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Cotton sphingosine kinase GhLCBK1 participates in fiber cell elongation by affecting sphingosine-1-phophate and auxin synthesis

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 267, С. 131323 - 131323

Опубликована: Апрель 2, 2024

Sphingolipids serve as essential components of biomembrane and possess significant bioactive properties. Sphingosine-1-phophate (S1P) plays a key role in plant resistance to stress, but its specific impact on growth development remains be fully elucidated. Cotton fiber cells are an ideal material for investigating the maturation cells. In this study, we examined content composition sphingosine (Sph) S1P throughout progression cell development. The elevated gradually during elongation declined transition stage. Exogenous application promoted while using FTY720 (an antagonist S1P), DMS inhibitor LCBK) hindered elongation. Long Chain Base Kinase 1 (GhLCBK1) was notably expressed stage, containing all conserved domains LCBK protein localized endoplasmic reticulum. Overexpression GhLCBK1 increased retarded secondary wall (SCW) deposition. Conversely, downregulation reduced levels, suppressed elongation, accelerated SCW Transcriptome analysis revealed that upregulating or applying induced expression GhEXPANSIN auxin related genes. Furthermore, levels IAA were fibers when up-regulating down-regulating GhLCBK1, respectively. Our investigation demonstrated product facilitated by affecting biosynthesis. This study contributes novel insights into intricate regulatory pathways involved identifying potential target gene laying groundwork enhancing quality via genetic manipulation.

Язык: Английский

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Genome-wide identification and expression-pattern analysis of sulfate transporter (SULTR) gene family in cotton under multiple abiotic stresses and fiber development

Functional & Integrative Genomics, Год журнала: 2024, Номер 24(3)

Опубликована: Май 22, 2024

Язык: Английский

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Genome‑wide Characterization of the cotton FAR gene family reveals a positive role of GhFAR3 in Verticillium dahliae resistance

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Fatty acyl-CoA reductase (FAR) proteins play a crucial role in plant growth and development. Previous studies on FAR primarily focused their involvement the synthesis of suberin waxes, while limited attention has been given to roles biotic abiotic stress responses. In this study, 31 FAR genes were identified across Gossypium arboreum, G. raimondii, hirsutum, barbadense. Bioinformatics analyses revealed that cotton cluster into three distinct groups, with conserved motifs, exon-coding sequences, domains among within same group. Promoter analysis numerous cis-acting elements associated hormone regulation GhFAR genes, highlighting regulatory potential. Gene family expansion was predominantly driven by whole-genome duplication events. Expression profiling demonstrated are expressed various tissues, most showing reduced expression under stresses but significantly increased following Verticillium dahliae infection. Functional using virus-induced gene silencing (VIGS) GhFAR3 its compromises disease resistance reducing accumulation roots. This study provides comprehensive genome-wide identification characterization cotton, emphasizing potential biological functions. Furthermore, it establishes as key player enhancing V. dahliae, mediated through biosynthesis

Язык: Английский

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Utilizing physiologies, transcriptomics, and metabolomics to unravel key genes and metabolites of Salvia miltiorrhiza Bge. seedlings in response to drought stress

Frontiers in Plant Science, Год журнала: 2025, Номер 15

Опубликована: Янв. 14, 2025

Drought stress inhibits Salvia miltiorrhiza Bunge (S. miltiorrhiza) seedling growth and yield. Here, we studied the effects of drought on different parts S. seedlings through physiological, transcriptomic, metabolomics analyses, identified key genes metabolites related to tolerance. Physiological analysis showed that increased accumulation hydrogen peroxide (H2O2), enhanced activity peroxidase (POD), decreased catalase (CAT) contents chlorophyll b total chlorophyll, reduced degree photosynthesis, oxidative damage in seedlings, inhibited plants. Transcriptome analyses revealed 383 encoding transcription factors 80 plant hormones as hypothetical regulators resistance Moreover, differentially expressed (DEGs) (DEMs) are involved a variety biological processes, such proline glycine betaine metabolism, biosynthesis tanshinones phenolic acids. Additionally, it has barely been reported AHL gene family may be regulating neocryptotanshinone biosynthesis. In conclusion, our results suggest by enhancing membrane lipid peroxidation, attenuating antioxidant system, hormones, acid metabolism pathways. This study provides new insights into complex mechanisms which responds stress.

Язык: Английский

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Genome-wide identification of SERKs in four Gossypium species reveals GhBAK1 potentially promotes cotton defoliation

Industrial Crops and Products, Год журнала: 2025, Номер 226, С. 120681 - 120681

Опубликована: Фев. 18, 2025

Язык: Английский

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GhMYB102 affects cotton fibre elongation and secondary wall thickening by regulating GhIRX10 in cotton

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Фев. 18, 2025

Summary Upland cotton ( Gossypium hirsutum ) is a principal economic crop and fundamental raw material for the textile industry. The quality of fibres significantly influenced by synthesis cell wall polysaccharides. This study focuses on GhIRX10 , beta‐1,4‐xylosyltransferase crucial xylan backbone synthesis. Overexpression enhances synthesis, which impacts fibre elongation secondary thickening. GhMYB102 identified as direct regulator expression, was confirmed through comprehensive validation. resulted in similar phenotype OE‐ : increased thickness reduced length. upregulated expression key synthesis‐related genes, including GhCESA4/7/8 Gh IRXs GhCESAs GUXs TBLs XTHs XXTs . Consequently, cellulose hemicellulose contents lines were increased. also validated target gene regulated GhFSN1 GhMYB7 with ability to reciprocally regulate expression. In summary, we propose regulatory model where promotes other wall‐related thereby affecting quality. elucidates network provides potential targets improving molecular breeding.

Язык: Английский

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