BMC Infectious Diseases,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: June 3, 2024
Abstract
Background
and
objective(s)
CRISPR-Cas
is
a
prokaryotic
adaptive
immune
system
that
protects
bacteria
archaea
against
mobile
genetic
elements
(MGEs)
such
as
bacteriophages
plasmids,
transposons.
In
this
study,
we
aimed
to
assess
the
prevalence
of
systems
their
association
with
antibiotic
resistance
in
one
most
challenging
bacterial
pathogens,
Klebsiella
pneumoniae
.
Materials
methods
A
total
105
K.
isolates
were
collected
from
various
clinical
infections.
Extended-spectrum
β-lactamases
(ESBLs)
phenotypically
detected
presence
ESBL,
aminoglycoside-modifying
enzymes
(AME),
subtype
genes
identified
using
PCR.
Moreover,
diversity
was
determined
by
enterobacterial
repetitive
intergenic
consensus
(ERIC)-PCR.
Results
Phenotypically,
41.9%
(44/105)
found
be
ESBL
producers.
significant
inverse
correlation
existed
between
I-E
system’s
production
isolates.
Additionally,
frequency
bla
CTX−M1
(3%),
CTX−M9
(12.1%),
SHV
(51.5%),
TEM
(33.3%),
well
some
AME
aac(3)-Iva
(21.2%)
ant(2’’)-Ia
(3%)
significantly
lower
comparison
CRISPR-negative
There
system.
Conclusion
The
correlated
antibiotic-resistant
gene
(ARGs).
had
than
Journal of Experimental Botany,
Journal Year:
2023,
Volume and Issue:
74(15), P. 4349 - 4366
Published: May 19, 2023
Abstract
Gene
expression
plays
a
fundamental
role
in
the
regulation
of
agronomically
important
traits
crop
plants.
The
genetic
manipulation
plant
promoters
through
genome
editing
has
emerged
as
an
effective
strategy
to
create
favorable
crops
by
altering
pattern
pertinent
genes.
Promoter
can
be
applied
directed
manner,
where
nucleotide
sequences
associated
with
are
precisely
generated.
Alternatively,
promoter
also
exploited
random
mutagenic
approach
generate
novel
variations
within
designated
promoter,
from
which
elite
alleles
selected
based
on
their
phenotypic
effects.
Pioneering
studies
have
demonstrated
potential
engineering
well
mining
valuable
for
breeding.
In
this
review,
we
provide
update
application
increased
yield,
enhanced
tolerance
biotic
and
abiotic
stresses,
improved
quality.
We
discuss
several
remaining
technical
bottlenecks
how
may
better
employed
improvement
future.
Aquaculture and Fisheries,
Journal Year:
2024,
Volume and Issue:
unknown
Published: July 1, 2024
Aquaculture,
a
critical
sector
for
global
food
security,
faces
the
challenge
of
meeting
growing
demand
while
protecting
wild
fish
populations.
Gene
editing,
powerful
genetic
tool,
emerges
as
potential
solution.
By
modifying
key
genes
in
fish,
it
is
expected
to
rapidly
enhance
growth
rate,
disease
resistance,
and
other
economically
important
traits,
leading
increased
profitability,
sustainability,
competitiveness
industry.
This
review
navigates
complex
landscape
gene
editing
aquaculture,
exploring
successes,
challenges,
prospects
improved
productivity,
resilience.
We
provide
guidance
researchers
stakeholders
identifying
responsible
addressing
economic,
environmental,
regulatory,
ethical
considerations.
roadmap
paves
way
future
where
empowers
aquaculture
meet
needs
upholding
environmental
responsibility.
BMC Infectious Diseases,
Journal Year:
2024,
Volume and Issue:
24(1)
Published: June 3, 2024
Abstract
Background
and
objective(s)
CRISPR-Cas
is
a
prokaryotic
adaptive
immune
system
that
protects
bacteria
archaea
against
mobile
genetic
elements
(MGEs)
such
as
bacteriophages
plasmids,
transposons.
In
this
study,
we
aimed
to
assess
the
prevalence
of
systems
their
association
with
antibiotic
resistance
in
one
most
challenging
bacterial
pathogens,
Klebsiella
pneumoniae
.
Materials
methods
A
total
105
K.
isolates
were
collected
from
various
clinical
infections.
Extended-spectrum
β-lactamases
(ESBLs)
phenotypically
detected
presence
ESBL,
aminoglycoside-modifying
enzymes
(AME),
subtype
genes
identified
using
PCR.
Moreover,
diversity
was
determined
by
enterobacterial
repetitive
intergenic
consensus
(ERIC)-PCR.
Results
Phenotypically,
41.9%
(44/105)
found
be
ESBL
producers.
significant
inverse
correlation
existed
between
I-E
system’s
production
isolates.
Additionally,
frequency
bla
CTX−M1
(3%),
CTX−M9
(12.1%),
SHV
(51.5%),
TEM
(33.3%),
well
some
AME
aac(3)-Iva
(21.2%)
ant(2’’)-Ia
(3%)
significantly
lower
comparison
CRISPR-negative
There
system.
Conclusion
The
correlated
antibiotic-resistant
gene
(ARGs).
had
than
