Science China Life Sciences, Journal Year: 2024, Volume and Issue: 67(12), P. 2617 - 2634
Published: Aug. 15, 2024
Language: Английский
New Crops, Journal Year: 2025, Volume and Issue: unknown, P. 100080 - 100080
Published: May 1, 2025
Language: Английский
Citations
0
International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 143662 - 143662
Published: April 1, 2025
Language: Английский
Citations
0
Journal of Advanced Research, Journal Year: 2023, Volume and Issue: 61, P. 1 - 17
Published: Aug. 28, 2023
Sessile plants engage in trade-offs between growth and defense capacity response to fluctuating environmental cues. MYB is an important transcription factor that plays many roles controlling plant defense. However, the mechanism behind how it keeps a balance these two physiological processes still largely unknown. Our work focuses on dissection of molecular by which GhMYB33 regulates The CRISPR/Cas9 technique was used generate mutants for deciphering functions. Yeast two-hybrid, luciferase complementary imaging, co-immunoprecipitation assays were prove proteins interact with each other. We electrophoretic mobility shift assay, yeast one-hybrid, activity analyze acting as promoter. A β-glucuronidase fusion reporter 5' RNA ligase mediated amplification cDNA ends analysis showed ghr-miR319c directedly cleaved mRNA. Overexpressing miR319c-resistant (rGhMYB33) promoted growth, accompanied significant decline resistance against Verticillium dahliae. Conversely, its knockout mutant, ghmyb33, demonstrated restriction concomitant augmentation V. dahliae resistance. found couple DELLA protein GhGAI1 bind specific cis-elements GhSPL9 GhDFR1 promoters, thereby modulating internode elongation infection. discovered target suppress expression. overexpression led enhanced simultaneous reduction height. findings demonstrate encodes hub controls expression GhDFR1, implicating pivotal role miR319c-MYB33 module regulate
Language: Английский
Citations
10
International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 267, P. 131323 - 131323
Published: April 2, 2024
Sphingolipids serve as essential components of biomembrane and possess significant bioactive properties. Sphingosine-1-phophate (S1P) plays a key role in plant resistance to stress, but its specific impact on growth development remains be fully elucidated. Cotton fiber cells are an ideal material for investigating the maturation cells. In this study, we examined content composition sphingosine (Sph) S1P throughout progression cell development. The elevated gradually during elongation declined transition stage. Exogenous application promoted while using FTY720 (an antagonist S1P), DMS inhibitor LCBK) hindered elongation. Long Chain Base Kinase 1 (GhLCBK1) was notably expressed stage, containing all conserved domains LCBK protein localized endoplasmic reticulum. Overexpression GhLCBK1 increased retarded secondary wall (SCW) deposition. Conversely, downregulation reduced levels, suppressed elongation, accelerated SCW Transcriptome analysis revealed that upregulating or applying induced expression GhEXPANSIN auxin related genes. Furthermore, levels IAA were fibers when up-regulating down-regulating GhLCBK1, respectively. Our investigation demonstrated product facilitated by affecting biosynthesis. This study contributes novel insights into intricate regulatory pathways involved identifying potential target gene laying groundwork enhancing quality via genetic manipulation.
Language: Английский
Citations
3
Science China Life Sciences, Journal Year: 2024, Volume and Issue: 67(12), P. 2617 - 2634
Published: Aug. 15, 2024
Language: Английский
Citations
3