Journal of Biomedical Science,
Год журнала:
2024,
Номер
31(1)
Опубликована: Авг. 13, 2024
Abstract
Gene
therapy
has
made
considerable
strides
in
recent
years.
More
than
4000
protein-coding
genes
have
been
implicated
more
6000
genetic
diseases;
next-generation
sequencing
dramatically
revolutionized
the
diagnosis
of
diseases.
Most
diseases
are
considered
very
rare
or
ultrarare,
defined
here
as
having
fewer
1:100,000
cases,
but
only
one
12
approved
gene
therapies
(excluding
RNA
therapies)
targets
an
ultrarare
disease.
This
article
explores
three
supplementation
approaches
suitable
for
various
diseases:
lentiviral
vector-modified
autologous
CD34
+
hematopoietic
stem
cell
transplantation,
systemic
delivery
adeno-associated
virus
(AAV)
vectors
to
liver,
and
local
AAV
cerebrospinal
fluid
brain.
Together
with
therapies,
we
propose
a
potential
business
model
these
therapies.
BMC Infectious Diseases,
Год журнала:
2024,
Номер
24(1)
Опубликована: Июнь 3, 2024
Abstract
Background
and
objective(s)
CRISPR-Cas
is
a
prokaryotic
adaptive
immune
system
that
protects
bacteria
archaea
against
mobile
genetic
elements
(MGEs)
such
as
bacteriophages
plasmids,
transposons.
In
this
study,
we
aimed
to
assess
the
prevalence
of
systems
their
association
with
antibiotic
resistance
in
one
most
challenging
bacterial
pathogens,
Klebsiella
pneumoniae
.
Materials
methods
A
total
105
K.
isolates
were
collected
from
various
clinical
infections.
Extended-spectrum
β-lactamases
(ESBLs)
phenotypically
detected
presence
ESBL,
aminoglycoside-modifying
enzymes
(AME),
subtype
genes
identified
using
PCR.
Moreover,
diversity
was
determined
by
enterobacterial
repetitive
intergenic
consensus
(ERIC)-PCR.
Results
Phenotypically,
41.9%
(44/105)
found
be
ESBL
producers.
significant
inverse
correlation
existed
between
I-E
system’s
production
isolates.
Additionally,
frequency
bla
CTX−M1
(3%),
CTX−M9
(12.1%),
SHV
(51.5%),
TEM
(33.3%),
well
some
AME
aac(3)-Iva
(21.2%)
ant(2’’)-Ia
(3%)
significantly
lower
comparison
CRISPR-negative
There
system.
Conclusion
The
correlated
antibiotic-resistant
gene
(ARGs).
had
than
Aquaculture and Fisheries,
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 1, 2024
Aquaculture,
a
critical
sector
for
global
food
security,
faces
the
challenge
of
meeting
growing
demand
while
protecting
wild
fish
populations.
Gene
editing,
powerful
genetic
tool,
emerges
as
potential
solution.
By
modifying
key
genes
in
fish,
it
is
expected
to
rapidly
enhance
growth
rate,
disease
resistance,
and
other
economically
important
traits,
leading
increased
profitability,
sustainability,
competitiveness
industry.
This
review
navigates
complex
landscape
gene
editing
aquaculture,
exploring
successes,
challenges,
prospects
improved
productivity,
resilience.
We
provide
guidance
researchers
stakeholders
identifying
responsible
addressing
economic,
environmental,
regulatory,
ethical
considerations.
roadmap
paves
way
future
where
empowers
aquaculture
meet
needs
upholding
environmental
responsibility.
Journal of Biomedical Science,
Год журнала:
2024,
Номер
31(1)
Опубликована: Авг. 13, 2024
Abstract
Gene
therapy
has
made
considerable
strides
in
recent
years.
More
than
4000
protein-coding
genes
have
been
implicated
more
6000
genetic
diseases;
next-generation
sequencing
dramatically
revolutionized
the
diagnosis
of
diseases.
Most
diseases
are
considered
very
rare
or
ultrarare,
defined
here
as
having
fewer
1:100,000
cases,
but
only
one
12
approved
gene
therapies
(excluding
RNA
therapies)
targets
an
ultrarare
disease.
This
article
explores
three
supplementation
approaches
suitable
for
various
diseases:
lentiviral
vector-modified
autologous
CD34
+
hematopoietic
stem
cell
transplantation,
systemic
delivery
adeno-associated
virus
(AAV)
vectors
to
liver,
and
local
AAV
cerebrospinal
fluid
brain.
Together
with
therapies,
we
propose
a
potential
business
model
these
therapies.
