Exploring the Potential Role and Mechanism of Quercetin in the Treatment of Primary Sjögren's Syndrome Based on Network Pharmacology, Molecular Docking, and Experimental Validation
Abstract
Objective
This
study
investigates
Quercetin’s
effects
on
lymphocyte
infiltration
and
salivary
function
in
primary
Sjögren’s
Syndrome
(pSS)
mice
explores
its
mechanisms
via
network
pharmacology,
molecular
docking,
experimental
validation.
Methods
pSS
model
controls
were
divided
into
four
groups
(control,
model,
Quercetin,
hydroxychloroquine).
After
12
weeks,
saliva
flow
submandibular
gland
histopathology
assessed.
Network
pharmacology
identified
targets,
followed
by
GO/KEGG
analyses.
Molecular
docking
qPCR
validated
core
targets.
Results
Quercetin
reduced
infiltration,
improved
flow,
targeted
138
genes
(TP53,
TNF,
JUN,
AKT1,
IL6
as
core).
linked
targets
to
lipopolysaccharide
response,
oxidative
stress,
atherosclerosis
pathways.
showed
strong
binding
downregulated
Tp53/Jun
mRNA.
Conclusion
alleviates
damage
modulating
inflammation/immunity
pathways,
highlighting
therapeutic
potential.
supports
further
exploration
of
for
treatment.
Research Square (Research Square), Год журнала: 2025, Номер unknown
Опубликована: Март 13, 2025
Язык: Английский