Cultivated
meat
has
attracted
widespread
attention
as
a
promising
technology
with
potential
benefits
in
food
safety,
sustainable
development,
and
traceable
origin.
While
countries
worldwide
invest
significantly
research
on
cultivated
meat,
fundamental
challenge
lies
efficiently
simulating
cellular
growth
enabling
large-scale
production
bioreactors.
Despite
advances
tissue
engineering,
achieving
cost-effective
technically
viable
remains
complex.
Although
animal
cell
culture
media
been
refined,
it
lacks
specific
adaptations
to
meet
the
unique
requirements
of
meat.
Successful
requires
food-grade
minimal
cost,
regulation
proliferation
differentiation,
creation
an
acceptable
product
sensory
qualities,
animal-free
origin,
adhering
regulations
for
media.
The
formulation
these
qualities
draws
inspiration
from
conventional
applications
principles
used
optimizing
microbial
fermentation
processes,
producing
ingredients
lower
value
at
scale.
Until
now,
analysis
concludes
that
improvements
metabolic
efficiency
development
low-cost
serum-free
are
still
insufficient
assume
substitution
by
Frontiers in Bioscience-Landmark,
Год журнала:
2025,
Номер
30(3)
Опубликована: Март 18, 2025
Background:
Cultured
meat
holds
significant
potential
as
a
pivotal
solution
for
producing
safe,
sustainable,
and
high-quality
protein
to
meet
the
growing
demands
of
global
population.
However,
scaling
this
technology
requires
innovative
bioengineering
approaches
integrated
with
software
methods
assess
growth
cell
cultures.
This
study
aims
develop
3D
printing
hybrid
product
subsequently
design
finely
tuned
You
Only
Look
Once
(YOLO)
model
detecting
counting
lipoblasts,
fibroblasts,
myogenic
cells.
Methods:
Cultures
multipotent
mesenchymal
stem
cells
(MMSCs)
fibroblasts
were
obtained
from
domestic
rabbit
Oryctolagus
cuniculus
domesticus.
Standard
protocols
employed
induce
adipogenic
differentiation
MMSCs.
Fibroblasts
isolated
skin
biopsy
samples.
The
process
utilized
bioinks.
engineering
approach
involved
development
unique
print
head
into
printer.
Confocal
transmission
electron
microscopy
within
construct
was
performed.
A
dataset
digital
images
cells,
created.
Four
models
based
on
YOLOv8-seg
architecture
trained
annotated
images,
implemented
in
Telegram
bot.
Results:
Stable
cultures
obtained.
3D-printed
tissue
constructs
composed
sodium
alginate,
sunflower
successfully
fabricated.
printer
assembled.
confirmed
viability
construct.
Ultrastructural
analysis
revealed
dense
intercellular
contacts
high
metabolic
activity.
resulting
replicated
organoleptic
structural
properties
natural
meat.
In
IT
segment,
single-class
lipoblasts
achieved
metrics
recall
85%,
precision
77%,
mean
Average
Precision
at
IoU
threshold
0.50
(mAP50)
79%,
which
improved
multiclass
92%,
mAP50
81%.
bot
capable
different
types.
Conclusions:
achieved.
Detailed
microscopic
demonstrated
activity
polymerized
alginate
hydrogel.
engineered
presents
alternative
Additionally,
neural
network
models,
bot,
proved
effective
monitoring
culture
identifying
types
across
three
As
result,
we
developed
four
YOLOv8
that
outperforms
model.
all
exhibited
reduced
accuracy
high-density
cultures,
where
overlapping
led
undercounting.
Cultivated
meat
has
attracted
widespread
attention
as
a
promising
technology
with
potential
benefits
in
food
safety,
sustainable
development,
and
traceable
origin.
While
countries
worldwide
invest
significantly
research
on
cultivated
meat,
fundamental
challenge
lies
efficiently
simulating
cellular
growth
enabling
large-scale
production
bioreactors.
Despite
advances
tissue
engineering,
achieving
cost-effective
technically
viable
remains
complex.
Although
animal
cell
culture
media
been
refined,
it
lacks
specific
adaptations
to
meet
the
unique
requirements
of
meat.
Successful
requires
food-grade
minimal
cost,
regulation
proliferation
differentiation,
creation
an
acceptable
product
sensory
qualities,
animal-free
origin,
adhering
regulations
for
media.
The
formulation
these
qualities
draws
inspiration
from
conventional
applications
principles
used
optimizing
microbial
fermentation
processes,
producing
ingredients
lower
value
at
scale.
Until
now,
analysis
concludes
that
improvements
metabolic
efficiency
development
low-cost
serum-free
are
still
insufficient
assume
substitution
by
