Enhancements in Cotton Fiber Length Phenotyping and Optimization of Weighted Gene Co-expression Network Analysis through Fiber Elongation Rate Metrics DOI Creative Commons

Leidi Liu,

Cheng Li, Feng Hu

и другие.

New Crops, Год журнала: 2025, Номер unknown, С. 100080 - 100080

Опубликована: Май 1, 2025

Язык: Английский

The DNA N6‐adenine methylation target gene GhMAF1 promotes fiber initiation at the base of Gossypium ovules DOI

Yanfei Pei,

Yongming Liu,

Jun Peng

и другие.

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Summary DNA methylation consists of 5‐methylcytosine and N6‐methyl deoxyadenosine (6mA) is crucial in plant development. However, its specific role potential mechanism to initiate cotton fibers remain unclear. This study employed Oxford Nanopore Technologies (ONT) sequencing analyze alterations ZM24 fuzzless‐lintless (ZM24 fl ) during fiber initiation. Our results indicated that 6mA exhibited the most remarkable difference among ovule samples at −2, 0, 5 d post anthesis . Subsequently, genes with significant changes transcription initiation were screened. We found GhMAF1 displayed transcriptional upregulation enrichment promoter, which could serve as a target for Further, we knocked out using CRISPR‐Cas technology demonstrated specifically promotes cells base by mediating downstream JAZ2/CPC‐MML3/MML4 pathway. These findings unveil novel spatial module cell on surface involves Ultimately, this work provides knowledge regulatory network modification improve yield quality.

Язык: Английский

Процитировано

0
A telomere-to-telomere genome assembly of cotton provides insights into centromere evolution and short-season adaptation DOI
Guanjing Hu, Zhenyu Wang,

Zunzhe Tian

и другие.

Nature Genetics, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Язык: Английский

Процитировано

0
SEEDSTICK Affects Seed Development by Mediating Cytokinin Levels in Cotton DOI
Xiaohong Zhang, Yi-Fan Li, Y. C. Ge

и другие.

Physiologia Plantarum, Год журнала: 2025, Номер 177(2)

Опубликована: Март 1, 2025

The SEEDSTICK transcription factor is important for flower and seed development, but the underlying molecular mechanisms remain unclear in cotton. In this study, we identified cloned two STK homolog genes upland cotton, an economically valuable cultivated crop. Phylogenetic sequence analyses showed that C-terminus of both GhSTKs had a conserved -DJJILHLG amino acid GhSTK1 GhSTK2 were very similar to GaAGL11 GrAGL11, respectively. Quantitative real-time PCR analysis revealed highly expressed ovules, GUS activity was detected style stigma. Subcellular localization experiments localized nucleus. Arabidopsis, overexpression or affected floral organ development formation by increasing transcript levels CKX other related development. Silencing increased expression GhFT GhSHP led earlier appearance cotton buds. Yeast two-hybrid bimolecular fluorescence complementation assays indicated GhSTK proteins could interact with GhSEP3 GhSEP4 proteins. present results suggest GhSTK2, which have different sequences patterns, might be functionally redundant influence regulation bud

Язык: Английский

Процитировано

0
Comparative single-cell transcriptomic map reveals divergence in leaves between two cotton species at cell type resolution DOI Creative Commons

Hailiang Cheng,

Shang Liu, Youping Zhang

и другие.

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Enhancements in Cotton Fiber Length Phenotyping and Optimization of Weighted Gene Co-expression Network Analysis through Fiber Elongation Rate Metrics DOI Creative Commons

Leidi Liu,

Cheng Li, Feng Hu

и другие.

New Crops, Год журнала: 2025, Номер unknown, С. 100080 - 100080

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0