Brain and Behavior,
Год журнала:
2025,
Номер
15(5)
Опубликована: Май 1, 2025
ABSTRACT
Purpose
After
traumatic
brain
injury
(TBI),
ischemia
and
hypoxia
of
tissue,
glucose
undergoes
anaerobic
fermentation,
leading
to
a
large
accumulation
lactic
acid.
Our
aim
was
explore
the
role
lactate
metabolism
in
cells
after
TBI.
Method
In
scRNA‐seq
dataset,
10‐week‐old
male
C57BL/6
J
mice
were
randomized
undergo
mild
fluid
percussion
or
sham
surgery,
we
analyzed
frontal
cortex
tissue
during
acute
(24
h)
subacute
(7
days)
phases
TBI
at
single‐cell
resolution.
Cell
cycle
evaluated,
principal
component
analysis
performed.
populations
identified
visualized
using
UMAP
downscaling
technique.
Differentially
expressed
genes
(DEGs)
“FindAllMarkers”
algorithm.
addition,
set
related
evaluated
AUCell
score.
GO
KEGG
enrichment
analyses
performed
investigate
functional
pathways
DEGs
astrocytes
Results
A
total
13
cell
distinguished,
including
neurons,
astrocytes,
oligodendrocyte
progenitors.
The
number
endothelial
reduced
group
compared
with
group.
During
phase
TBI,
enhanced
interactions
between
brain‐associated
cells,
especially
precursor
observed.
Several
signaling
pathways,
EGF,
CSF,
MIF
inflammatory
factors
as
well
PSAP
PTN
neurotrophic
factor
significantly
Lactate
scores
elevated
group,
astrocytes.
phase,
frequency
intercellular
communication
increased
but
its
intensity
decreased.
Astrocytes
remained
high
levels
both
phases.
closely
associated
Subsequently,
NADH:ubiquinone
oxidoreductase
subunit
B9
(
Ndufb9
)
cytochrome
c
oxidase
8A
Cox8a
key
players
showed
consistent
upward
trend
following
transcriptomic
data.
Conclusion
play
an
important
These
findings
provide
new
insights
into
cellular
molecular
mechanisms
Molecular Neurobiology,
Год журнала:
2024,
Номер
61(10), С. 1 - 13
Опубликована: Март 20, 2024
Neuroblasts
were
first
derived
from
the
adult
mammalian
brains
in
1990s
by
Reynolds
et
al.
Since
then,
persistent
neurogenesis
subgranular
zone
(SGZ)
of
hippocampus
and
subventricular
(SVZ)
has
gradually
been
recognized.
To
date,
reviews
on
neuroblast
migration
have
largely
investigated
glial
cells
molecular
signaling
mechanisms,
while
relationship
between
vasculature
cell
remains
a
mystery.
Thus,
this
paper
underlines
partial
biological
features
unravels
significance
mechanisms
process
to
further
clarify
theoretically
neural
repair
mechanism
after
brain
injury.
Neuroblast
presents
three
modes
according
characteristics
that
act
as
scaffolds
during
process:
gliophilic
migration,
neurophilic
vasophilic
migration.
Many
molecules,
including
brain-derived
neurotrophic
factor
(BDNF),
stromal
cell-derived
1
(SDF-1),
vascular
endothelial
growth
(VEGF),
angiopoietin-1
(Ang-1),
affect
synergistically
regulating
neuroblasts
target
areas
along
blood
vessels.
However,
precise
role
vessels
needs
be
explored.
The
in-depth
study
will
most
probably
provide
theoretical
basis
breakthrough
for
clinical
treatment
injury
diseases.
Lipids in Health and Disease,
Год журнала:
2024,
Номер
23(1)
Опубликована: Июнь 27, 2024
Traumatic
brain
injury
(TBI)
causes
neuroinflammation
and
can
lead
to
long-term
neurological
dysfunction,
even
in
cases
of
mild
TBI
(mTBI).
Despite
the
substantial
burden
this
disease,
management
is
precluded
by
an
incomplete
understanding
its
cellular
mechanisms.
Sphingolipids
(SPL)
their
metabolites
have
emerged
as
key
orchestrators
biological
processes
related
tissue
injury,
neuroinflammation,
inflammation
resolution.
No
study
so
far
has
investigated
comprehensive
sphingolipid
profile
changes
immediately
following
animal
models
or
human
cases.
In
study,
metabolite
composition
was
examined
during
acute
phases
plasma
mice
mTBI.
Brain and Behavior,
Год журнала:
2025,
Номер
15(5)
Опубликована: Май 1, 2025
ABSTRACT
Purpose
After
traumatic
brain
injury
(TBI),
ischemia
and
hypoxia
of
tissue,
glucose
undergoes
anaerobic
fermentation,
leading
to
a
large
accumulation
lactic
acid.
Our
aim
was
explore
the
role
lactate
metabolism
in
cells
after
TBI.
Method
In
scRNA‐seq
dataset,
10‐week‐old
male
C57BL/6
J
mice
were
randomized
undergo
mild
fluid
percussion
or
sham
surgery,
we
analyzed
frontal
cortex
tissue
during
acute
(24
h)
subacute
(7
days)
phases
TBI
at
single‐cell
resolution.
Cell
cycle
evaluated,
principal
component
analysis
performed.
populations
identified
visualized
using
UMAP
downscaling
technique.
Differentially
expressed
genes
(DEGs)
“FindAllMarkers”
algorithm.
addition,
set
related
evaluated
AUCell
score.
GO
KEGG
enrichment
analyses
performed
investigate
functional
pathways
DEGs
astrocytes
Results
A
total
13
cell
distinguished,
including
neurons,
astrocytes,
oligodendrocyte
progenitors.
The
number
endothelial
reduced
group
compared
with
group.
During
phase
TBI,
enhanced
interactions
between
brain‐associated
cells,
especially
precursor
observed.
Several
signaling
pathways,
EGF,
CSF,
MIF
inflammatory
factors
as
well
PSAP
PTN
neurotrophic
factor
significantly
Lactate
scores
elevated
group,
astrocytes.
phase,
frequency
intercellular
communication
increased
but
its
intensity
decreased.
Astrocytes
remained
high
levels
both
phases.
closely
associated
Subsequently,
NADH:ubiquinone
oxidoreductase
subunit
B9
(
Ndufb9
)
cytochrome
c
oxidase
8A
Cox8a
key
players
showed
consistent
upward
trend
following
transcriptomic
data.
Conclusion
play
an
important
These
findings
provide
new
insights
into
cellular
molecular
mechanisms
