Exploring the Potential Role and Mechanism of Quercetin in the Treatment of Primary Sjögren's Syndrome Based on Network Pharmacology, Molecular Docking, and Experimental Validation DOI Creative Commons
Jia Yin,

Hannah F. Hu,

Yinyue Xu

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract Objective This study investigates Quercetin’s effects on lymphocyte infiltration and salivary function in primary Sjögren’s Syndrome (pSS) mice explores its mechanisms via network pharmacology, molecular docking, experimental validation. Methods pSS model controls were divided into four groups (control, model, Quercetin, hydroxychloroquine). After 12 weeks, saliva flow submandibular gland histopathology assessed. Network pharmacology identified targets, followed by GO/KEGG analyses. Molecular docking qPCR validated core targets. Results Quercetin reduced infiltration, improved flow, targeted 138 genes (TP53, TNF, JUN, AKT1, IL6 as core). linked targets to lipopolysaccharide response, oxidative stress, atherosclerosis pathways. showed strong binding downregulated Tp53/Jun mRNA. Conclusion alleviates damage modulating inflammation/immunity pathways, highlighting therapeutic potential. supports further exploration of for treatment.

Language: Английский

Exploring the Potential Role and Mechanism of Quercetin in the Treatment of Primary Sjögren's Syndrome Based on Network Pharmacology, Molecular Docking, and Experimental Validation DOI Creative Commons
Jia Yin,

Hannah F. Hu,

Yinyue Xu

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract Objective This study investigates Quercetin’s effects on lymphocyte infiltration and salivary function in primary Sjögren’s Syndrome (pSS) mice explores its mechanisms via network pharmacology, molecular docking, experimental validation. Methods pSS model controls were divided into four groups (control, model, Quercetin, hydroxychloroquine). After 12 weeks, saliva flow submandibular gland histopathology assessed. Network pharmacology identified targets, followed by GO/KEGG analyses. Molecular docking qPCR validated core targets. Results Quercetin reduced infiltration, improved flow, targeted 138 genes (TP53, TNF, JUN, AKT1, IL6 as core). linked targets to lipopolysaccharide response, oxidative stress, atherosclerosis pathways. showed strong binding downregulated Tp53/Jun mRNA. Conclusion alleviates damage modulating inflammation/immunity pathways, highlighting therapeutic potential. supports further exploration of for treatment.

Language: Английский

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