Stem cell-derived extracellular vesicles in the therapeutic intervention of Alzheimer's Disease, Parkinson's Disease, and stroke

Theranostics, Год журнала: 2024, Номер 14(8), С. 3358 - 3384

Опубликована: Янв. 1, 2024

With the increase in aging population, occurrence of neurological disorders is rising.Recently, stem cell therapy has garnered attention due to its convenient sourcing, minimal invasiveness, and capacity for directed differentiation.However, there are some disadvantages, such as poor quality control, safety assessments, ethical logistical issues.Consequently, scientists have started shift their from cells extracellular vesicles similar structures properties.Beyond these parallels, can enhance biocompatibility, facilitate easy traversal barriers, minimize side effects.Furthermore, cell-derived be engineered load drugs modify surfaces treatment outcomes.In this review, we summarize functions native vesicles, subsequently review strategies engineering applications Alzheimer's disease, Parkinson's stroke, discuss challenges solutions associated with clinical translation vesicles.

Язык: Английский

---

Identification of key genes associated with oxidative stress in ischemic stroke via bioinformatics integrated analysis

BMC Neuroscience, Год журнала: 2025, Номер 26(1)

Опубликована: Янв. 13, 2025

Ischemic stroke (IS) is a common cerebrovascular disease. Although the formation of atherosclerosis, which closely related to oxidative stress (OS), associated with stroke-related deaths. However, role OS in IS unknown. OS-related key genes were obtianed by overlapping differentially expressed (DEGs) between and normal control (NC) specimens, IS-related genes, genes. Then, we investigated mechanism action Subsequently, protein–protein interaction (PPI) network machine learning algorithms utilized excavate feature In addition, microRNAs (miRNAs) was established investigate regulatory Finally, quantitative PCR (qPCR) validate expression blood specimens. A total 42 acquired. Enrichment analysis indicated that stress, reactive oxygen species, lipid cell migration-related pathways. 6 (HSPA8, NCF2, FOS, KLF4, THBS1, HSPA1A) identified for IS. Besides, 255 miRNAs establish genes-miRNA contained 261 nodes 277 edges. At last, qPCR results revealed there trend higher HSPA1A specimens than NC Additionally, HSPA8 significantly decreased consistent findings GEO database analysis. conclusion, mined bioinformatics analysis, might provide new insights into evaluation treatment Clinical trial number: Not applicable.

Язык: Английский

---

A Review of the Neuroprotective Properties of Exosomes Derived from Stem Cells and Exosome-Coated Nanoparticles for Treating Neurodegenerative Diseases and Stroke

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(8), С. 3915 - 3915

Опубликована: Апрель 21, 2025

Neurological diseases, including neurodegenerative disorders and stroke, represent significant medical challenges due to their complexity the limitations of current treatment approaches. This review explores potential stem cell (SC)-derived exosomes (Exos) as a transformative therapeutic strategy for these diseases. Exos, especially those derived from SCs, exhibit natural targeting ability, biocompatibility, capacity cross blood-brain barrier (BBB), making them ideal vehicles drug delivery. provides an in-depth discussion properties advantages SC-Exos. It highlights synergistic benefits in approaches treat neurological article discusses mechanisms action SC-Exos, highlighting ability target specific cells, modulate disease pathways, provide controlled release agents. Applications have been investigated, demonstrating improve outcomes conditions such Alzheimer's Disease (AD), Parkinson's (PD), stroke. Moreover, Exos-coated nanoparticles (NPs) combine Exos with multifunctionality NPs. integration takes advantage exosome membrane biocompatibility capabilities while preserving NPs' beneficial features, loading release. As result, NPs may enhance precision, efficacy, safety interventions. In conclusion, SC-Exos promising innovative approach treating

Язык: Английский

---

Epitranscriptomic modifications in mesenchymal stem cell differentiation: advances, mechanistic insights, and beyond

Cell Death and Differentiation, Год журнала: 2023, Номер 31(1), С. 9 - 27

Опубликована: Ноя. 20, 2023

Язык: Английский

---

METTL3-dependent N6-methyladenosine modification is involved in berberine-mediated neuroprotection in ischemic stroke by enhancing the stability of NEAT1 in astrocytes

Aging, Год журнала: 2024, Номер unknown

Опубликована: Янв. 4, 2024

Ischemic stroke (IS) is one of the principal causes disability and death worldwide. Berberine (BBR), derived from traditional Chinese herbal medicine Huang Lian, has been reported to inhibit progression stroke, but specific mechanism whereby BBR modulates ischemic remains unclear. N6-methyladenosine (m6A) modification most typical epigenetic mRNA post-transcriptional modifications, among which METTL3 common methylation transferase. During study, middle cerebral artery occlusion/reperfusion (MCAO/R) was established in mice, mice primary astrocytes neurons induced by oxygen-glucose deprivation/reoxygenation (OGD/R) simulated vitro. Level LncNEAT1, miR-377-3p detected via RT-qPCR. The levels Nampt were measured Western blot. CCK8 LDH assay performed detect cell viability. Here, we found that berberine alleviates MCAO/R-induced injury up-regulates expression astrocytes, inhibits after OGD/R, thus promoting neuronal injury. NEAT1 binds OGD/R plays a protective role as ceRNA. can enhance stability modulating m6A NEAT1. Taken together, our results demonstrate exerts neuroprotective effects methyltransferase METTL3, regulates NEAT1/miR-377-3p/Nampt axis mouse ameliorate ischemia/reperfusion (I/R)

Язык: Английский

---

Advancements in the treatment of cerebral ischemia-reperfusion injury: Acupuncture combined with mesenchymal stem cells transplantation

Medicine, Год журнала: 2025, Номер 104(2), С. e41075 - e41075

Опубликована: Янв. 10, 2025

Cerebral ischemia-reperfusion injury (CIRI) constitutes a significant etiology of exacerbated cerebral tissue damage subsequent to intravenous thrombolysis and endovascular mechanical thrombectomy in patients diagnosed with acute ischemic stroke. The treatment CIRI has been extensively investigated through multitude clinical studies. Acupuncture demonstrated be effective treating CIRI. Recent 5 years studies have identified potential mechanisms acupuncture, including regulation autophagy, promotion angiogenesis, inhibition inflammation apoptosis, modulation cell activation, neuroplasticity regulation, nerve regeneration. transplantation mesenchymal stem cells (MSCs) can effectively suppress modulate immune responses, enhance the proliferation migration endogenous neural (NSCs), thereby compensating for NSCs deficiency following ischemia/reperfusion injury. combination acupuncture MSCs demonstrates superiority over individual treatments, significantly enhancing survival rate MSCs. Moreover, it facilitates secretion various cytokines promote their homing differentiation into functional neurons, providing novel approach

Язык: Английский

---

Neuroprotective effects of exosomes derived from bone marrow mesenchymal stem cells treated by Musk Ketone on ischemic stroke

Journal of Stroke and Cerebrovascular Diseases, Год журнала: 2024, Номер 33(8), С. 107628 - 107628

Опубликована: Фев. 9, 2024

Язык: Английский

---

Effects of Bone Marrow Mesenchymal Stem Cell-Derived Exosomes in Central Nervous System Diseases

Molecular Neurobiology, Год журнала: 2024, Номер 61(10), С. 7481 - 7499

Опубликована: Фев. 23, 2024

Язык: Английский

---

Therapeutic gene delivery by mesenchymal stem cell for brain ischemia damage: Focus on molecular mechanisms in ischemic stroke

Cell Biochemistry and Function, Год журнала: 2024, Номер 42(2)

Опубликована: Март 1, 2024

Abstract Cerebral ischemic damage is prevalent and the second highest cause of death globally across patient populations; it as a substantial reason morbidity mortality. Mesenchymal stromal cells (MSCs) have garnered significant interest potential treatment for cerebral damage, shown in stroke, because their potent intrinsic features, which include self‐regeneration, immunomodulation, multi‐potency. Additionally, MSCs are easily obtained, isolated, cultured. Despite this, there number obstacles that hinder effectiveness MSC‐based treatment, such adverse microenvironmental conditions both vivo vitro. To overcome these obstacles, naïve MSC has undergone modification processes to enhance its innate therapeutic qualities. Genetic preconditioning (with medications, growth factors, other substances) two main categories into techniques can be separated. This field advanced significantly still attracting attention innovation. We examine cutting‐edge methods preserving even improving natural biological functions relation adhesion, migration, homing target site, survival, delayed premature senescence. address use genetically altered stroke‐induced damage. Future strategies result addressing difficulties associated with also discussed.

Язык: Английский

---

Exosomes: A Cellular Communication Medium That Has Multiple Effects On Brain Diseases

Molecular Neurobiology, Год журнала: 2024, Номер 61(9), С. 6864 - 6892

Опубликована: Фев. 14, 2024

Язык: Английский

---