Metabolic Brain Disease, Journal Year: 2024, Volume and Issue: 40(1)
Published: Nov. 26, 2024
Language: Английский
Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(5)
Published: Sept. 26, 2024
Language: Английский
Citations
9
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: Jan. 24, 2025
Background Long non-coding RNAs (lncRNAs) in extracellular vesicles (EVs) have been confirmed as effective non-invasive biomarkers for multiple diseases. However, their expression and clinical value gastric cancer (GC) remain poorly understood. Materials methods Serum EV RNA was extracted from four patients with GC healthy controls, followed by high-throughput sequencing. LncRNAs were further validated training validation sets using quantitative real-time reverse transcription polymerase chain reaction. Results A total of 37,684 lncRNAs obtained, 10 selected based on the criteria ( P < 0.05 |log 2 FoldChange| ≥1). lncRNA RMRP, RPPH1, linc-ROR significantly higher than those chronic gastritis, atypical hyperplasia, or control (all 0.05). Three also correlated tumor diameter, lymphatic metastasis, distal TNM stage The area under curve (AUC) values 0.727, 0.774, 0.811, respectively. Corresponding sensitivity specificity 63.4% 85.4%, 50.7% 89.6%, 78.5% 66.7%. combination these three carcinoembryonic antigen (CEA) yielded an AUC 0.909, a 83.3% each. Furthermore, high RMRP levels associated worse disease-free survival overall (OS). Univariate multivariate Cox regression analyses that only independent prognostic factor GC. Finally, lncRNA-miRNA-mRNA network showed predicted to interact 15 miRNAs 69 mRNAs. In addition, immune cell infiltration, including neutrophils, central memory CD4 T cells, macrophage, natural kill cells. Conclusion are prospective biomarker infiltration It provides foundation development serum EV-targeted novel immunotherapy targets
Language: Английский
Citations
1
Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 17, 2024
Language: Английский
Citations
4
Functional & Integrative Genomics, Journal Year: 2025, Volume and Issue: 25(1)
Published: Jan. 17, 2025
Language: Английский
Citations
0
Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(3)
Published: Feb. 23, 2025
ABSTRACT Gastric cancer (GC) represents a major global health concern, with over 1 million new cases diagnosed annually worldwide. Emerging studies have highlighted the significant correlation between long noncoding RNAs (lncRNAs) and progression of GC. The objective current study is to investigate roles mechanism lncRNA homeobox A10 antisense RNA (HOXA10‐AS) in modulating malignant properties GC cells. RT‐qPCR was employed detect HOXA10‐AS expression cells or human normal gastric epithelium cellular localization mRNA HOXA10 were detected using fractionation assays. Colony forming assays Transwell performed assess proliferative, invasive, migratory capabilities Western blot analysis used determine protein levels epithelial mesenchymal transition (EMT) markers immunoprecipitation, pulldown luciferase conducted explore gene interaction. As shown by experimental results, showed high silencing led weakened abilities cells, as well inhibition EMT process. Moreover, positively regulated interacting miR‐29a/b/c‐3p. Additionally, overexpression counteracted repressive impacts on process caused knockdown HOXA10‐AS. Furthermore, activated p38 MAPK/STAT3 signaling pathway via upregulation HOXA10. In conclusion, upregulates through interaction resultant increase activates signaling, thereby promoting cell growth, migration, invasion,
Language: Английский
Citations
0
Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(03), P. 2240 - 2245
Published: Jan. 1, 2025
Language: Английский
Citations
0
Metabolic Brain Disease, Journal Year: 2024, Volume and Issue: 40(1)
Published: Nov. 26, 2024
Language: Английский
Citations
1