Metabolic Brain Disease, Год журнала: 2024, Номер 40(1)
Опубликована: Ноя. 26, 2024
Язык: Английский
Functional & Integrative Genomics, Год журнала: 2024, Номер 24(5)
Опубликована: Сен. 26, 2024
Язык: Английский
Процитировано
12
Functional & Integrative Genomics, Год журнала: 2025, Номер 25(1)
Опубликована: Янв. 17, 2025
Язык: Английский
Процитировано
1
Frontiers in Immunology, Год журнала: 2025, Номер 16
Опубликована: Янв. 24, 2025
Background Long non-coding RNAs (lncRNAs) in extracellular vesicles (EVs) have been confirmed as effective non-invasive biomarkers for multiple diseases. However, their expression and clinical value gastric cancer (GC) remain poorly understood. Materials methods Serum EV RNA was extracted from four patients with GC healthy controls, followed by high-throughput sequencing. LncRNAs were further validated training validation sets using quantitative real-time reverse transcription polymerase chain reaction. Results A total of 37,684 lncRNAs obtained, 10 selected based on the criteria ( P < 0.05 |log 2 FoldChange| ≥1). lncRNA RMRP, RPPH1, linc-ROR significantly higher than those chronic gastritis, atypical hyperplasia, or control (all 0.05). Three also correlated tumor diameter, lymphatic metastasis, distal TNM stage The area under curve (AUC) values 0.727, 0.774, 0.811, respectively. Corresponding sensitivity specificity 63.4% 85.4%, 50.7% 89.6%, 78.5% 66.7%. combination these three carcinoembryonic antigen (CEA) yielded an AUC 0.909, a 83.3% each. Furthermore, high RMRP levels associated worse disease-free survival overall (OS). Univariate multivariate Cox regression analyses that only independent prognostic factor GC. Finally, lncRNA-miRNA-mRNA network showed predicted to interact 15 miRNAs 69 mRNAs. In addition, immune cell infiltration, including neutrophils, central memory CD4 T cells, macrophage, natural kill cells. Conclusion are prospective biomarker infiltration It provides foundation development serum EV-targeted novel immunotherapy targets
Язык: Английский
Процитировано
1
Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2024, Номер unknown
Опубликована: Сен. 17, 2024
Язык: Английский
Процитировано
5
Journal of Biochemical and Molecular Toxicology, Год журнала: 2025, Номер 39(3)
Опубликована: Фев. 23, 2025
ABSTRACT Gastric cancer (GC) represents a major global health concern, with over 1 million new cases diagnosed annually worldwide. Emerging studies have highlighted the significant correlation between long noncoding RNAs (lncRNAs) and progression of GC. The objective current study is to investigate roles mechanism lncRNA homeobox A10 antisense RNA (HOXA10‐AS) in modulating malignant properties GC cells. RT‐qPCR was employed detect HOXA10‐AS expression cells or human normal gastric epithelium cellular localization mRNA HOXA10 were detected using fractionation assays. Colony forming assays Transwell performed assess proliferative, invasive, migratory capabilities Western blot analysis used determine protein levels epithelial mesenchymal transition (EMT) markers immunoprecipitation, pulldown luciferase conducted explore gene interaction. As shown by experimental results, showed high silencing led weakened abilities cells, as well inhibition EMT process. Moreover, positively regulated interacting miR‐29a/b/c‐3p. Additionally, overexpression counteracted repressive impacts on process caused knockdown HOXA10‐AS. Furthermore, activated p38 MAPK/STAT3 signaling pathway via upregulation HOXA10. In conclusion, upregulates through interaction resultant increase activates signaling, thereby promoting cell growth, migration, invasion,
Язык: Английский
Процитировано
0
Advances in Clinical Medicine, Год журнала: 2025, Номер 15(03), С. 2240 - 2245
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Clinical Rheumatology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 25, 2025
Язык: Английский
Процитировано
0
Scientific Reports, Год журнала: 2025, Номер 15(1)
Опубликована: Май 18, 2025
Colorectal cancer (CRC) progression involves complex molecular alterations, including the dysregulation of long non-coding RNAs (lncRNAs). In this study, we identified key progression-related lncRNAs in CRC by integrating transcriptomic data from TCGA and single-cell RNA sequencing (scRNA-seq). Differential expression analysis revealed numerous associated with progression. To systematically prioritize these lncRNAs, developed a scoring system incorporating multiple signatures, differential expression, survival analysis. This approach 198 both known (e.g., LINC01615) novel candidates AC007998.3). Experimental validation confirmed that LINC01615 was significantly upregulated tissues, whereas AC007998.3 downregulated. Further analyses indicated influence through cis-, trans-, post-transcriptional regulation. Patients were classified into distinct subgroups based on lncRNA exhibiting significant differences prognosis immune microenvironment composition. The enrichment among differentially expressed statistically significant, reinforcing their functional relevance. Validation across independent datasets demonstrated robustness our findings. Our research provides insights mechanisms underlying highlights potential as prognostic biomarkers therapeutic targets.
Язык: Английский
Процитировано
0
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4842 - 4842
Опубликована: Май 19, 2025
Circular RNAs (circRNAs) are increasingly recognized as significant regulators in multiple cancers, such hepatocellular carcinoma (HCC), frequently affecting microRNA (miRNA) expression. The diagnostic and prognostic roles of circRNAs, specifically circ_0009910 circ_0027478, conjunction with miR-1236-3p, HCC, have not yet been fully investigated. In this pilot study, we assessed the expression levels circ_0009910, miR-1236-3p plasma samples from 100 patients diagnosed HCC 50 healthy controls through reverse transcriptase–quantitative polymerase chain reaction (RT-qPCR). performance was evaluated using receiver operating characteristic (ROC) curve analysis, correlations clinicopathological features were examined. Circ_0009910 circ_0027478 exhibited upregulation (p < 0.05), whereas demonstrated downregulation 0.05). accuracy (area under [AUC] = 0.90), effectively differentiating showing a correlation tumor size, metastasis, alpha-fetoprotein (AFP) Both positive features, an inverse correlation. Logistic regression validated capabilities these biomarkers. results indicate that AFP three, present promising profile for HCC. Additional validation larger cohorts is required to establish their clinical utility.
Язык: Английский
Процитировано
0
Metabolic Brain Disease, Год журнала: 2024, Номер 40(1)
Опубликована: Ноя. 26, 2024
Язык: Английский
Процитировано
2