Crop antiviral defense: Past and future perspective DOI
Zhirui Yang, Guangyao Li, Yongliang Zhang

et al.

Science China Life Sciences, Journal Year: 2024, Volume and Issue: 67(12), P. 2617 - 2634

Published: Aug. 15, 2024

Language: Английский

GbPP2C80 Interacts with GbWAKL14 to Negatively Co‐Regulate Resistance to Fusarium and Verticillium wilt via MPK3 and ROS Signaling in Sea Island Cotton DOI Creative Commons
Nan Zhao, Anhui Guo, Weiran Wang

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)

Published: June 18, 2024

Fusarium wilt (FW) is widespread in global cotton production, but the mechanism underlying FW resistance superior-fiber-quality Sea Island unclear. This study reveals that has been target of genetic improvement China since 2010s. The key nonsynonymous single nucleotide polymorphism (SNP, T/C) gene Gbar_D03G001670 encoding protein phosphatase 2C 80 (PP2C80) results an amino acid shift (L/S), which significantly associated with cotton. Silencing GbPP2C80 increases cotton, whereas overexpressing reduces Arabidopsis. and GbWAKL14 exist synergistically accessions haplotype forms "susceptible-susceptible" (TA) "resistant-resistant" (CC), interact each other. CRISPR/Cas9-mediated knockout enhances Verticillium (VW) upland overexpression weakens VW respond to by modulating reactive oxygen species (ROS) content via affecting MPK3 expression. In summary, two tandem genes on chromosome D03, GbPP2C80, GbWAKL14, functions as cooperative negative regulators disease defense, providing novel resources molecular markers for development resistant cultivars.

Language: Английский

Citations

4
A telomere-to-telomere cotton genome assembly reveals centromere evolution and a Mutator transposon-linked module regulating embryo development DOI
Gai Huang, Zhigui Bao, Feng Li

et al.

Nature Genetics, Journal Year: 2024, Volume and Issue: 56(9), P. 1953 - 1963

Published: Aug. 15, 2024

Language: Английский

Citations

4
Genome‑wide Characterization of the cotton FAR gene family reveals a positive role of GhFAR3 in Verticillium dahliae resistance DOI Creative Commons

Wenhan Cheng,

Siqi Gao,

Zhaojie He

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 2, 2025

Abstract Fatty acyl-CoA reductase (FAR) proteins play a crucial role in plant growth and development. Previous studies on FAR primarily focused their involvement the synthesis of suberin waxes, while limited attention has been given to roles biotic abiotic stress responses. In this study, 31 FAR genes were identified across Gossypium arboreum, G. raimondii, hirsutum, barbadense. Bioinformatics analyses revealed that cotton cluster into three distinct groups, with conserved motifs, exon-coding sequences, domains among within same group. Promoter analysis numerous cis-acting elements associated hormone regulation GhFAR genes, highlighting regulatory potential. Gene family expansion was predominantly driven by whole-genome duplication events. Expression profiling demonstrated are expressed various tissues, most showing reduced expression under stresses but significantly increased following Verticillium dahliae infection. Functional using virus-induced gene silencing (VIGS) GhFAR3 its compromises disease resistance reducing accumulation roots. This study provides comprehensive genome-wide identification characterization cotton, emphasizing potential biological functions. Furthermore, it establishes as key player enhancing V. dahliae, mediated through biosynthesis

Language: Английский

Citations

0
Utilizing physiologies, transcriptomics, and metabolomics to unravel key genes and metabolites of Salvia miltiorrhiza Bge. seedlings in response to drought stress DOI Creative Commons
Yu Zhang, Hong Zhang, Yuru Zhang

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 14, 2025

Drought stress inhibits Salvia miltiorrhiza Bunge (S. miltiorrhiza) seedling growth and yield. Here, we studied the effects of drought on different parts S. seedlings through physiological, transcriptomic, metabolomics analyses, identified key genes metabolites related to tolerance. Physiological analysis showed that increased accumulation hydrogen peroxide (H2O2), enhanced activity peroxidase (POD), decreased catalase (CAT) contents chlorophyll b total chlorophyll, reduced degree photosynthesis, oxidative damage in seedlings, inhibited plants. Transcriptome analyses revealed 383 encoding transcription factors 80 plant hormones as hypothetical regulators resistance Moreover, differentially expressed (DEGs) (DEMs) are involved a variety biological processes, such proline glycine betaine metabolism, biosynthesis tanshinones phenolic acids. Additionally, it has barely been reported AHL gene family may be regulating neocryptotanshinone biosynthesis. In conclusion, our results suggest by enhancing membrane lipid peroxidation, attenuating antioxidant system, hormones, acid metabolism pathways. This study provides new insights into complex mechanisms which responds stress.

Language: Английский

Citations

0
Genome-wide identification of SERKs in four Gossypium species reveals GhBAK1 potentially promotes cotton defoliation DOI Creative Commons
Na Qin,

Yuan Tian,

Abdul Rehman

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120681 - 120681

Published: Feb. 18, 2025

Language: Английский

Citations

0
GhMYB102 affects cotton fibre elongation and secondary wall thickening by regulating GhIRX10 in cotton DOI Creative Commons
Aimin Wu, Tong Shen,

Jianhua Lü

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

Summary Upland cotton ( Gossypium hirsutum ) is a principal economic crop and fundamental raw material for the textile industry. The quality of fibres significantly influenced by synthesis cell wall polysaccharides. This study focuses on GhIRX10 , beta‐1,4‐xylosyltransferase crucial xylan backbone synthesis. Overexpression enhances synthesis, which impacts fibre elongation secondary thickening. GhMYB102 identified as direct regulator expression, was confirmed through comprehensive validation. resulted in similar phenotype OE‐ : increased thickness reduced length. upregulated expression key synthesis‐related genes, including GhCESA4/7/8 Gh IRXs GhCESAs GUXs TBLs XTHs XXTs . Consequently, cellulose hemicellulose contents lines were increased. also validated target gene regulated GhFSN1 GhMYB7 with ability to reciprocally regulate expression. In summary, we propose regulatory model where promotes other wall‐related thereby affecting quality. elucidates network provides potential targets improving molecular breeding.

Language: Английский

Citations

0
The DNA N6‐adenine methylation target gene GhMAF1 promotes fiber initiation at the base of Gossypium ovules DOI

Yanfei Pei,

Yongming Liu,

Jun Peng

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Summary DNA methylation consists of 5‐methylcytosine and N6‐methyl deoxyadenosine (6mA) is crucial in plant development. However, its specific role potential mechanism to initiate cotton fibers remain unclear. This study employed Oxford Nanopore Technologies (ONT) sequencing analyze alterations ZM24 fuzzless‐lintless (ZM24 fl ) during fiber initiation. Our results indicated that 6mA exhibited the most remarkable difference among ovule samples at −2, 0, 5 d post anthesis . Subsequently, genes with significant changes transcription initiation were screened. We found GhMAF1 displayed transcriptional upregulation enrichment promoter, which could serve as a target for Further, we knocked out using CRISPR‐Cas technology demonstrated specifically promotes cells base by mediating downstream JAZ2/CPC‐MML3/MML4 pathway. These findings unveil novel spatial module cell on surface involves Ultimately, this work provides knowledge regulatory network modification improve yield quality.

Language: Английский

Citations

0
A telomere-to-telomere genome assembly of cotton provides insights into centromere evolution and short-season adaptation DOI
Guanjing Hu, Zhenyu Wang,

Zunzhe Tian

et al.

Nature Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

Citations

0
SEEDSTICK Affects Seed Development by Mediating Cytokinin Levels in Cotton DOI
Xiaohong Zhang, Yi-Fan Li, Y. C. Ge

et al.

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

The SEEDSTICK transcription factor is important for flower and seed development, but the underlying molecular mechanisms remain unclear in cotton. In this study, we identified cloned two STK homolog genes upland cotton, an economically valuable cultivated crop. Phylogenetic sequence analyses showed that C-terminus of both GhSTKs had a conserved -DJJILHLG amino acid GhSTK1 GhSTK2 were very similar to GaAGL11 GrAGL11, respectively. Quantitative real-time PCR analysis revealed highly expressed ovules, GUS activity was detected style stigma. Subcellular localization experiments localized nucleus. Arabidopsis, overexpression or affected floral organ development formation by increasing transcript levels CKX other related development. Silencing increased expression GhFT GhSHP led earlier appearance cotton buds. Yeast two-hybrid bimolecular fluorescence complementation assays indicated GhSTK proteins could interact with GhSEP3 GhSEP4 proteins. present results suggest GhSTK2, which have different sequences patterns, might be functionally redundant influence regulation bud

Language: Английский

Citations

0
Comparative single-cell transcriptomic map reveals divergence in leaves between two cotton species at cell type resolution DOI Creative Commons

Hailiang Cheng,

Shang Liu, Youping Zhang

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0