Role of Chinese Medicine Monomers in Dry Eye Disease: Breaking the Vicious Cycle of Inflammation
Pharmacology Research & Perspectives,
Год журнала:
2025,
Номер
13(2)
Опубликована: Фев. 20, 2025
Dry
eye
disease
(DED)
is
a
chronically
inflammatory
ocular
surface
disorder
of
unknown
pathogenesis.
Anti-inflammatory
medications,
artificial
tears,
autologous
serum,
and
LipiFlow
have
been
shown
to
be
highly
beneficial
in
alleviating
symptoms.
Nevertheless,
these
interventions
often
provide
only
short-term
results
do
not
address
the
underlying
problems
disease.
There
growing
evidence
that
risk
DED
associated
with
vicious
cycle
inflammation.
This
inflammation
produced
by
interaction
several
factors,
including
tear
film
hyperosmolarity,
instability,
inflammation,
apoptosis.
Chinese
medicine
monomers,
distinguished
their
multicomponent
multitarget
advantages,
help
treat
modulating
status,
inhibiting
responses,
apoptosis,
providing
new
way
thinking
management
medicine.
Язык: Английский
Network Pharmacology-Based Strategy Integrated with Molecular Docking and In Vitro Experimental Validation to Explore the Underlying Mechanism of Fangji Huangqi Decoction in Treating Rheumatoid Arthritis
ACS Omega,
Год журнала:
2024,
Номер
9(29), С. 31878 - 31889
Опубликована: Июль 10, 2024
decoction
(FHD),
as
a
classic
traditional
Chinese
medicine
formula,
has
been
clinically
proven
effective
against
rheumatoid
arthritis
(RA),
yet
its
therapeutic
mechanism
remains
unclear.
This
study
employed
network
pharmacology
and
molecular
docking
methods
to
explore
the
major
active
components,
biological
targets,
signaling
pathways
of
FHD.
Subsequently,
lipopolysaccharide
(LPS)-stimulated
RAW264.7
cells
were
used
in
vitro
model
validate
modulating
effects
FHD
on
molecules/inflammatory
mediators
using
various
biomedical
techniques/kits
such
MTT
assay,
Griess
reagents,
flow
cytometry,
RT-qPCR,
immunoblotting.
Network
analyses
indicated
total
20
components
30
core
targets
RA.
Pathway
enrichment
demonstrated
involvement
mitogen-activated
protein
kinase
(MAPK)
efficacy
formula.
Furthermore,
experimental
evidence
that
dose-dependently
significantly
inhibited
productions
nitric
oxide
(NO)
reactive
oxygen
species;
lowered
mRNA
expression
levels
proinflammatory
including
iNOS,
COX-2,
TNF-α,
ΙL-1β,
IL-6;
decreased
phosphorylated
forms
p38,
ERK,
JNK,
NF-κB
p65.
Additionally,
results
showed
tetrandrine,
licochalcone
A,
oxonantenine,
isorhamnetin,
kaempferol
exerted
potent
capability
binding
target
molecules
focused
pathway,
probably
being
potential
substances
for
Our
integrated
with
cellular
validation
elucidated
exerts
downregulating
MAPK
ultimately
leading
inhibitory
LPS-stimulated
cells.
work
comprehensively
substances,
key
involved
anti-RA
these
findings
provide
further
understanding
underlying
managing
Язык: Английский
Pro-differentiative, Pro-adhesive and Pro-migratory Activities of Isorhamnetin in MC3T3-E1 Osteoblasts via Activation of ERK-dependent BMP2-Smad Signaling
Cell Biochemistry and Biophysics,
Год журнала:
2024,
Номер
82(4), С. 3607 - 3617
Опубликована: Авг. 13, 2024
Язык: Английский
Chromosome‐level genome assembly of Iodes seguinii and its metabonomic implications for rheumatoid arthritis treatment
The Plant Genome,
Год журнала:
2024,
Номер
18(1)
Опубликована: Ноя. 27, 2024
Abstract
Iodes
seguinii
is
a
woody
vine
known
for
its
potential
therapeutic
applications
in
treating
rheumatoid
arthritis
(RA)
due
to
rich
bioactive
components.
Here,
we
achieved
the
first
chromosome‐level
assembly
of
nuclear
genome
I.
using
PacBio
HiFi
and
chromatin
conformation
capture
(Hi‐C)
sequencing
data.
The
initial
with
data
produced
contigs
an
N50
length
9.71
Mb,
Hi‐C
anchored
these
into
13
chromosomes,
achieving
total
273.58
closely
matching
estimated
size.
Quality
assessments,
including
BUSCO,
long
terminal
repeat
index,
transcriptome
mapping
rates,
coverage,
confirmed
high
quality,
completeness,
continuity
assembly,
identifying
115.28
Mb
repetitive
sequences,
1062
RNA
genes,
25,270
protein‐coding
genes.
Additionally,
assembled
annotated
150,599
bp
chloroplast
Illumina
data,
containing
121
genes
key
DNA
barcodes,
maturase
K
(
matK
)
proving
effective
species
identification.
Phylogenetic
analysis
positioned
at
base
Lamiales
clade,
significant
gene
family
expansions
contractions,
particularly
related
secondary
metabolite
synthesis
damage
repair.
Metabolite
identified
84
active
components
,
discovery
luteolin,
119
targets
predicted
RA
treatment,
core
like
AKT1
toll‐like
receptor
4
TLR4
),
epidermal
growth
factor
EGFR
tumor
necrosis
TNF
TP53
NFKB1
janus
kinase
2
JAK2
BCL2
mitogen‐activated
protein
1
MAPK1
spleen‐associated
tyrosine
SYK
).
Key
such
as
flavonoids
polyphenols
anti‐inflammatory
activities
were
highlighted.
particular,
underscores
role.
These
findings
provide
valuable
genomic
resource
scientific
basis
development
application
addressing
gap
genus
order
Icacinales
underscoring
need
further
research
genomics,
transcriptomics,
metabolomics
fully
explore
potential.
Язык: Английский
From Tea to Functional Foods: Exploring Caryopteris mongolica Bunge for Anti-Rheumatoid Arthritis and Unraveling Its Potential Mechanisms
Nutrients,
Год журнала:
2024,
Номер
16(24), С. 4311 - 4311
Опубликована: Дек. 13, 2024
Background:
Caryopteris
mongolica
Bunge
(CM)
shows
promising
potential
for
managing
rheumatoid
arthritis
(RA)
and
digestive
disorders,
attributed
to
its
rich
content
of
bioactive
compounds
such
as
polyphenols
flavonoids.
Despite
common
use
in
herbal
tea,
the
specific
mechanisms
underlying
CM’s
anti-inflammatory
joint-protective
effects
remain
unclear,
limiting
development
a
functional
food.
This
study
investigated
aqueous
CM
extract
on
RA
collagen-induced
(CIA)
rats
explored
mechanisms.
Methods:
Forty-eight
female
Sprague-Dawley
were
randomly
assigned
six
groups
(n
=
8):
normal
control,
CIA
model,
methotrexate
(MTX),
high-,
middle-,
low-dose
groups.
Anti-inflammatory
evaluated
using
biochemical
histological
analyses.
To
elucidate
mechanisms,
we
applied
metabolomics,
network
pharmacology,
transcriptomics
approaches.
Results:
The
results
demonstrated
that
effectively
suppressed
synovial
inflammation
rats,
reducing
joint
degradation.
mediated
through
TNF
signaling
pathway,
modulating
glycerophospholipid
amino
acid
metabolism,
including
reduced
levels
tryptophan,
LysoPC,
asparagine.
Molecular
docking
identified
scutellarin
apigenin
key
compounds.
Additionally,
immunofluorescence
analysis
revealed
therapeutic
via
inhibition
suppression
M1
macrophage
polarization.
Conclusions:
These
findings
highlight
support
food
or
pharmaceutical
product.
Язык: Английский
Synergistic metabolic modulation of fibroblast-like synoviocytes via targeted dual prodrug nanoparticles to mitigate rheumatoid arthritis
Acta Pharmaceutica Sinica B,
Год журнала:
2024,
Номер
15(1), С. 542 - 556
Опубликована: Ноя. 18, 2024
Elevated
glucose
metabolism
is
a
prominent
characteristic
of
fibroblast-like
synoviocytes
(FLS)
in
rheumatoid
arthritis
(RA).
However,
the
efficacy
inhibiting
single
target
FLS
using
small
molecular
inhibitors
limited
for
RA
treatment.
Herein,
synergistic
inhibition
FLS'
survival,
proliferation,
and
activation
by
combining
two
inhibitors,
diclofenac
(DC)
lonidamine
(LND)
was
first
verified.
Subsequently,
DC
LND
were
individually
conjugated
to
cystamine-modified
hyaluronic
acid
(HA)
prepare
polymer-prodrug
conjugates.
A
HAP-1
peptide-modified
dual
conjugates-assembled
nanoparticles
system
(HAP-1NPDC+LND)
further
tailored
optimal
ratio
targeted
metabolic
modulation
alleviate
symptoms.
Upon
uptake
inflamed
joints,
HAP-1NPDC+LND
released
within
intracellular
reductive
microenvironment,
where
hinders
suppresses
glycolytic
enzymes
eliminate
synergistically.
Additionally,
secretion
lactic
pro-inflammatory
factors
from
reduced,
thereby
disrupting
crosstalk
between
macrophages.
Finally,
demonstrated
promising
mouse
model
collagen-induced
(CIA).
Overall,
this
research
provides
valuable
insights
into
novel
therapeutic
strategies
safe
effective
treatment
through
FLS.
Язык: Английский