RETRACTED: Cellular functions of spermatogonial stem cells in relation to JAK/STAT signaling pathway

Frontiers in Cell and Developmental Biology, Год журнала: 2024, Номер 11

Опубликована: Фев. 13, 2024

This manuscript comprehensively reviews the interrelationship between spermatogonial stem cells (SSCs) and JAK/STAT signaling pathway. Spermatogonial in testes of male mammals, characterized by their self-renewal pluripotential differentiation capabilities, are essential for tissue regeneration, immunomodulation, advancements regenerative medicine. review delves into historical background biological characteristics SSCs, with a particular emphasis on pivotal role pathway proliferation, maturation, processes. Research indicates that extensively influences various functionalities cells, encompassing differentiation, homing, adaptation to microenvironment. Herein, we collate dissect related studies, shedding light intricate dynamics SSCs pathway, examine implications these interactions attributes SSCs. Furthermore, discusses profound findings preclinical research domain cellular engineering. It is acknowledged that, despite investigations humans larger mammals remain inadequate, necessitating more in-depth exploration establish comprehensive theoretical framework. Overall, this offers an invaluable reference deciphering mechanisms cell pathways establishes groundwork research.

Язык: Английский

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Whole transcriptome analysis to identify non-coding RNA regulators and hub genes in sperm of non-obstructive azoospermia by microarray, single-cell RNA sequencing, weighted gene co-expression network analysis, and mRNA-miRNA-lncRNA interaction analysis

BMC Genomics, Год журнала: 2024, Номер 25(1)

Опубликована: Июнь 11, 2024

Abstract Background The issue of male fertility is becoming increasingly common due to genetic differences inherited over generations. Gene expression and evaluation non-coding RNA (ncRNA), crucial for sperm development, are significant factors. This gene can affect motility and, consequently, fertility. Understanding the intricate protein interactions that play essential roles in differentiation development vital. knowledge could lead more effective treatments interventions infertility. Materials methods Our research aim identify new key genes ncRNA involved non-obstructive azoospermia (NOA), improving diagnosis offering accurate estimates successful extraction based on an individual’s genotype. Results We analyzed transcript three NOA patients who tested negative issues, employing comprehensive genome-wide analysis approximately 50,000 sequences using microarray technology. compared profiles between normal sperm. found differences: 150 were up-regulated, 78 down-regulated, along with 24 ncRNAs up-regulated 13 down-regulated conditions. By cross-referencing our results a single-cell genomics database, we identified overexpressed biological process terms differentially expressed genes, such as “protein localization endosomes” “xenobiotic transport.” Overrepresented molecular function included “voltage-gated calcium channel activity,” “growth hormone-releasing hormone receptor “sialic acid transmembrane transporter activity.” Analysis revealed nine hub associated sperm: RPL34, CYB5B, GOL6A6, LSM1, ARL4A, DHX57, STARD9, HSP90B1 , VPS36 . Conclusions These their interacting proteins may role pathophysiology germ cell abnormalities

Язык: Английский

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Altered G-Protein Transduction Protein Gene Expression in the Testis of Infertile Patients with Nonobstructive Azoospermia

DNA and Cell Biology, Год журнала: 2023, Номер 42(10), С. 617 - 637

Опубликована: Авг. 23, 2023

Recent studies have shown that several members of the G-protein-coupled receptors (GPCR) superfamily play crucial roles in maintenance ion-water homeostasis sperm and Sertoli cells, development germ formation blood barrier, maturation sperm. The GPCR, guanyl-nucleotide exchange factor, membrane traffic protein, small GTPase genes were analyzed by microarray bioinformatics (3513 cell genes). In analyses three human cases with different nonobstructive azoospermia sperm, expression GOLGA8IP, OR2AT4, PHKA1, A2M, OR56A1, SEMA3G, LRRC17, APP, ARHGAP33, RABGEF1, NPY2R, GHRHR, LTB4R2, GRIK5, OR6K6, NAPG, OR6C65, VPS35, FPR3, ARL4A was upregulated, while MARS, SIRPG, OGFR, GPR150, LRRK1, NGEF downregulated. There an increase GBP3, TNF, TGFB3, CLTC cells NOA, whereas PAQR4, RRAGD, RAC2, SERPINB8, IRPB1, MRGPRF, RASA2, RGS2, RAP2A, RAB2B, ARL17, SERINC4, XIAP, DENND4C, ANKRA2, CSTA, STX18, SNAP23 A combined analysis Enrich Shiny Gene Ontology (GO), STRING, Cytoscape used to predict proteins' molecular interactions then recognize master pathways. Functional enrichment showed biological process (BP), regulation protein metabolic process, GTPase-mediated signal transduction significantly expressed up-/downregulated differentially (DEGs) function (MF) experiments DEGs up-/downregulated, it found GPCR activity, guanyl ribonucleotide binding, activity nucleoside-triphosphatase overexpressed. An GO findings BP MF be common DEGs. When these gene mutations been validated, they can create new antagonists or agonists are receptor-selective.

Язык: Английский

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Differential expression of ion channel coding genes in the endometrium of women experiencing recurrent implantation failures

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Авг. 27, 2024

Our study probed the differences in ion channel gene expression endometrium of women with Recurrent Implantation Failure (RIF) compared to fertile women. We analyzed relative genes coding for T-type Ca2+, ENaC, CFTR, and KCNQ1 channels endometrial samples from 20 RIF-affected 10 control women, aged 22-35, via microarray analysis quantitative real-time PCR. Additionally, we examined DNA methylation regulatory region using ChIP The bioinformatics component our research included Gene Ontology analysis, protein-protein interaction networks, signaling pathway mapping identify key biological processes pathways implicated RIF. This led discovery significant alterations RIF women's endometrium, most notably an overexpression CFTR reduced SCNN1A, SCNN1B, SCNN1G, CACNA1H, KCNQ1. A higher level KCNQ1's was also observed patients. Gene-set enrichment highlighted a presence involved transport membrane potential regulation, particularly sodium calcium complexes, which are vital cation movement across cell membranes. Genes were enriched broader transmembrane transporter underscoring their extensive role cellular homeostasis signaling. These findings suggest involvement pathology implantation failure, offering new insights into mechanisms behind possible therapeutic targets.

Язык: Английский

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Exploring the interaction between immune cells in the prostate cancer microenvironment combining weighted correlation gene network analysis and single-cell sequencing: An integrated bioinformatics analysis

Discover Oncology, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 30, 2024

Язык: Английский

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Identification of novel cytoskeleton protein involved in spermatogenic cells and sertoli cells of non-obstructive azoospermia based on microarray and bioinformatics analysis

BMC Medical Genomics, Год журнала: 2025, Номер 18(1)

Опубликована: Янв. 25, 2025

Язык: Английский

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Investigating the Expression and Role of N-Myc in Spermatogonial Stem Cells and Male Infertility

Annals of Anatomy - Anatomischer Anzeiger, Год журнала: 2025, Номер 259, С. 152391 - 152391

Опубликована: Фев. 11, 2025

Язык: Английский

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Analysis of microarray and single-cell RNA-seq identifies gene co-expression, cell–cell communication, and tumor environment associated with metabolite interconversion enzyme in prostate cancer

Discover Oncology, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 13, 2025

Prostate cancer (PCa) is the second most common malignant neoplasm in males and fifth leading cause of cancer-related mortality. Due to use prostate-specific antigen (PSA) screening improved biopsy techniques, persons identified with early-stage prostate often have a positive prognosis after comprehensive treatment. Nonetheless, latent illness that may present as an asymptomatic tumor individuals aged 20-30. The overall survival (OS) men advanced PCa significantly diminished. Consequently, there immediate want for innovative, accurate biomarkers detect early cancer. This research analyzed interaction network differentially expressed genes (DEGs) related metabolite interconversion enzymes by gene expression microarray data, single-cell RNA sequencing, oncogenes, suppressor (TSGs) utilizing bioinformatics techniques. kind analysis has not been documented prior studies. We then used dataset acquired Cancer Genome Atlas (TCGA) confirm our findings. Genes including CYP3A5, PDE8B, AOX1, BNIPL, FADS2, RRM2, ALDH3B2, GSTM2 be significant diagnosis treatment PCa. Our objective was provide new perspectives on molecular properties pathways DEGs uncover potential play crucial role genesis progression

Язык: Английский

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Integration of Microarray and Single-Cell RNA-Seq Data and Machine Learning Allows the Identification of Key Histone Modification Gene Changes in Spermatogonial Stem Cells

Biology, Год журнала: 2025, Номер 14(4), С. 387 - 387

Опубликована: Апрель 8, 2025

Histone modifications play a critical role in regulating gene expression and maintaining the functionality of spermatogonial stem cells (SSCs), which are essential for male fertility spermatogenesis. In this study, we integrated microarray single-cell RNA-sequencing (scRNA-seq) data to identify key histone modification changes associated with SSC function aging. Through differential analysis, identified 2509 differentially expressed genes (DEGs) SSCs compared fibroblasts. Among these, involved modification, such as KDM5B, SCML2, SIN3A, ASXL3, were highlighted their significant roles chromatin remodeling regulation. Protein–protein interaction (PPI) networks ontology (GO) enrichment analysis revealed biological processes organization, demethylation, chromosome structure maintenance. Weighted co-expression network (WGCNA) further three modules co-expressed related Additionally, ligand–receptor scoring based on tumor microenvironment suggested potential signaling pathways that could influence stemness differentiation SSCs. Our findings provide new insights into molecular mechanisms underlying aging, highlighting therapeutic targets preserving improving SSC-culturing techniques. This study advances our understanding biology will serve valuable resource future investigations preservation.

Язык: Английский

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Identification of novel long non-coding RNA involved in Sertoli cell of non-obstructive azoospermia based on microarray and bioinformatics analysis

Genomics, Год журнала: 2025, Номер 117(3), С. 111046 - 111046

Опубликована: Апрель 23, 2025

Non-obstructive azoospermia (NOA) is a severe form of male infertility, yet its underlying molecular mechanisms remain poorly understood. This study aimed to identify key regulatory non-coding RNAs (ncRNAs) and hub genes associated with NOA through an integrative bioinformatics approach. Using microarray analysis, we examined 4956 ncRNAs identified 29 differentially expressed (14 upregulated, 15 downregulated) in compared healthy individuals. Co-expression analysis revealed significant interactions between lncRNAs, miRNAs, mRNAs, predicting 31 target mRNAs within the network. Further, single-cell transcriptomic four pivotal Sertoli cells: CLTC, XIAP, DHFR (upregulated) STMN1 (downregulated). Functional enrichment highlighted critical pathways, including mitotic spindle organization phosphatase activity, suggesting their involvement pathophysiology. Our findings provide novel insights into propose potential biomarkers for improved diagnosis therapeutic strategies.

Язык: Английский

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